Biowave pro

Manufactured by Ted Pella

Sourced in United States

The Biowave Pro is a microwave-assisted sample preparation system designed for use in laboratories. It provides controlled microwave heating to facilitate various sample preparation processes. The Biowave Pro features a compact design and user-friendly interface, allowing for efficient and consistent sample preparation.

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Lab products found in correlation

Uc7 ultramicrotome, by Leica (1 mentions) Hr25 300 mesh copper rhodium grids, by TAAB (1 mentions) Lr white resin, by Polysciences (1 mentions) Diamond knife, by Electron Microscopy Sciences (1 mentions) 1400 transmission electron microscope, by JEOL (1 mentions) Malachite green, by Merck Group (1 mentions) Uranyl acetate, by Serva Electrophoresis (1 mentions) K3fe cn 6, by Merck Group (1 mentions) Ethylene glycol tetraacetic acid (egta), by Merck Group (1 mentions) Pipes, by Merck Group (1 mentions) Durcupan, by Merck Group (1 mentions) Mgcl2, by Merck Group (1 mentions)

5 protocols using biowave pro

Dental Plaque and GCF Sample Embedding

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The dental plaque and GCF paper point samples were fixed with 4% paraformaldehyde in 0.1‐M sodium cacodylate buffer for 5 h at 4°C. Resin embedding was microwave‐assisted with a PELCO BiowavePro+ (Ted Pella). After rinsing twice with a mixture of 0.2‐M saccharose/0.1‐M sodium cacodylate and once with distilled water, samples were gradually dehydrated by successive baths in 50%, 70% and 96% ethanol. Substitution with medium grade LR–White resin (Polysciences) was achieved by two incubations with a mixture of 100% LR–White resin and 96% ethanol in a 2:1 ratio, two incubations with 100% LR–White resin, and completed with samples in 100% LR–White resin. Resin heat‐curing was achieved by polymerization for 72 h at 60°C. All solutions used above were 0.2‐μm filtered. Ultrathin 100 nm sections were cut using a UC7 ultramicrotome (Leica Microsystems) and placed on HR25 300 Mesh Copper/Rhodium grids (TAAB).

Brahim Belhaouari D., Baudoin J., Lagier J., Monnet‐Corti V., La Scola B, & Antezack A. (2022). Microscopic observations of SARS‐CoV‐2 like particles in different oral samples. European Journal of Oral Sciences, 130(6), e12903.

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Glutaraldehyde-Based Fixation and TEM Imaging

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NBS were
fixed overnight at 4 °C in a fixative
comprising 2.5% (w/v) glutaraldehyde in 0.2 M sodium phosphate buffer
(pH 7.4). NBS were not washed prior to fixation. Fixed samples were
rinsed with 0.1 M sodium phosphate buffer and post fixed in 1% osmium
tetroxide in 0.2 M sodium phosphate buffer using a BioWave Pro + microwave
tissue processor (Ted Pella, USA). After rinsing with 0.1 M sodium
phosphate buffer, samples were dehydrated in a graded series of ethanol,
infiltrated with resin (Procure, 812), and polymerized using an oven
at 60 °C for 48 h. Ultrathin sections (70 nm) were cut using
a diamond knife (Diatome) and collected onto carbon-coated copper
slot TEM grids. Grids were post-stained using uranyl acetate (2% w/v)
and lead citrate. Two grids were collected from duplicate regions
for each sample and imaged using a JEOL 1400 transmission electron
microscope (Tokyo, Japan) operating at 100 kV.

Rybchyn M.S., Biazik J.M., Charlesworth J, & le Coutre J. (2021). Nanocellulose from Nata de Coco as a Bioscaffold for Cell-Based Meat. ACS Omega, 6(49), 33923-33931.

