Myricetin (DY0103, HPLC ≥98%) was purchased from MUST Biotechnology Co., Ltd. (Chengdu, China) for animal study. For cell experiments, Myricetin (70050) and DMSO (D2650) was obtained from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle medium (DMEM) and horse serum (16050130) were bought from Gibco (Carlsbad, CA). Fetal bovine serum (FBS) was purchased from Hyclone Laboratories, Inc. (Logan, UT, USA). Cell Counting Kit-8 (CCK-8) was purchased from Dojindo (Kumamoto, Japan). mirVana™ miRNA Inhibitors (rno-miR-499-5p, MH11352), mirVanaTM miRNA mimics (rno-miR-499-5p, MC11352), Lipofectamine RNAiMAX Transfection Reagent (13778083) and antibody against PGC-1α (PA5–38021) were bought from Invitrogen (Massachusetts, USA). Antibodies against slow skeletal myosin heavy chain (ab11083), fast skeletal myosin heavy chain (ab91506), Sox6 (ab30455), tnni1 (ab231720) and myoglobin (ab77232) were purchased from Abcam (Cambridge, UK). Antibody against Cytochrome C (Cyt C, sc-13,561) and β-actin (sc-47778) were purchased from Santa Cruz Biotechnology (CA, USA).
Ab231720
Manufactured by Abcam
Sourced in United Kingdom, United States
Ab231720 is a laboratory reagent. It is a recombinant protein that can be used in various research applications. The core function of this product is to provide a specific biological molecule for experimental purposes. No further details on intended use or interpretation are provided.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay kit, by Thermo Fisher Scientific (2 mentions)
Ab30455, by Abcam (1 mentions)
Ab77232, by Abcam (1 mentions)
Ab91506, by Abcam (1 mentions)
Ab11083, by Abcam (1 mentions)
Horse serum, by Thermo Fisher Scientific (1 mentions)
Mirvana mirna inhibitor, by Thermo Fisher Scientific (1 mentions)
Lipofectamine rnaimax transfection reagent, by Thermo Fisher Scientific (1 mentions)
Dulbecco s modified eagle medium dmem, by Thermo Fisher Scientific (1 mentions)
β actin sc 47778, by Santa Cruz Biotechnology (1 mentions)
Fetal bovine serum (fbs), by Cytiva (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Myricetin, by Merck Group (1 mentions)
Ab205719, by Abcam (1 mentions)
Gapdh antibody, by Cell Signaling Technology (1 mentions)
Ab154711, by Abcam (1 mentions)
Ab7090, by Abcam (1 mentions)
Odyssey ir scanner, by LI COR (1 mentions)
Ripa lysis buffer, by Beyotime (1 mentions)
2 protocols using ab231720
1
Myricetin Modulates Skeletal Muscle Metabolism
2
Gastrocnemius Muscle Protein Analysis
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The gastrocnemius muscle tissues were lysed using ice-cold RIPA lysis buffer (Beyotime Biotech, Shanghai, China) supplemented with 1% phenylmethylsulfonyl fluoride (PMSF), and then centrifuged at 12 000 rpm for 15 min at 4°C. Then, the protein concentration was quantified using a bicinchoninic acid (BCA) protein assay kit (Thermo Scientific, USA). We used primary antibodies against Rac2 (#ab154711, 1: 2000; Abcam, Cambridge, MA), Itgb2 (#73663, 1: 1000; Cell Signaling), Lcp2 (#4958, 1: 1000; Cell Signaling), Myl3 (#ab680, 1: 100; Abcam, Cambridge, MA), Tnni1 (#ab231720, 1: 2000; Abcam, Cambridge, MA), Toll-like receptor 4 (TLR-4, #14358, 1: 1000; Cell Signaling), and NF-κB p65 (#8242, 1: 1000; Cell Signaling), followed by incubating with the secondary antibody against IgG (#ab7090 or #ab205719, 1: 5000, Abcam, Cambridge, USA). Protein levels were normalized by probing the same blots with a GAPDH antibody (#3683, 1: 1,000; Cell Signaling). Finally, the protein bands were visualized with an Odyssey® IR scanner (LI-COR, USA) and quantified by Image J (version 1.8.0) software (National Institutes of Health, NY).
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