Lsm 880 with fast airyscan

Manufactured by Zeiss

Sourced in Germany

The Zeiss LSM 880 with Fast Airyscan is a high-resolution confocal laser scanning microscope. It features the Fast Airyscan detector, which enables improved image quality and increased acquisition speed compared to conventional confocal microscopes.

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Lab products found in correlation

Zen black software, by Zeiss (1 mentions) Fish tag dna multicolor kit, by Thermo Fisher Scientific (1 mentions) Lsrfortessa flow cytometer, by BD (1 mentions) Ab150078, by Abcam (1 mentions)

Spelling variants of this product

LSM 880 (3798 citations) Airyscan (486 citations) LSM 880 Airyscan (196 citations) 880 Airyscan (36 citations) Fast Airyscan (27 citations) LSM 880 with Airyscan (17 citations)

4 protocols using lsm 880 with fast airyscan

Chromosome 4 miRNA Locus Detection

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Probes for DNA-FISH were prepared using the FISH Tag™ DNA Multicolor Kit (Thermo Fisher) following kit instructions. The BAC DKEY-69C19 was used as a template for DNA-FISH probe production, corresponding to 214 kb of the mir430 locus on chromosome 4.
Embryos were fixed at the developmental stage of interest using 4% PFA. Animal caps were isolated and a series of gradient washes was used to equilibrate the samples with hybridization buffer [50% formamide, 4x SSC, 100 mM NaPO4 pH 7.0, 0.1% Tween-20]. Embryonic DNA was denatured by incubation of samples at 70 °C for 15 min before application of probe and incubation overnight at 37 °C. Unbound probe was washed from the samples using a hybridization buffer: PBS-T gradient. Samples were counterstained with DAPI prior to imaging on a Zeiss LSM 880 with Fast Airyscan, with a 100 × 1.48 numerical aperture objective lens. 50–100 optical sections (130 nm thickness) were acquired of each nucleus. Acquired images were processed using Zen Black software (Zeiss).

Hadzhiev Y., Qureshi H.K., Wheatley L., Cooper L., Jasiulewicz A., Van Nguyen H., Wragg J.W., Poovathumkadavil D., Conic S., Bajan S., Sik A., Hutvàgner G., Tora L., Gambus A., Fossey J.S, & Müller F. (2019). A cell cycle-coordinated Polymerase II transcription compartment encompasses gene expression before global genome activation. Nature Communications, 10, 691.

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Mitochondrial Membrane Potential Assay

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Mitochondrial membrane potential was measured using JC-1 probe. For flow cytometric analysis of JC-1 Staining, U87 MG and U251 cells were cultured in 6-well-plates and treated with different concentrations (0, 40, and 80 μM) of kaempferol for 24 h. Then, the cells were collected and stained with 500 μL 1 × JC-1 staining solution and 500 μL of DMEM for 30 min at room temperature, and the stained cells were tested using a BD LSRFortessa flow cytometer with emission at 590 and 529nm. JC-1 accumulates as J-aggregates (590 nm; red) only in metabolically active mitochondria and depolarization of mitochondrial membranes leads to JC-1 monomer formation (527 nm; green). For fluorescence image assay, U87 MG and U251 cells were cultured in cover glass bottom dishes and treated with 80 μM of kaempferol for 24 h. After stimulation, the cells were stained with JC-1 staining solution (1×) for 30 min and then observed and photographed using a Confocal microscope (LSM 880 with fast Airyscan, Zeiss, Germany).

Chen S., Ma J., Yang L., Teng M., Lai Z.Q., Chen X, & He J. (2020). Anti-glioblastoma Activity of Kaempferol via Programmed Cell Death Induction: Involvement of Autophagy and Pyroptosis. Frontiers in Bioengineering and Biotechnology, 8, 614419.

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Rociletinib-induced ABCG2 Internalization

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Immunofluorescence confocal microscopy was used to examine whether treatment of rociletinib leads to internalization of ABCG2 as the previously described³¹ (link) with slight modifications. In brief, S1-MI-80 cells (3 × 10⁴) were seeded on 13 mm square glass cover slides in 24-well plates, treated with 1 μmol/L rociletinib, then incubated at 4 °C for 48 h. The cells were washed with PBS, and then fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.2% Triton X-100 for 10 min. After washing three times with PBS, cells were blocked with 5% BSA for 45 min, and incubated with anti-ABCG2 primary antibody (1:100; Cat#ABP0148; Abbkine, CA, USA) overnight at 4 °C. Then stained with an Alexa Fluor 488-conjugated anti-rabbit IgG F (ab')2 fragment (1:100; Cat#4412; CST, Beverly, MA, USA) at room temperature in dark for 1 h. Nuclei was stained with 4′,6-diamidino-2-phenylindole (DAPI, 2.5 μg/mL) for 5 min and cells were observed and imaged using confocal microscope (LSM 880 with fast AirysCan; Carl Zeiss, Jena, Germany).

Zeng F., Wang F., Zheng Z., Chen Z., Wah To K.K., Zhang H., Han Q, & Fu L. (2020). Rociletinib (CO-1686) enhanced the efficacy of chemotherapeutic agents in ABCG2-overexpressing cancer cells in vitro and in vivo. Acta Pharmaceutica Sinica. B, 10(5), 799-811.

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Confocal Imaging of β-Catenin in GBC Cells

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The GBC cells were seeded in 15 mm confocal dishes (NEST Biotech., Jiangsu, China) and incubated for 24 hours before staining. The cells were fixed in 4% paraformaldehyde and then permeabilized in 0.1% Triton X-100 at room temperature. After being blocked for 1 h with phosphate-buffered saline/Tween (PBST) containing 10% goat serum, the cells were incubated with β-catenin primary antibodies (ab32572; 1:200) overnight at 4 ℃. After 3 washes with PBST, a cocktail of fluorescence-conjugated secondary antibody (ab150078, 1:100, Abcam, Cambridge, MA, USA) was added to the cells. Then, the cell nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI; C0065, Solarbio Corp., China), and the cells were photographed under a confocal microscope (LSM880 with Fast Airyscan; ZEISS, Oberkochen, Germany).

Zhu H., Chen Z., Yu J., Wu J., Zhuo X., Chen Q., Liang Y., Li G, & Wan Y. (2022). MiR-195-5p suppresses the proliferation, migration, and invasion of gallbladder cancer cells by targeting FOSL1 and regulating the Wnt/β-catenin pathway. Annals of Translational Medicine, 10(16), 893.

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