Total nucleic acid was extracted from the whole blood using the QIAsymphony SP platform using the Virus Blood 200 protocol (Qiagen, Hilden, Germany) and cell preparations were extracted using the NucliSENS easyMAG platform (bioMerieux, Boxtel, the Netherlands) with final elution volume of 50 μL. For HHV6b detection, primers for HHV6 UL38 gene (forward [5’-GGA GTG CCT GTG GGT ATT C-3’], reverse [5’-CTA AGG TGA GCC AGA TTC G-3’]) and FAM/ZEN/Iowa Black quencher-labeled TaqMan probe were used (Applied Biosystems, Foster City, CA, USA). Quantitative PCR was performed using an ABI 7500 Fast real-time PCR machine with standard cycling protocol (Applied Biosystems).
Fam zen iowa black quencher labeled taqman probe
Manufactured by Thermo Fisher Scientific
Sourced in United States
The FAM/ZEN/Iowa Black quencher-labeled TaqMan probe is a fluorescently-labeled oligonucleotide designed for use in TaqMan assays. The probe features a FAM reporter dye at the 5' end, a ZEN internal quencher, and an Iowa Black quencher at the 3' end. This configuration allows for improved signal-to-noise ratio and enhanced detection sensitivity in qPCR applications.
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2 protocols using fam zen iowa black quencher labeled taqman probe
1
Quantitative PCR for HHV6b Detection
2
Quantitative PCR for HHV6b Detection
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Total nucleic acid was extracted from the whole blood using the QIAsymphony SP platform using the Virus Blood 200 protocol (Qiagen, Hilden, Germany) and cell preparations were extracted using the NucliSENS easyMAG platform (bioMerieux, Boxtel, the Netherlands) with final elution volume of 50 μL. For HHV6b detection, primers for HHV6 UL38 gene (forward [5’-GGA GTG CCT GTG GGT ATT C-3’], reverse [5’-CTA AGG TGA GCC AGA TTC G-3’]) and FAM/ZEN/Iowa Black quencher-labeled TaqMan probe were used (Applied Biosystems, Foster City, CA, USA). Quantitative PCR was performed using an ABI 7500 Fast real-time PCR machine with standard cycling protocol (Applied Biosystems).
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