Perchloric acid

Perchloric acid was obtained from Loba Chemie (Mumbai, India). The standard of biogenic amines, putrescine dihydrochloride and spermidine trihydrochloride, were obtained from Sigma-Aldrich (St. Louis, MO, USA). Water, methanol, and acetonitrile of HPLC grade were obtained from RCl Labscan (Bangkok, Thailand). The other reagents for synthesis were: sodium acetate anhydrous, purchased from QReC (Selangor, Malaysia); sodium 1-octanesulfonate monohydrate, purchased from Sigma-Aldrich (St. Louis, MO, USA); boric acid and glacial acetic acid, obtained from RCl Labscan (Bangkok, Thailand); potassium hydroxide, purchased from Kemaus (Cherrybrook, New South Wales, Australia); Brij 35, purchased from Merck (Billerica, MA, USA); and 2-mercaptoethanol, purchased from Merck (Darmstadt, Germany). OPA was obtained from Himedia (Mumbai, India).

Doxorubicin (2 mg/mL) [Hikma Specialized Pharmaceuticals, Cairo] and Rifactine (300 mg rifampicin) [MUP (Medical Union Pharmaceuticals), Giza, Cairo, Egypt] were used. Meanwhile, nitro blue tetrazolium (≥ 90%), nicotinamide adenine dinucleotide reduced form (NADH, ≥ 97%), sodium pyrophosphate tetrabasic (≥ 95%), phenazine methosulfate (≥ 90%), trichloroacetic acid (≥ 99%), and thiobarbituric acid (≥ 98%) were obtained from Sigma-Aldrich, Saint Louis, MO, United States. Perchloric acid (70%) was bought from LOBA CHEMIE PVT.LTD, Mumbai, Maharashtra, India. Absolute ethanol (99.9%) was acquired from Alfa Chemical Group, Hadayek El-Kobba, Cairo, Egypt. In addition, potassium dihydrogen orthophosphate (98–100%) and sodium chloride saline solution (0.9%) were purchased from ELNASR PHARMACEUTICAL CHEMICALS, Qalyubia, Egypt. Di-potassium hydrogen phosphate (99%) was bought from HiMedia Laboratories C40, Maharashtra, India. All other solvents and chemicals obtained for the experiments were of the highest available purity and were used as received.