Bovine serum albumin (BSA) (FUJIFILM Wako Pure Chemical Corporation) was prepared by dissolving in 0.1 mol/l Tris-HCl (FUJIFILM Wako Pure Chemical Corporation) (pH 8.0) to a concentration of 5% and filtered through a 0.22-µm filter for sterilization. Oleic acid (FUJIFILM Wako Pure Chemical Corporation) was dissolved in 5% BSA solution to prepare 4 mM Oleic acid solution. Oleic acid use solution was diluted to 0, 50, 200 and 500 µM with the aforementioned culture medium.
Oleic acid
Manufactured by Fujifilm
Sourced in Japan
Oleic acid is a fatty acid commonly used in laboratory equipment. It is a long-chain monounsaturated carboxylic acid with the molecular formula C18H34O2. Oleic acid serves as a key component in various laboratory applications, providing specific functional properties essential for various experimental processes.
Automatically generated - may contain errors
Lab products found in correlation
Palmitic acid, by Fujifilm (3 mentions)
Methanol, by Fujifilm (2 mentions)
Sodium dodecyl sulfate (sds), by Fujifilm (2 mentions)
Sodium hydroxide (naoh), by Fujifilm (2 mentions)
Ethanol, by Fujifilm (2 mentions)
Microcrystalline cellulose mcc powder, by Merck Group (2 mentions)
Tris hcl, by Fujifilm (1 mentions)
Bovine serum albumin (bsa), by Fujifilm (1 mentions)
Bovine serum albumin bsa, by Fujifilm (1 mentions)
Cholesterol, by Merck Group (1 mentions)
Bovine gall powder, by Fujifilm (1 mentions)
Epigallocatechin gallate hydrate, by Tokyo Chemical Industry (1 mentions)
Lipase, by Merck Group (1 mentions)
Olive oil, by Fujifilm (1 mentions)
Lecithin, by Fujifilm (1 mentions)
Sodium taurocholate, by Fujifilm (1 mentions)
Triolein, by Fujifilm (1 mentions)
Sodium chloride (nacl), by Fujifilm (1 mentions)
Sodium dihydrogen phosphate dihydrate, by Fujifilm (1 mentions)
Caffeine, by Tokyo Chemical Industry (1 mentions)
19 protocols using oleic acid
1
Oleic Acid Preparation and Dilution
2
Investigating Lipid Metabolism Modulation
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Olive oil, bovine gall powder, triolein, lecithin (egg yolk), sodium taurocholate, catechin mixture, and oleic acid were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Lipase, glyceride monooleate, and cholesterol were purchased from Sigma-Aldrich Corporation (Tokyo, Japan). Epigallocatechin gallate hydrate (EGCG) was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). oleic acid-glyceryl monooleate mixture was purchased from MP Biomedicals (Tokyo, Japan). Indigestible dextrin was purchased from Matsutani Chemical Industry Co., Ltd. (Hyogo, Japan). Pectin was purchased from Sansho Co., Ltd. (Osaka, Japan). IMD was produced by Hayashibara Co., Ltd. (Okayama, Japan)[12 (link)].
3
Trospium Chloride and Caffeine Bioavailability
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Trospium chloride and caffeine were purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan). Sodium chloride (NaCl), sodium dihydrogen phosphate dihydrate (NaH2PO4 2H2O), 8N NaOH, taurocholic acid (TC), and oleic acid (OA) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Propantheline bromide was purchased from Funakoshi Co., Ltd (Tokyo, Japan). Ambenonium dichloride was purchased from Toronto Research Chemicals (Ontario, Canada). Egg yolk lecithin (EL) was purchased from NOF Corporation (Tokyo, Japan). Glyceryl mono-oleate (GM) was purchased from Nippon Surfactant Industries Co., Ltd (Tokyo, Japan). A cellulose dialysis membrane was purchased from As-One Corporation (Osaka, Japan).
The Food and Drug Adminstration (FDA) breakfast ingredients were purchased from the local market (bacon (Itoham Foods Inc, Japan), toast (Choujuku bread, Pasco Shikishima Corporation, Japan), egg (Odama mix, I.T.S Farm Co., Ltd, Japan), hash browns (Hoshino potato, Heinz Japan Ltd, Japan), whole milk (Oishii Megumilk Snow Brand Milk, MEGMILK SNOW BRAND Co., Ltd, Japan), and butter (Hokkaido-butter, MEGMILK SNOW BRAND Co., Ltd, Japan)).
