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2 protocols using poly ethylene glycol

1

Synthesis and Characterization of Polymer-TiO2 Nanocomposites

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Poly(N-hydroxyethyl acrylamide) (pHEAAm) was synthesized by thermally initiated radical polymerization (see Supplementary Materials). Poly(acrylic acid) (pAA, Mw = 5000) and poly(vinyl pyrrolidone) (pVP, Mw = 36,000) were purchased from FUJIFILM Wako Pure Chemical Corporation (Osaka, Japan) and Honeywell Fluka (Morristown, NJ, USA), respectively, and poly(ethylene glycol) (pEG, Mw = 20,000) and poly(vinyl alcohol) (pVA, Mw = 500) were purchased from Nacalai Tesque (Kyoto, Japan). In this study, three different aqueous dispersions of titanium oxide nanoparticles (TiNP-x) were used for fabricating polymer composites. TiNP-1 (40 nm, 0.85 wt%, PTA) and TiNP-2 (20 nm, 0.85 wt%, TPX-HP) were purchased from Kon Corporation (Saga, Japan), and TiNP-3 (70 nm, 18.1 wt%, TisolA) was kindly provided by NYACOL Nano Technologies Inc. (Ashland, MA, USA). Deionized water was prepared with an Elix UV 3 (Merck KGaA, Darmstadt, Germany) and used as a solvent.
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2

Polymer-Supplemented Cell Culture Media

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High-molecular-weight (M.W.) soluble polymers and their final concentration in culture
medium (CiCM) were as follows: Poly-ethylene glycol (PEG) [M.W. 15000-25000, nacalai
tesque]: CiCM 0.5-1 mg/ml; polyvinyl alcohol (PVA) [M.W. 146000-186000, Sigma-Aldrich]:
CiCM 0.5-1 mg/ml; polyvinylpyrrolidone (PVP) [M.W. 360000, Sigma-Aldrich]: CiCM 1 mg/ml;
dextran (Dex) [M.W. 190000-230000, nacalai tesque]: CiCM 1 mg/ml; poly-γ-glutamic acid
(PGA) [M.W. 200000-500000, Wako Pure Chemical industry]: CiCM 1 mg/ml; chondroitin sulfate
C sodium salt (CSC) [M.W. 40000-80000,Wako Pure Chemical industry]: CiCM 0.5 mg/ml;
hyaluronic acid sodium salt (HRL) [M.W. not determined, Wako Pure Chemical industry]: CiCM
0.5 mg/ml; chondroitin sulfate A sodium salt (CSA) [M.W. 40000-80000, Sigma-Aldrich]: CiCM
0.5 mg/ml; dermatan sulfate sodium salt (DS) [M.W. 85000~100000, Tokyo Chemical Industry]:
CiCM 0.5 mg/ml; poly-L-lysine hydrobromide (PLL) [M.W. 30000-70000, Sigma-Aldrich] CiCM
5-50 μg/ml; poly-L-ornithine hydrobromide (PLO) [M.W. 30000-70000, Sigma-Aldrich]: CiCM
12.5 μg/ml; poly-D-lysine hydrobromide (PDL) [M.W. 30000-70000, Sigma-Aldrich]: CiCM 12.5
μg/ml; poly-L-histidine (PLH) [M.W. 5000-25000, Sigma-Aldrich]: CiCM 12.5 μg/ml.
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