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Murex htlv 1 2

Manufactured by DiaSorin
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Sourced in United Kingdom, Italy, China, Germany

The Murex HTLV-I+II is a laboratory equipment product manufactured by DiaSorin. It is designed to detect the presence of antibodies to the human T-cell lymphotropic virus types I and II (HTLV-I and HTLV-II) in human serum or plasma samples.

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16 protocols using murex htlv 1 2

1

Comparative Evaluation of HTLV-1/2 ELISA Assays

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Three different kinds of commercially available ELISA assays [Wantai HTLV-1/2 antibody ELISA kits (Wantai BioPharm, China), Murex HTLV I+II (Diasorin S.p.A., United Kingdom) and Foresight HTLV-1/2 antibody ELISA kits (Acon Biotech, China)] were used for screening HTLV1/2 antibodies at all participating blood centers/banks in China. Reactive specimens were retested by the same assay and any one of two-round retests found to be reactive were defined as RR specimen.
Further detection was done in the NCCL using Elecsys HTLV-I/II (Roche Diagnostics, Germany), Lumipulse G HTLV-I/II (Fujirebio, Japan) and two kinds of ELISA assays (Murex HTLV I+II (Diasorin S.p.A., United Kingdom) and Avioq HTLV-I/II Microelisa System (Avioq, Durham, NC, United State). The key features of the evaluated assays are summarized in Table 1.
Ji H., Chang L., Yan Y., Jiang X., Sun H., Guo F, & Wang L. (2020). A Strategy for Screening and Confirmation of HTLV-1/2 Infections in Low-Endemic Areas. Frontiers in Microbiology, 11, 1151.
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2

HTLV-1/2 Antibody Screening and Confirmation Protocol

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Samples were initially screened for HTLV1/2 antibodies using one of the following three commercially available ELISA assays, including Wantai HTLV-1/2 antibody ELISA kits (Wantai BioPharm, China), Murex HTLV I+II (Diasorin S.p.A., United Kingdom), and Foresight HTLV-1/2 antibody ELISA kits (Acon Biotech, China). Reactive specimens underwent retests by the same assay, and any one of the two-round retests that were reactive would be defined as an initially anti-HTLV-1/2 reactive specimen and was sent to the NCCL for further confirmation.
Two different serologic assays, namely, Elecsys HTLV-I/II (Roche Diagnostics, Germany) and Murex HTLV I+II (Diasorin S.p.A., UK), were performed on one sample simultaneously in NCCL. Any sample that reacted to one of the two assays was confirmed and discriminated by the line immunoassay (INNO-LIA HTLV I/II Score, Fujirebio, Japan).
Ji H., Chang L., Yan Y., Sun H., Liu Y, & Wang L. (2023). Genetic typing and intrafamilial transmission of human T-lymphotropic virus type 1 in non-endemic areas of China. Frontiers in Microbiology, 14, 1288990.
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3

Screening for HTLV-1/2 Antibodies in Brazil

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The samples from São Paulo were screened for the presence of HTLV-1/2 antibodies by two enzyme immunoassays (EIAs): Murex HTLV-I+II (DiaSorin S.p.A., Dartford, UK) and Gold ELISA (enzyme-linked immunosorbent assay) HTLV-1/2 (REM Indústria e Comércio Ltd., São Paulo, Brazil). The samples from Salvador were initially screened by the Ortho HTLV-1/HTLV-2 Ab-Capture ELISA system (Ortho Clinical Diagnostics, Raritan, NJ, USA) as well as by four other HTLV-1/2 screening tests commercially available in Brazil: three EIAs (Murex HTLV-I+II [DiaSorin S.p.A., Dartford, UK], anti-HTLV-1/2 Sym solution [Symbiosis Diagnóstica Ltd., Leme, Brazil], and Gold ELISA HTLV-1/2 [REM Indústria e Comércio Ltd., São Paulo, Brazil]) and one chemiluminescence assay (CLIA) kit (Architect rHTLV-1/2; Abbott Diagnostics Division, Wiesbaden, Germany). All assays were performed according to the manufacturers’ instructions, which were also used to interpret results. Cutoff values and gray zones were calculated for each assay, and samples considered reactive or inconclusive in screening were submitted for a confirmatory assay.
Campos K.R., Santos F.L., da Silva Brito V., Gonçalves N.L., Araujo T.H., Galvão-Castro B, & Caterino-de-Araujo A. (2019). Line Immunoassay for Confirmation and Discrimination of Human T-Cell Lymphotropic Virus Infections in Inconclusive Western Blot Serum Samples from Brazil. Journal of Clinical Microbiology, 58(1), e01384-19.
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4