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Spinal Cord Tissue Embedding for Microscopy

Cited 1 time

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Lumbar spinal cord sections (350μm) from immersion fixed and perfused mice were embedded using a previously described protocol (Hartley et al. 2019 (link), Calkins et al. 2019 (link)). Briefly, the spinal cord sections were post-fixed in 1.5% paraformaldehyde, 1.5% glutaraldehyde, 0.05M sucrose, 0.25% calcium chloride in 0.05M sodium cacodylate buffer pH 7.4. All embedding steps were performed using the Biowave Pro + (Ted Pella, Redding, CA). The tissue was processed using 2% osmium-1.5% potassium ferrocyanide, rinsed with distilled water, immersed in 0.5% uranyl acetate, passed through series of acetone solutions (20, 30, 50, 75, 95, 100%) for dehydration, infiltrated with acetone/resin mixtures and finally with resin (Spurr and Eponate 12). The blocks were polymerized in an oven for 1-2 days at 63°C. The blocks were removed from capsules, sectioned semi-thin (0.5 μm) for light microscopy and ultra-thin (70nm) for EM. All sections were processed without knowledge of treatment group.

Chaudhary P., Marracci G., Calkins E., Pocius E., Bensen A., Scanlan T., Emery B, & Bourdette D. (2020). Thyroid hormone and thyromimetics inhibit myelin and axonal degeneration and oligodendrocyte loss in EAE. Journal of neuroimmunology, 352, 577468.

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Microwave-assisted Cell Fixation Protocol

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For the remainder of the sample preparation procedure, all centrifugation steps were performed at ~21,000 × g for 1 min. The cell suspension in CB was centrifuged, and cells were resuspended in fresh CB and incubated for 10 min; this wash step was repeated for a total of 3 times. After the last wash step, the cells were resuspended in CB supplemented with 1% OsO₄ and 1.6% potassium ferricyanide. Using a Pelco Biowave Pro microwave tissue processor (Ted Pella; abbreviated as “MW”), the cells were fixed with 2 cycles of the following procedure: 2 min of heating in a vacuum and 2 min of vacuum without heating. To remove the OsO₄, the cells were resuspended in CB, heated for 40 s in the MW, and centrifuged, and the supernatant was removed. This wash step was repeated a total of 3 times.

Taketani M., Donia M.S., Jacobson A.N., Lambris J.D, & Fischbach M.A. (2015). A Phase-Variable Surface Layer from the Gut Symbiont Bacteroides thetaiotaomicron. mBio, 6(5), e01339-15.

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Microwave-Assisted Specimen Preparation for EM

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The entire EM processing was performed using a Ted Pella Biowave Pro microwave. After samples were lightly fixed and imaged in the confocal microscope, they were heavily fixed with 2.5% glutaraldehyde (EMS), 4% formaldehyde (EMS) and 0.05% malachite green (Sigma-Aldrich) in 0.1 M PHEM (pH 6.9: 240 mM PIPES [Sigma-Aldrich], 100 mM Hepes [Biomol], 8 mM MgCl₂ [Merck], 40 mM EGTA [Sigma-Aldrich]). The samples were then postfixed with 0.8% K₃Fe(CN)₆ (Merck), 1% OsO₄ (Serva) in 0.1 M PHEM. The samples were stained successively with 1% tannic acid (EMS) and 1% uranyl acetate (Serva) to enhance the contrast. Samples were dehydrated in a graded ethanol series (30, 50, 75, 90%, 2 × 100%) and infiltrated in a graded series of Durcupan (25, 50, 75, 90%, 2 × 100%, Sigma-Aldrich) and polymerized in the oven at 60°C for 96 h.

Serra Lleti J.M., Steyer A.M., Schieber N.L., Neumann B., Tischer C., Hilsenstein V., Holtstrom M., Unrau D., Kirmse R., Lucocq J.M., Pepperkok R, & Schwab Y. (2022). CLEMSite, a software for automated phenotypic screens using light microscopy and FIB-SEM. The Journal of Cell Biology, 222(3), e202209127.

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