The Food and Drug Adminstration (FDA) breakfast ingredients were purchased from the local market (bacon (Itoham Foods Inc, Japan), toast (Choujuku bread, Pasco Shikishima Corporation, Japan), egg (Odama mix, I.T.S Farm Co., Ltd, Japan), hash browns (Hoshino potato, Heinz Japan Ltd, Japan), whole milk (Oishii Megumilk Snow Brand Milk, MEGMILK SNOW BRAND Co., Ltd, Japan), and butter (Hokkaido-butter, MEGMILK SNOW BRAND Co., Ltd, Japan)).
4
Skin Permeation Study of Antimicrobial Compounds
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ACV and IPM were obtained from Tokyo Chemical Industries Co. Ltd. (Tokyo, Japan). Choline chloride ([Ch][Cl]), silver oxide (Ag2O), lactic acid, oleic acid, methanol, and acetonitrile were purchased from Wako Pure Chemical Industries Ltd. (Osaka, Japan). Formic acid and propionic acid were obtained from Kishida Chemical Co., Ltd., (Osaka, Japan). Tween-80 and Span-20 were procured from Sigma–Aldrich Chemical Co., (St. Louis, MO, USA). The skin of a female Yucatan micropig (YMP) was received from Charles River Japan Inc., Yokohama, Japan.
The three-dimensional cultured human epidermis model (LabCyte EPI-Model 12) was supplied by Japan Tissue Engineering Co., Ltd., Gamagori Miyakitadori, Aichi, Japan. 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was obtained from Dojindo Molecular Technologies, Inc., Kumamoto, Japan. All other chemicals and solvents used in the experiments were of analytical grade.
The three-dimensional cultured human epidermis model (LabCyte EPI-Model 12) was supplied by Japan Tissue Engineering Co., Ltd., Gamagori Miyakitadori, Aichi, Japan. 3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) was obtained from Dojindo Molecular Technologies, Inc., Kumamoto, Japan. All other chemicals and solvents used in the experiments were of analytical grade.
5
Preparation and Analysis of Canola Oil
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Canola oil was manufactured by J-OIL MILLS, Inc. (Tokyo, Japan; Supplementary Table S1 ). Aquamicron AX and Aquamicron CXU were purchased from Mitsubishi Chemical Corporation (Tokyo, Japan). ADAM reagent was obtained from Funakoshi Co., Ltd. (Tokyo, Japan). Oleic acid, dimethyl nonanedioate, and methyl heptadecanoate were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan). Undecanoic acid was purchased from Tokyo Chemical Industry Co., Ltd. (Tokyo, Japan). Azelaic acid was obtained from Sigma-Aldrich (St. Louis, U.S.A.). Leucine-enkephalin was obtained from Waters (Milford, MA, U.S.A.). Frozen French fries were purchased from a market in Kanagawa, Japan. Other reagents were of the highest grade available.
6
Steatosis induction in Hepa1-6 cells
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The Hepa1-6 cell line, which is derived from a BW7756 tumor from a C57BL/6J mouse, was purchased from DS Pharma Biomedical Japan (Osaka, Japan). To observe the effect of steatosis in hepatocytes, Hepa1-6 cells were exposed to 0.1 mM oleic acid or palmitic acid (Wako Chemical, Osaka, Japan) for 3 h, as described previously [21 (link)]. Lipid accumulation was confirmed by Sudan III staining [21 (link)]. The concentrations of CCL2 present in culture supernatants were estimated by ELISAs.
