α glucosidase g5003

The 2, 2-diphenyl-1-picrylhydrazyl (DPPH), 2, 2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2, 4, 6-Tris(2-pyridyl)-s-triazine (TPTZ), 2, 6-di-tert-butyl-4-methylphenol (BHT), vitamin C (Vc), were obtained from Solarbio Life sciences (Beijing, China). All chemical reagents including methanol, ethyl acetate (EtOAc), petroleum ether (PE), Dichloromethane (DCM), and n-butanol (n-BuOH) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Chromatographic acetonitrile was purchased from Merck (Darmstadt, Germany). Folin-Ciocalteu, pyrogallol, and other analytical grade chemicals were purchased from Aladdin (Shanghai, China). Myricetin, dihydroMyricetin, quercetin, resveratrol, apigenin, baicalein, hesperitin, gallic acid, rutin, luteolin, epicatechin, epicatechin gallate, and other standards for HPLC were purchased from Yuanye Bio-Technology (Shanghai, China). Cellulase (3, 000 μ/g) and pectinase (40, 000 μ/g) were purchased from Xiya reagent (Shandong, China). α-Glucosidase (G5003) and acetylcholinesterase (AChE, C3389) were purchased from Sigma-Aldrich (St. Louis, USA). p-Nitrophenol nitrophenol α-D-glycopyranoside (pNPG), acarbose, galantamine, and cisplatin were purchased from Sigma-Aldrich (St. Louis, MO, USA). The HepG2, MCF-7, Raw264.7, and A549 cells were purchased from Conservation genetics CAS Kunming cell bank.

Lactobacillus Plantarum SWFU D16 was isolated from Yunnan goat milk cake, a Chinese traditional fermented food in our Laboratory. Lactobacillus Plantarum ATCC 8014, Lactobacillus Bulgaricus CICC 6045, Lactobacillus Casei ATCC 334, and Streptococcus Thermophilus CICC 6038 were purchased from the Guangdong Microbial Culture Collection Center (GDMCC), and Lactobacillus Rhamnosus ATCC 53013 was purchased from the American Type Culture Collection (ATCC).
Folin–Ciocalteu reagent, gallic acid, rutin, 2,2-diphenyl-1-picrylhydrazyl radical (DPPH), 2,2’-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), 2,4,6-tri (2-pyridyl)-1,3,5-triazine (TPTZ), iron chloride (FeCl₃6H₂O), p-Nitrophenol α-D-glycopyranoside (pNPG), acetylthiocholine iodide (ATCI), 5,5’-Dithiobis-(2-nitrobenzoic acid) (DTNB), and other chemicals were purchased from Aladdin (Shanghai, China). Chromatographic acetonitrile was purchased from Merck (Darmstadt, Germany). α-Glucosidase (G5003), and acetylcholinesterase (AChE, C3389) were purchased from Sigma-Aldrich (St. Louis, United States). Syringic acid, (+)-catechin, rosmarinic acid, chlorogenic acid, and other standards were purchased from Yuanye Bio-Technology (Shanghai, China).

Fresh GPL was collected from Xishuangbanna, Yunnan Province, China, in January 2020. Lactobacillus plantarum (ATCC 8014), Lactobacillus casei (ATCC 334), and Lactobacillus rhamnosus (ATCC 53013) were purchased from the Guangdong Province General Microbiological Culture Collection Center and the American Microbial Species Preservation Center, respectively. Lactobacillus plantarum (SWFU D16) was isolated by ourselves.
Folin–Ciocalteu reagent, gallic acid, rutin, sodium nitrite (NaNO₂), aluminum nitrate (Al(NO₃)₃), methanol, potassium persulfate, ferrous sulfate (FeSO₄), ferric chloride hexahydrate (FeCl₃·6H₂O), sodium carbonate (Na₂CO₃), trisodium phosphate (Na₃PO₄), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2, 4, 6-tri(2-pyridyl)-s-triazin (TPTZ), and other chemicals were purchased from Aladdin (Shanghai, China). Chromatographic acetonitrile was purchased from Merck (Darmstadt, Germany). P-nitrophenyl-α-D-glucopyranoside (PNPG) and α-glucosidase (G5003) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Catechins, chlorogenic acid, and other standards were purchased from Yuanye Bio-Technology (Shanghai, China).