SP was obtained from GenScript (Piscataway, NJ) and dissolved in 1× PBS. The NK-1R antagonist L733060 and the dual NK-1R/Mrgprs antagonist QWF (Boc-Gln-D-Trp[formyl]-Phe benzyl ester trifluoroacetate) were obtained from Sigma (St Louis, Mo). QWF and L733060 were dissolved in dimethyl sulfoxide (DMSO; Sigma) at 10 mmol/L and diluted in PBS for use at concentrations of 500 µmol/L or less. In the nociceptive studies with Capsaicin, in which QWF was diluted in DMSO, the final concentration of DMSO was 0.01%. Capsaicin was obtained from Tocris Bioscience (Bristol, United Kingdom).
L 733 060
Manufactured by Merck Group
Sourced in United States
L-733,060 is a laboratory product manufactured by Merck Group. It is a chemical compound used in various research and scientific applications. The core function of L-733,060 is to serve as a research tool, without further elaboration on its intended use.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco s modified eagle medium dmem buffer, by Thermo Fisher Scientific (2 mentions)
4 2 hydroxyethyl 1 piperazineethanesulfonic acid hepes buffer, by Thermo Fisher Scientific (2 mentions)
Hank s balanced salt solution hbss buffer, by Thermo Fisher Scientific (2 mentions)
S way 100635, by Merck Group (2 mentions)
S6883, by Merck Group (1 mentions)
C4382, by Merck Group (1 mentions)
L 733 060, by Bio-Techne (1 mentions)
Clozapine, by Merck Group (1 mentions)
Acetylcholine chloride, by Merck Group (1 mentions)
Dopamine, by Merck Group (1 mentions)
Serotonin, by Merck Group (1 mentions)
3 isobutyl 1 methylxanthine ibmx, by Merck Group (1 mentions)
Capsaicin, by Bio-Techne (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
B ba f3, by Eurofins (1 mentions)
Butaclamol, by Merck Group (1 mentions)
5 protocols using l 733 060
1
Nociceptive Signaling Antagonist Protocols
2
Mice Model for Ophthalmic Research
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Six-week-old male Balb/c mice were obtained from Chiyoda Kaihatsu (Tokyo, Japan). Animals were treated in accordance with the ARVO Statement for the Use of Animals in Ophthalmic and Vision Research, and the study was approved by the Animal Care and Use Committee of Yamaguchi University (approval no. 41-032). Synthetic SSSR and FGLM-NH2 peptides were synthesized by the Peptide Institute (Osaka, Japan), and the NK-1R antagonist L-733,060 was obtained from Sigma-Aldrich (St. Louis, MO, USA).
3
Exploring Nociceptive Modulation via NK1R
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The drugs used were (i) the NK1R agonist, Substance P (SP; 1 and 2 μg/μl; #S6883, Sigma, USA), (ii) the NK1R antagonist, L-733,060 (1 ×: 0.0176 µg/µl and × 0.176 µg/µl; #1145, Tocris Bioscience, UK) and (iii) the cholinergic receptor agonist, carbamoylcholine chloride (carbachol; 0.156 μg/μl; #C4382, Sigma, USA).
The functional effects of different doses of SP and L-733,060 were explored in this study. Notably, 1 × concentration of L-733,060 in the present study is anti-nociceptive on microinjection into the rostral ventral medulla48 (link). While we initially used the lower dose of L-733,060, arising from the results, especially in behaving animals, we also examined the effect of the higher dose of L-733,060 to explore whether the different aspects of nociception are differentially sensitive to antagonism by the antagonist. The concentration of carbachol used in the experiments is based on published work65 (link).
The functional effects of different doses of SP and L-733,060 were explored in this study. Notably, 1 × concentration of L-733,060 in the present study is anti-nociceptive on microinjection into the rostral ventral medulla48 (link). While we initially used the lower dose of L-733,060, arising from the results, especially in behaving animals, we also examined the effect of the higher dose of L-733,060 to explore whether the different aspects of nociception are differentially sensitive to antagonism by the antagonist. The concentration of carbachol used in the experiments is based on published work65 (link).
4
Transfected Cell Lines and Reference Agonists/Antagonists
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The transfected Chinese hamster ovary (CHO) cells, rat basophil leukemia (RBL) cells, U373 cells and BA/F3 cells were obtained from Eurofins Scientific (Le Bois I’Eveque, France). Various buffers namely Hank’s balanced salt solution (HBSS) buffer, Dulbecco’s modified Eagle medium (DMEM) buffer and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer were obtained from Invitrogen (Carlsbad, CA, USA). The reference agonists and/or antagonists atropine sulphate salt, acetylcholine chloride, [Sar9, Met(O2)11]-Substance P, [Arg8]-vasopressin (AVP), (+)butacamol, clozapine, dopamine, [d(CH2)5 1, Tyr(Me)2]-AVP, serotonin, SCH 2330, L-733,060, (S)-WAY-100635 and 3-isobutyl-1-methylxanthine (IBMX) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All the other chemicals and reagents used were purchased from E. Merck, Fluka (Rupert-Mayer-Str., Munich, Germany), and Sigma-Aldrich (St. Louis, MO, USA), unless otherwise stated, and were of highest grade available.
5
Characterization of Cell Lines for Drug Screening
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Human endogenous (U373MG cells), murine interleukin-3 dependent pro-B (Ba/F3) and a transfected Chinese hamster ovary (CHO) cell lines were obtained from Eurofins Scientific (Le Bois I’Eveque, France). Buffers—Dulbecco’s modified Eagle medium (DMEM) buffer, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer and Hank’s balanced salt solution (HBSS) buffer—were purchased from Invitrogen (Carlsbad, CA, USA). The reference agonists dopamine, [Sar9, Met(O2)11]-SP, serotonin and arginine vasopressin, and antagonists (+) butaclamol, L 733060, (S)-WAY-100635, and [d(CH2)51,Tyr(Me)2]-AVP) were obtained from Sigma-Aldrich (St. Louis, MO, USA). All other chemicals and reagents were purchased from Merck and Fluka, unless otherwise stated and were of highest available grade.
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