5 × 105−ΔΔCT CT26 cells were subcutaneously injected to the right flank of mice to establish xenograft colon tumor model. When the tumor volume reached about 100 mm3 (day 7), 50 μL of saline, free ATO solution (50 mg/kg), free CTX solution (25 mg/kg), free ATO + CTX solution (50 mg/kg ATO +25 mg/kg CTX), or hydrogel containing ATO (ATO@Gel, 50 mg/kg), CTX (CTX@Gel, 25 mg/kg), or their combination (ATO + CTX@Gel, 50 mg/kg ATO +25 mg/kg CTX) was injected into the tumor using BD Ultra-Fine insulin syringe (29 G), respectively. Tumor surface with intact skin were chosen as the injection site. The tumor volume was computed according to the formula: (length×width2)/2. At the end point of experiment, mice were sacrificed. Subcutaneous tumors were separated for further use. The blood, heart, liver, spleen, lung and kidney were collected for safety evaluation.
Ultra fine insulin syringe
Manufactured by BD
Sourced in United States
BD Ultra-Fine Insulin Syringes are designed for the administration of insulin. The syringes feature a thin, short needle and are intended for subcutaneous injection. The syringes are available in different sizes to accommodate various insulin requirements.
Automatically generated - may contain errors
Lab products found in correlation
Male balb c mice, by Nara Biotech (1 mentions)
Dm il led, by Leica (1 mentions)
31 g needle, by BD (1 mentions)
Ivis 200 imaging system, by PerkinElmer (1 mentions)
Balb c nude mice, by Orient Bio (1 mentions)
Hamilton syringe, by Hamilton Company (1 mentions)
Sodium borohydride, by J&K Scientific (1 mentions)
Cetyltrimethylammonium chloride, by J&K Scientific (1 mentions)
Chloroauric chloride, by Sinopharm (1 mentions)
Dmem high glucose medium, by Thermo Fisher Scientific (1 mentions)
Trypsin edta, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (1 mentions)
Methanol, by Sinopharm (1 mentions)
Ascorbic acid, by Sinopharm (1 mentions)
Sodium hydroxide (naoh), by Sinopharm (1 mentions)
Tetraethyl orthosilicate, by Sinopharm (1 mentions)
Ad ctrl, by Hanbio Biotechnology (1 mentions)
Petri dish, by SPL Life Sciences (1 mentions)
22 protocols using ultra fine insulin syringe
1
Subcutaneous Xenograft Colon Tumor Model and Drug Delivery
2
Subcutaneous Biocompatibility Evaluation of W-Paste
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Male BALB/c mice (20–30 g) were purchased from Nara Biotech Co., Ltd. (Seoul, Korea). Avertin (anesthetic; 2.5%), IVD Black silk (11 mm, AILEE), BD Ultra-Fine Insulin Syringe [1 mL, 0.25 mm (31 G) × 8 mm], blood collection tubes [with K2E ethylenediaminetetraacetic acid (EDTA) 18.0 mg, 10.0 mL], and 4% paraformaldehyde solution (BYLABS) were used.
The biocompatibility of the W-paste was assessed in 15 BALB/c mice (male, 6 weeks old). The mice were divided into PLGA, PDMS, and W-paste groups as positive control, negative control, and test sample, respectively after a diurnal cycle for 1 week before the surgery. The mice wereanesthetized using an intraperitoneal injection of 2.5% avertin.
Each sample was prepared with a size of 5 mm × 5 mm thickness of 100 μm. Prior to implantation, the test and control materials were sterilized using UV irradiation. The sterilized materials were then implanted in subcutaneous mouse tissues on the dorsal side of the animal for four weeks. The body weights of the mice in the test and control groups were measured weekly for four weeks after implantation.
The biocompatibility of the W-paste was assessed in 15 BALB/c mice (male, 6 weeks old). The mice were divided into PLGA, PDMS, and W-paste groups as positive control, negative control, and test sample, respectively after a diurnal cycle for 1 week before the surgery. The mice wereanesthetized using an intraperitoneal injection of 2.5% avertin.
Each sample was prepared with a size of 5 mm × 5 mm thickness of 100 μm. Prior to implantation, the test and control materials were sterilized using UV irradiation. The sterilized materials were then implanted in subcutaneous mouse tissues on the dorsal side of the animal for four weeks. The body weights of the mice in the test and control groups were measured weekly for four weeks after implantation.
3
Plasmid DNA Delivery via Pluronic L64 and Electrotransfer
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Pluronic L64 (L64; Sigma-Aldrich, St Louis, MO, USA) solution was diluted to 0.2% (w/v) using saline, mixed with equal volume of plasmid DNA (pDNA) and kept for 5 min at room temperature before injection. Each side of the TA muscle in a mouse was injected with 30 μL saline containing 30 μg pDNA/L64 (0.1%) mixture using a 29-gauge BD Ultra-Fine insulin syringe (BD, Franklin Lakes, NJ, USA). The injected site was approximately in the middle of a TA muscle and the injection time was 2–3 s. An hour after the injection, a clinically applied SDZ-V Nerve and Muscle Stimulator was used for electrotransfering. The electropulse parameters were: 5 Hz, intensity level of 3 and stimulation duration as 3 min. In order to increase the therapeutic effect of mLFVII-Fc, each mouse was injected with a therapeutic or control plasmid once a week.
