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Polyvinyl chloride

Manufactured by Merck Group
Sourced in United States, Germany, France

Polyvinyl chloride (PVC) is a synthetic polymer material used in the production of various laboratory equipment. It is a thermoplastic resin known for its durability, chemical resistance, and versatility. PVC is commonly used in the manufacture of tubing, containers, and other lab-related components due to its functional properties.

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35 protocols using polyvinyl chloride

1

Synthesis of Marked Particles for Enzymatic Digestion

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For the synthesis of marked particles, polyvinyl chloride (Mw 43 kDa, PVC), tetrahydrofuran (THF), sodium chloride (NaCl), and platinum octaethylporphyrin (PtOEP) were purchased from Merck (Milan, Italy).
For the enzymatic digestion, α-Amylase from Bacillus sp. (A4862-250ML, liquid form), Lipase from Aspergillus niger (62301, powder), Papain from Carica papaya (10108014001 Roche, Basel, Switzerland, stock concentration 100 mg/10 mL, suspension form) and sodium dodecyl sulphate (SDS, liquid form) were supplied by Sigma-Aldrich (Darmstadt, Germany) such as sucrose and zinc chloride (ZnCl2) used for the gradient density separation.
For the filtration step, the membrane filters were made by Whatman®, Sigma-Aldrich (Darmstadt, Germany) (filters of cellulose nitrate 12 μm and aluminum oxide (Anodisc) 0.1 μm were used).
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2

Ion-Selective Electrode Fabrication

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The chemicals used in this study were of analytical grade. 1-(Pyridin-4-yl)ethan-1-one, 1-bromododecane, 4-amino-1,5-dimethyl-2-phenyl-1,2-dihydro-3H-pyrazol-3-one, o-nitrophenyl octyl ether (o-NPOE), dioctyl sebacate (DOS), chloronaphthalene (CN), dioctyl adipate (DOA), dioctyl phthalate (DOP), potassium tetrakis(4-chlorophenyl) borate (KTpClPB), and dibutyl phthalate (DBP) were obtained from Sigma-Aldrich. Tetrahydrofuran (THF), ethanol, and polyvinyl chloride (PVC) were purchased from Merck. Deionized water was applied throughout to prepare all aqueous solutions. Nitrate salts of cations were used for selectivity studies.
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3

PVC as Carbon Source Protocol

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Instead of carbon source, polyvinyl chloride (sigma-Aldrich) was used as a carbon source (3 g/100 mL) on agar plate for 3 months and with one-year time incubation in broth.
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4

Electrochemical Sensor Development Protocol

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Polyvinyl chloride (PVC, selectophore, high molecular weight), agarose (low EEO), potassium tetrakis(4-chlorophenyl)borate (selectophore, ≥ 98%), sodium ionophore X (selectophore, function tested), α-d-glucose (anhydrous, 96%), pyruvic acid (98%), and AuNPs(20 nm diameter, stabilized suspension in citrate buffer) were supplied by Sigma-Aldrich Co., LLC (Japan). Phosphate-buffered saline (PBS(-), pH 7.1), 0.05 M Tris–HCl buffer solution (pH 7.6), tetrahydrofuran (THF, 99.5%, with stabilizer), potassium chloride (KCl, 99.5%), sodium chloride (NaCl, 99.5%) and ammonium chloride (NH4Cl, 99.5%), bis(2-Ethylhexyl) sebacate (DOS), magnesium chloride (MnCl2, 97%), urea (99%), and UA (98%) were purchased from FUJIFILM Waco Pure Chemical Corporation (Japan). Carbon grease (CG, particle size < 40 nm) was provided by KITACO Co., Ltd (Japan). Ag/AgCl ink for reference electrode was obtained from ALS Co., Ltd. PC plates were supplied by HAKUDO Corporation. CPE (MS-550) was purchased from Ecologa Japan Co., Ltd.
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5

Electro-Analytical Sensor Fabrication

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Tetracycline (TC), N-hydroxysuccinimide (NHS), 11-amino-1-undecanethiol, phosphate buffer solution (PBS) tablets, 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC), Ethanol, sodium dodecyl sulfate (SDS), Polyvinyl chloride (PVC), o-nitro phenyl octyl ether (NPOE), di-octyl phthalate (DOP), dibutyl phthalate (DBP), dibutyl sebacate (DBS), hydrochloric acid (HCl), diethyl ether, tetrahydrofuran (THF), di-octyl phthalate, bis(2-ethyl hexyl) sebacate were purchased from Sigma-Aldrich (France). The standard solutions and buffers were prepared with Millipore Milli-Q nanopure water (resistivity > 18 MW cm) which is produced by a Millipore Reagent Water System (France). Epoxy resin EPO TEK H70E 2LC was from Epoxy Technology, France. Cs[o-COSAN], was synthesized from 1,2-closo-C2B10H12 from Katchem Spol.sr.o (Kralupy nad Vltavou, Czech Republic), as reported in the literature [54 (link)]. The Na[o-COSAN] was obtained by means of cationic exchange resin from Cs[o-COSAN] following the previously described procedure [31 (link)].
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6