HTLV-1/2 Antibody Screening and Confirmation Protocol

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Samples were initially screened for HTLV1/2 antibodies using one of the following three commercially available ELISA assays, including Wantai HTLV-1/2 antibody ELISA kits (Wantai BioPharm, China), Murex HTLV I+II (Diasorin S.p.A., United Kingdom), and Foresight HTLV-1/2 antibody ELISA kits (Acon Biotech, China). Reactive specimens underwent retests by the same assay, and any one of the two-round retests that were reactive would be defined as an initially anti-HTLV-1/2 reactive specimen and was sent to the NCCL for further confirmation.
Two different serologic assays, namely, Elecsys HTLV-I/II (Roche Diagnostics, Germany) and Murex HTLV I+II (Diasorin S.p.A., UK), were performed on one sample simultaneously in NCCL. Any sample that reacted to one of the two assays was confirmed and discriminated by the line immunoassay (INNO-LIA HTLV I/II Score, Fujirebio, Japan).
Ji H., Chang L., Yan Y., Sun H., Liu Y, & Wang L. (2023). Genetic typing and intrafamilial transmission of human T-lymphotropic virus type 1 in non-endemic areas of China. Frontiers in Microbiology, 14, 1288990.
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5

Serological and Molecular Detection of HTLV-1/2

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Immunoenzymatic assays (Murex HTLV-I+II, DiaSorin, Dartford, UK) were used to detect anti-HTLV-1/2 antibodies in the tested samples following the manufacturer's instructions. Samples with positive results were subjected to line immunoassays (INNO-LIA® HTLV I/II Score, Fujirebio, Japan) and real-time PCR (Applied Biosystems Step One Plus Real Time PCR) to confirm infection and differentiate the viral type, respectively.
Brito W.R., Cardoso-Costa G.D., Roland Junior L.M., Pereira K.A., Lopes F.T., dos Santos B.C., de Lima A.C., Abreu I.N., Lima C.N., Lima S.S., Cayres Vallinoto I.M., dos Santos E.J., Guerreiro J.F, & Vallinoto A.C. (2022). Prevalence and Risk Factors for HTLV-1/2 Infection in Quilombo Remnant Communities Living in the Brazilian Amazon. Frontiers in Public Health, 10, 871865.
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6

HTLV-1/2 Antibody Screening by ELISA

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The samples were submitted to serological screening for the detection of anti-HTLV-1 and anti-HTLV-2 antibodies using an enzyme-linked immunosorbent assay (ELISA; Murex HTLV-I+II, DiaSorin, Dartford, UK).
Abreu I.N., Lopes F.T., Lima C.N., Barbosa A.D., de Oliveira L.R., Fujishima M.A., Freitas F.B., dos Santos M.B., de Lima V.N., Cayres-Vallinoto I.M., Castelo-Branco S., da Silva H.P, & Vallinoto A.C. (2022). HTLV-1 and HTLV-2 Infection Among Warao Indigenous Refugees in the Brazilian Amazon: Challenges for Public Health in Times of Increasing Migration. Frontiers in Public Health, 10, 833169.
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7

