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Fc blocked anti cd16 cd32

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The Fc-blocked (anti-CD16/CD32) lab equipment is a product designed to block Fc receptors, specifically CD16 and CD32, on cells. It serves as a tool for immunological studies and applications.

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Lab products found in correlation

Macsquant, by Miltenyi Biotec (2 mentions)

Spelling variants of this product

Anti-CD16/CD32 (62 citations) CD16/CD32 (23 citations) Fc-blocked (7 citations)

2 protocols using fc blocked anti cd16 cd32

1

Isolation and Characterization of Liver Immune Cells

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Following liver perfusion via the portal vein, tissue was dissociated in PBS with 10% fetal calf serum. Following a low speed spin to pellet hepatocytes, the supernatant was subject to a 40% Percoll gradient to isolate liver mononuclear cells (MNC). MNC were passed twice through 70 μm filters. Single-cell suspensions in PBS were incubated with viability dye, Fc-blocked (anti-CD16/CD32; eBioscience), and stained with mAbs to CD45, CD4, CD8α, TCRβ, CD11b, CD49b (clone DX5), GR-1, CD11c, and CD19 (Biolegend). iNKT cells were identified using PBS-57 tetramer, with empty CD1d tetramer as control (NIH tetramer facility). Cells were washed and fixed in 1% paraformaldehyde and acquired on a MACSQuant (Miltenyi). Data analysis used FlowJo.
Celaj S., Gleeson M.W., Deng J., O'Toole G.A., Hampton T.H., Toft M.F., Morrison H.G., Sogin M.L., Putra J., Suriawinata A.A, & Gorham J.D. (2014). The microbiota regulates susceptibility to Fas-mediated acute hepatic injury. Laboratory investigation; a journal of technical methods and pathology, 94(9), 938-949.
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2

Isolation and Characterization of Liver Immune Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols
Following liver perfusion via the portal vein, tissue was dissociated in PBS with 10% fetal calf serum. Following a low speed spin to pellet hepatocytes, the supernatant was subject to a 40% Percoll gradient to isolate liver mononuclear cells (MNC). MNC were passed twice through 70 μm filters. Single-cell suspensions in PBS were incubated with viability dye, Fc-blocked (anti-CD16/CD32; eBioscience), and stained with mAbs to CD45, CD4, CD8α, TCRβ, CD11b, CD49b (clone DX5), GR-1, CD11c, and CD19 (Biolegend). iNKT cells were identified using PBS-57 tetramer, with empty CD1d tetramer as control (NIH tetramer facility). Cells were washed and fixed in 1% paraformaldehyde and acquired on a MACSQuant (Miltenyi). Data analysis used FlowJo.
Celaj S., Gleeson M.W., Deng J., O'Toole G.A., Hampton T.H., Toft M.F., Morrison H.G., Sogin M.L., Putra J., Suriawinata A.A, & Gorham J.D. (2014). The microbiota regulates susceptibility to Fas-mediated acute hepatic injury. Laboratory investigation; a journal of technical methods and pathology, 94(9), 938-949.
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