7
Dinapinones Purification and Radiolabeled Lipid Assays
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All dinapinones were purified from a culture broth of the microorganism T. pinophilus FKI-3864 according to established methods19 (link),21 (link). Vioxanthin was kindly gifted from Prof. Michael Muller at Institut für Biotechnologie 2, Jülich, Germany. [1-14C]oleic acid (1.85 GBq/mmol) and [14C]oleoyl-CoA (1.85 GBq/mmol) were purchased from PerkinElmer (Waltham, MA, U.S.A.). [1-14C]palmitoyl-CoA was purchased from Moravek Biochemicals (Brea, CA, U.S.A.). Phosphatidic acid, L-a-dioleoyl-[2-oleoyl-1-14C] (2.04 GBq/mmol) and glycerol, L-[14C(U)] 3-phosphate (5.55 GBq/mmol) were purchased from American Radiolabeled Chemicals (St. Louis, MO, U.S.A.). Fetal bovine serum (FBS) was purchased from Biowest (Nuaille, France). Penicillin (10,000 units/ml) and streptomycin (10,000 mg/ml) were purchased from Invitrogen (Carlsbad, CA, U.S.A.). Ham’s F-12 medium, G3P, BSA, 1,2-dioleoyl-sn-glycerol, palmitoyl-CoA, Triton X-100, poly L-lysine, oil red O, bafilomycin A1, 3-(4,5-dimethylthiazo-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT), isoproterenol and rapamycin were purchased from Sigma-Aldrich (St. Louis, MO, U.S.A.). DMEM (high glucose) medium, diisopropyl fluorophosphates, 2-mercaptoethanol, oleic acid and hematoxylin were purchased from Wako (Osaka, Japan). Nonidet P-40 was purchased from Nacalai Tesque (Kyoto, Japan).
8
Influenza HA Vaccine Preparation
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Sodium dodecyl sulfate (SDS), sodium laureth sulfate (LES) and oleic acid were purchased from Wako Pure Chemical Industries (Osaka, Japan), NOF Corporation (Tokyo, Japan) and Tokyo Chemical Industry (Tokyo, Japan), respectively. Potassium hydroxide was purchased from Wako Pure Chemical Industries. All reagents were used without further purification.
An influenza HA vaccine for the 2013–2014 season (manufacturing number HA130A), containing the HA proteins of A/California/7/2009(H1N1) pdm09, A/Texas/50/2012(H3N2) and B/Massachusetts/2/2012, was purchased from Biken Corporation Ltd. (Osaka, Japan). The vaccine was a split vaccine made from purified virus particles and contained more than 90 µg/ml of the HA proteins from the three viruses.
An influenza HA vaccine for the 2013–2014 season (manufacturing number HA130A), containing the HA proteins of A/California/7/2009(H1N1) pdm09, A/Texas/50/2012(H3N2) and B/Massachusetts/2/2012, was purchased from Biken Corporation Ltd. (Osaka, Japan). The vaccine was a split vaccine made from purified virus particles and contained more than 90 µg/ml of the HA proteins from the three viruses.
9
Preparation of Artificially Soiled Fabric
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The materials used to prepare the artificially soiled fabric were cotton canequim (Laundry Science Association in Japan) , oleic acid (Fujifilm Wako Chemicals) , palmitic acid (Fujifilm Wako Chemicals) , and Sudan IV (Fujifilm Wako Chemicals) . Sodium hydroxide (Fujifilm Wako Chemicals, guaranteed grade) was used to adjust the pH of the cleaning solution, and sodium dodecyl sulfate (SDS, Fujifilm Wako Chemicals, for Biochemistry) was used as a surfactant.
10
Bisacurone Inhibits Lipid Accumulation
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Bisacurone was purchased from Nagara Science Co., Ltd. (Gifu, Japan). Curcumin, palmitic acid, oleic acid, and ImmunoStar LD was purchased from FUJIFILM Wako Pure Chemical Industries (Osaka, Japan). Their chemical structures are shown in Fig. 1 . Metformin hydrochloride was from Sigma-Aldrich (St. Louis, MO). Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Nissui Pharmaceutical (Tokyo, Japan). Bovine serum albumin (BSA), Blocking One, and Blocking One-P solutions were from Nacalai Tesque, Inc. (Kyoto, Japan). For western blotting, antibodies for lamin B, PPARα, CPT1, fatty acid binding protein 4 (FABP4), C/EBPα, FAS, anti-mouse IgG, anti-goat IgG, and anti-rabbit IgG were purchased from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Santa Cruz, CA); β-actin, p-AMPKα, AMPKα, p-LKB1, and LKB1 were from Cell Signaling Technology (Beverly, MA); ChREBP and SREBP-1 were from Abcam (Cambridge, MA). The polyvinylidene difluoride membrane was products of GE Healthcare Bio-Science Co., (Piscataway, NJ). All other reagents used were of the highest grade available from commercial sources.
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