3 ethoxy 4 methoxyphenol

Arachidonic acid (AA: 5,8,11,14-eicosatetraenoic acid), dexamethasone [DEX: (11β, 16α)-9-fluoro-11,17,21-trihydroxy-16-methylpregna-1,4-diene-3], naproxen [NAP: (S)-(+)-2-(6-methoxy-2-naphthyl)propionic acid], esculetin (ESC: 6-hydroxy-7-methoxycoumarin), 3-ethoxy-4-methoxyphenol (EMP) were purchased from Sigma-Aldrich Korea (Seoul, Korea) and dissolved in dimethyl sulfoxide (DMSO). Leukotriene B4 (LTB4: 5S,12R-dihydroxy-6Z,8E,10E,14Z-eicosatetraenoic acid) was purchased from Cayman Chemicals (Ann Arbor, MI, USA) and dissolved in DMSO. Prostaglandin E2 (PGE2: (5Z,11α,13E,15S)-11,15-dihydroxy-9-oxoprosta-5,13-dienoic acid), prostaglandin D2 (PGD2: 9α,15S-dihydroxy-11-oxoprosta-5Z,13E-dien-1-oic acid), 2-aminoethoxydiphenylborate (2-APB), dantrolene sodium (DAN: 1-[(5-(p-nitrophenyl) furfurylidene)amino] hydantoin sodium salt), and U-73122 (1-[6-[((17β)-3-methoxyestra-1,3,5[10]-trien-17-yl)amino]hexyl]-1H-pyrrole-2,5-dione) were purchased from Sigma-Aldrich Korea and dissolved in DMSO. Fura-8AM was purchased from AAT Bioquest (Sunnyvale, CA, USA) and dissolved in DMSO. Paraquat (1,1′-dimethyl-4,4′-bipyridinium dichloride hydrate) and vitamin C (L-ascorbic acid) were purchased from Sigma-Aldrich Korea and dissolved in distilled water. Phosphate-buffered saline (PBS) was prepared with 100 mM phosphoric acid and adjusted to pH 7.4.

Identified bacterial metabolites such as Phthalimide (PM), o-Cyanobenzoic acid (CBA), and 3-Ethoxy-4-Methoxyphenol (EMP) were obtained from Sigma-Aldrich Korea (Seoul, Korea) and dimethylsulfoxide (DMSO) was used to dissolve these. Trizol reagent was collected from Invitrogen Korea (Seoul, Korea) and used for RNA extraction. Chloroform and Ethanol were collected from Sigma-Aldrich Korea (Seoul, Korea). Phosphate-buffered saline (PBS, pH 7.4) was prepared in the laboratory with 100 mM phosphoric acid and 0.7% sodium chloride. Anticoagulant buffer (ACB, pH 4.5) containing 186 mM NaCl, 17 mM Na2EDTA, and 41 mM citric acid was prepared in the laboratory.