Fabrication of Enzymatic Biosensor Prototype

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Chitosan, acetic acid, polyvinyl chloride, tetrahydrofuran, bovine serum albumin, L-lactic acid, sodium phosphate monobasic and sodium phosphate dibasic were obtained from Sigma-Aldrich (St Louis, MO). L-LOx (activity, 101 U mg−1) was procured from Toyobo Corp. (Osaka, Japan). All reagents were used without further purification. Prussian blue conductive carbon (C2070424P2), Ag/AgCl (E2414) and insulator (Dupont 5036) inks were procured from Gwent Group (Pontypool, UK), Ercon Inc. (Wareham, MA) and Dupont (Wilmington, DE). Electrocardiogram hydrogel conductive adhesive (RG63B, 35-mil thick) was purchased from Covidien. Polyester sheets (MELINEX 453, 50-μm thick) were provided by Tekra Inc. (New Berlin, WI).
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7

Wavelength Conversion Luminescent Resin Compositions

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Example 3

1 g of polymethyl methacrylate (manufactured by Sigma-Aldrich Co. LLC.) was dissolved in 10 mL of toluene, then 10 mg of Compound (3) was added to prepare a coloring agent resin solution which was then spin-coated on a glass plate at 2000 rpm and dried on a hot plate at 50° C. to prepare a wavelength conversion luminescent resin composition (wavelength conversion member) (see paragraph [0081] of JP2011-241160A).

Example 5

A wavelength conversion luminescent resin composition (wavelength conversion member) was prepared in the same manner as in Example 3, except that polymethyl methacrylate was replaced by polyvinyl chloride (manufactured by Sigma-Aldrich Co. LLC.).

Example 7

A wavelength conversion luminescent resin composition (wavelength conversion member) was prepared in the same manner as in Example 3, except that polymethyl methacrylate was replaced by polycarbonate (manufactured by Kanto Chemical Co., Ltd.).

Example 8

A wavelength conversion luminescent resin composition (wavelength conversion member) was prepared in the same manner as in Example 3, except that polymethyl methacrylate was replaced by triacetyl cellulose (manufactured by Wako Pure Chemical Industries, Ltd.), and toluene was replaced by methylene chloride.

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8

Wavelength Conversion Luminescent Resin Compositions

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Example 3

1 g of polymethyl methacrylate (manufactured by Sigma-Aldrich Co. LLC.) was dissolved in 10 mL of toluene, then 10 mg of Compound (3) was added to prepare a coloring agent resin solution which was then spin-coated on a glass plate at 2000 rpm and dried on a hot plate at 50° C. to prepare a wavelength conversion luminescent resin composition (wavelength conversion member) (see paragraph [0081] of JP2011-241160A).

Example 5

A wavelength conversion luminescent resin composition (wavelength conversion member) was prepared in the same manner as in Example 3, except that polymethyl methacrylate was replaced by polyvinyl chloride (manufactured by Sigma-Aldrich Co. LLC.).

Example 7

A wavelength conversion luminescent resin composition (wavelength conversion member) was prepared in the same manner as in Example 3, except that polymethyl methacrylate was replaced by polycarbonate (manufactured by Kanto Chemical Co., Ltd.).

Example 8

A wavelength conversion luminescent resin composition (wavelength conversion member) was prepared in the same manner as in Example 3, except that polymethyl methacrylate was replaced by triacetyl cellulose (manufactured by Wako Pure Chemical Industries, Ltd.), and toluene was replaced by methylene chloride.

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9

Electrochemical Sensor Fabrication and Materials

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Graphite flake and silver nitrate were purchased from Alfa Aesar. Sodium nitrate, potassium permanganate, hydrogen peroxide, multiwalled CNTs, TTF, citric acid, chloroplatinic acid, sodium borohydride, ammonium chloride, nickel chloride, copper acetate, iron(III) chloride, copper acetate, zinc nitrate, Nafion perfluorinated resin solution (5%), sodium thiosulfate pentahydrate, sodium bisulfite, polyvinyl chloride (PVC), polyvinyl butyral (PVB), F127, nonactin, bis(2-ethylhexyl) sebacate (DOS), 3,4-ethylenedioxythiophene (EDOT), poly(sodium 4-styrenesulfonate) (PSS), CS, PDMS, aniline, and platinum carbon black (Pt/C) (0.5%) were purchased from Sigma-Aldrich. Sulfuric acid, hydrochloric acid, ascorbic acid, methanol, ethanol, acetone, tetrahydrofuran, sodium chloride, disodium phosphate, urea, and dextrose (D-glucose) were purchased from Fisher Scientific. MDB was purchased from Combi- Blocks Inc. Lactate oxidase (106 U mg−1) was purchase from Toyobo Co. The CNT film and Ni substrate (h-Ni) were purchased from NTL Inc. and MXBaoheng Products, respectively. Medical tapes were purchased from Adhesives Research. PIs (PI-2611 and PI-2610) were purchased from HD MicroSystems Inc. Silver conductive paint was purchased from Structure Probe, Inc. (SPI supplies).
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10

Fluorescent Labeling of Thiol-Containing Molecules

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Polyvinyl chloride (average Mw = 233,000, Mn = 99,000) (PVC), ethylenediamine, 5,5’-dithiobis (2-nitrobenzoic acid), triethylamine, 1,4,8,11-tetraazacyclotetradecane (cyclam), N-acetyl-d-penicillamine (Fluka), and concentrated hydrochloric acid were obtained from Sigma-Aldrich (St. Louis, MO, USA). ATTO-TAGTM FQ Amine-Derivatization Kit was purchased from Invitrogen (Grand Island, NY, USA). Tert-butyl nitrite (90% technical grade, Acros Organics) was purchased from Fisher Scientific. Magnesium sulfate (MgSO4), copper (II) bromide (CuBr2), L-ascorbic acid sodium salt, and acetic anhydride (Alfa Aesar) were purchased from VWR (West Chester, PA, USA).
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