Serological Screening for HTLV-1/2 Infection

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Of the 537 international migrants who consented to participate, a total of 528 (98.3%) serum samples were tested for anti-HTLV-1/2 (Murex HTLV-I + II, DiaSorin, Dartford, UK) using an enzyme-linked immunosorbent assay (ELISA). Reactive samples were confirmed using a line immunoassay (INNO-LIA HTLV I/II, Fujirebio, Europe N.V., Belgium). Samples that tested positive by LIA were considered positive for HTLV-1 or 2 infections.
Marinho T.A., Magalhães L.S., dos Santos K.C., Martins T.L., Silva G.R., Silva Á.M., Carneiro M.A., Caetano K.A., Teles S.A, & Martins R.M. (2023). Human T-lymphotropic virus 1/2 infection among immigrants and refugees in Central Brazil, an emerging vulnerable population. Frontiers in Public Health, 11, 1265100.
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8

HTLV-1/HTLV-2 Serological and Molecular Screening

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Peripheral blood (10 ml) was collected from each participant in a tube containing ethylenediaminetetraacetic acid (EDTA) as an anticoagulant. The samples were transported to the Virology Laboratory of the Biological Sciences Institute, Federal University of Pará. Plasma and formed elements were separated by centrifugation at 8,944 g for 15 min, transferred to cryotubes, and frozen at −20°C until serological and molecular analyses.
Plasma samples were screened for anti-HTLV-1/HTLV-2 antibodies using a qualitative enzyme-linked immunosorbent assay (ELISA; Murex HTLV I + II; DiaSorin, Saluggia, Italy) in the Virology Section of Evandro Chagas Institute/Health Surveillance Secretariat, Ministry of Health of Brazil. Reactive or inconclusive results were confirmed using Real-time PCR (Applied Biosystems, Foster City, CA, United States), which also differentiated between HTLV-1 and HTLV-2 infection.
Alencar S.P., Souza M.D., Fonseca R.R., Menezes C.R., Azevedo V.N., Ribeiro A.L., Lima S.S., Laurentino R.V., Barbosa M.D., Freitas F.B., Oliveira-Filho A.B, & Machado L.F. (2020). Prevalence and Molecular Epidemiology of Human T-Lymphotropic Virus (HTLV) Infection in People Living With HIV/AIDS in the Pará State, Amazon Region of Brazil. Frontiers in Microbiology, 11, 572381.
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9

ELISA for Anti-HTLV-1/2 Antibody Detection

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To detect total anti-HTLV-1/2 antibodies, plasma samples were tested in duplicate using an enzyme-linked immunosorbent assay (ELISA) (MUREX HTLV-I + II, DiaSorin, Dartford, UK) following the protocol from the manufacturer.
Abreu I.N., Lima C.N., Sacuena E.R., Lopes F.T., da Silva Torres M.K., dos Santos B.C., de Oliveira Freitas V., de Figueiredo L.G., Pereira K.A., de Lima A.C., Brito W.R., Botelho B.J., Gonçalves J.S., Lima S.S., Vallinoto I.M., Guerreiro J.F., Ishak R, & Vallinoto A.C. (2022). HTLV-1/2 in Indigenous Peoples of the Brazilian Amazon: Seroprevalence, Molecular Characterization and Sociobehavioral Factors Related to Risk of Infection. Viruses, 15(1), 22.
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10

HTLV-1/2 Antibody Detection Protocol

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The detection of anti-HTLV-1/2 antibodies was performed using an ELISA kit (Murex HTLV-I+II, DiaSorin, Dartford, UK) following the protocol suggested by the manufacturer. All samples considered reactive or indeterminate in this test were subjected to confirmatory tests (INNO-LIA and/or real-time PCR).
de Lima A.C., Lopes F.T., de Oliveira Freitas V., Assad M.N., de Sousa R.S., Gonçalves J.S., Gomes J.L., dos Santos B.C., Lima C.N., Abreu I.N., dos Santos Brito W.R., Pereira K.A., da Silva Torres M.K., Lima S.S., Aben-Athar C.Y., Guerreiro J.F., Cayres Vallinoto I.M., Vallinoto A.C, & Feitosa R.N. (2022). Prevalence and Risk Factors for HTLV-1/2 Infection inRiverside and Rural Populations of the State of Pará. Viruses, 14(10), 2262.
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