The animal experiments used in this study were approved by the Ethics Committee of the First Affiliated Hospital of Harbin Medical University, and were carried out under the control of the University's Guidelines for Animal Experimentation. The in vivo implantation surgery process and study methods were reported in detail in our previous investigations.23 (link) In this study, fifteen New Zealand rabbits with weight of 2.5–3 kg each were studied. Then, the Ti, TNZS, SLATi and SLATNZS implants were placed into four machined holes with size of ϕ 2 × 6 mm3 on the tibias of the rabbits. The interfacial bonding status between the implant and bone was evaluated using the X-ray radiographic technique (DHF-155HII, Hitachi, Japan) and micro-computed tomography (Micro-CT, Siemens Co., America), Materialise's interactive medical image control system 20 (MINICS 20, Materialise Co., Belgium), and VG stained cross-sectional histological analysis.
Dhf 155hii
Manufactured by Hitachi
Sourced in Japan
The DHF-155HII is a laboratory centrifuge designed for general-purpose applications. It features a maximum speed of 15,500 RPM and a maximum RCF of 25,300 x g. The centrifuge has a rotor capacity of 6 x 50 mL and can accommodate a variety of sample tube sizes. The DHF-155HII is a reliable and versatile piece of lab equipment for researchers and scientists.
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Lab products found in correlation
4 protocols using dhf 155hii
1
Rabbit Tibia Implant Osseointegration Evaluation
2
Radiological Evaluation of Knee Osteoarthritis
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The radiological evaluation of knee OA was carried out using X‐rays (Hitachi Limited, DHF‐155HII, Tokyo, Japan). Specimens (rabbits) in the two groups were collected at 4 weeks after the injection of collagenase for radiological evaluation. The images of patients were evaluated using the Kellgren–Lawrence (K‐L) grading scale16 . The K‐L scoring criteria use a scale from 0 to 4: 0 = normal; 1 = suspicious narrowing of joint space and possible osteophytic lipping; 2 = definite presence of osteophytes, definite narrowing of joint space; 3 = moderate multiple osteophytes, definite narrowing of joint space, some sclerosis, and possible deformity of the bone contour; 4 = large osteophytes, marked narrowing of joint space, severe sclerosis, and clear deformity of the bone contour. The K‐L score of all the radiographs was read by two blinded expert readers.
3
Radiographic Assessment of Osteoarthritis Progression
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Specimens were collected at 72 h, 1 week, 3 weeks, 6 weeks, and 12 weeks after the surgery or after the last injection. The knee joints were subjected to anteroposterior and lateral X-ray examination (Hitachi Limited, DHF-155HII, Tokyo, Japan). The parameters were set as follows: 42 kV, 250 mA, 32 ms, and 80 cm film-focus distance. The results were evaluated using the Kellgren-Lawrence (K-L) grading scale [18 (link)]. The K-L scoring criteria use a scale from 0 to 4 and is defined as follows: 0 = normal; 1 = questionable narrowing of joint space and possible osteophytic lipping; 2 = definite presence of osteophytes, definite narrowing of joint space; 3 = moderate multiple osteophytes, definite narrowing of joint space, some sclerosis, and possible deformity of the bone contour; 4 = large osteophytes, marked narrowing of joint space, severe sclerosis, and clear deformity of the bone contour.
4
Rabbit Tibia Implant Osseointegration Study
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In this study, all the implantation surgeries fulfilled the requirements of the Animal Care and Experiment Committee. The implantation procedure and analysis approach have already been reported in our previous study.41 Twelve healthy and mature white rabbits (New Zealand, 2.5–3 kg) were employed for the animal investigation. Four and three implant holes (ϕ2 × 6 mm3) were made in the left and right tibias for implanting the Ti, MAO, MAOMH001 and MAOMH3 as well as Ti, MAO and MAOMH05 implants, respectively. The interfacial bonding status was evaluated using X-ray digital photography (DHF-155HII, Hitachi, Japan), micro-computed tomography (Micro-CT, Siemens, Siemens Co., USA), VG stained histological analysis, SEM and EDS analyses, and bone–implant contact (BIC%) calculations. Subsequently, the 3D structures of the tibias containing the samples were rebuilt within the distance range ϕ2 × 6 mm3 to ϕ3 × 6 mm3 using MINICS 20 (Materialise Co., Belgium).
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