4
In Vitro and In Vivo Evaluation of Macrophage Response
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Mouse macrophages were cultured in 24-well plates at 5 × 104 cells per well for 24 h for the proinflammatory cytokine enzyme-linked immunosorbent assay (ELISA). The incubated cells were treated with eluates. Seven days after the treatment, the supernatant was collected and centrifuged to remove any cell fragments. After the centrifugation, the TNFα, IL-1β, and IL-6 protein expressions were detected using ELISA kits.
Four weeks after the implantation, mouse blood samples were collected using cardiac puncture with a BD ultrafine insulin syringe to determine the blood chemistry. Then, the mice were sacrificed by cervical dislocation, and the skin tissue surrounding the implanted materials was carefully harvested and immediately fixed using 4% paraformaldehyde. The collected tissues were blocked using paraffin embedding, sliced using a microtome, and stained with Masson's trichrome and hematoxylin and eosin (H&E) reagents. The structure of the skin tissues was analyzed using an optical microscope (OM; DM IL LED, Leica, Germany).
Four weeks after the implantation, mouse blood samples were collected using cardiac puncture with a BD ultrafine insulin syringe to determine the blood chemistry. Then, the mice were sacrificed by cervical dislocation, and the skin tissue surrounding the implanted materials was carefully harvested and immediately fixed using 4% paraformaldehyde. The collected tissues were blocked using paraffin embedding, sliced using a microtome, and stained with Masson's trichrome and hematoxylin and eosin (H&E) reagents. The structure of the skin tissues was analyzed using an optical microscope (OM; DM IL LED, Leica, Germany).
5
AAV-Mediated Gene Delivery in Mice
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AAV was administered to 8-week-old young adult male C57BL/6J mice anaesthetized with 2–4% isoflurane. The mice were injected intramuscularly with AAV9-CjCas9 (1 × 1011 viral genome) in physiological saline (40 μl) using an ultra-fine insulin syringe with a 31 G needle (BD). As a negative control, C57BL/6J mice were injected with physiological saline (40 μl) only.
6
Microsurgery and Imaging of Plant Shoot Apices
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Microsurgery was performed by making an incision between the SAM and incipient primordium (I1) using a syringe needle (0.3 mm, BD Ultra-Fine Insulin Syringe) under a dissecting microscope as previously described [47 (link)]. The incised shoot apices were allowed to grow for four days, followed by transverse agarose gel sectioning of radialized leaf primordial generated from the incised I1.
Tissue fixation for scanning electron microscopy was carried out using a quick method as previously described. Briefly, shoot apices were fixed in pure methanol for 15 min and then dehydrated in 100% ethanol for 30 min at room temperature. After one change of 100% ethanol, the tissues were stored in 100% ethanol overnight at room temperature. Tissues were dried with CO2 in a critical point drier and coated with gold in a sputter coater. Tissues were imaged using a Hitachi S-3000N variable pressure scanning electron microscope at an accelerating voltage of 5 kV. For scanning flower organs, fresh plant materials were observed with HIROX SH-3000 tabletop microscope equipped with a cool stage. The temperature was set at −20°C and accelerating voltage was 5 kV.
Tissue fixation for scanning electron microscopy was carried out using a quick method as previously described. Briefly, shoot apices were fixed in pure methanol for 15 min and then dehydrated in 100% ethanol for 30 min at room temperature. After one change of 100% ethanol, the tissues were stored in 100% ethanol overnight at room temperature. Tissues were dried with CO2 in a critical point drier and coated with gold in a sputter coater. Tissues were imaged using a Hitachi S-3000N variable pressure scanning electron microscope at an accelerating voltage of 5 kV. For scanning flower organs, fresh plant materials were observed with HIROX SH-3000 tabletop microscope equipped with a cool stage. The temperature was set at −20°C and accelerating voltage was 5 kV.
7
Notexin-Induced Skeletal Muscle Injury
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Single injury was performed by carefully shaving the anterior hindlimb before intramuscular injection of 40 µl notexin (5 μg/ml, Latoxan, #L8104) into the tibialis anterior using a 0.3 ml ultrafine insulin syringe (BD Biosciences, #324906). Immediately prior to injection, the needle was dipped in green tattoo dye (Harvard Apparatus, #72-9384) to mark the needle track. The contralateral leg was left uninjured as a control. Mice were allowed to recover for 28 days before the animal was euthanized and muscles collected for analysis. For repeat injuries we performed 3 separate intramuscular notexin injections, each 14 days apart, and allowed 28 days of recovery following the final injury before tissue collection. Using the superficial mark on the skin from the tattoo dye, we attempted to perform each injury as close to the site of the previous injury as possible so as to repeatedly injure the same myofibers each time. For AnxA2-notexin studies, 10 µg recombinant AnxA2 (RayBiotech, #230-30023) was added to 40 µl notexin (5 μg/ml, Latoxan, #L8104) and injected into the mid-belly of the right tibialis anterior. The contralateral (left) tibialis anterior was injected with 40 µl notexin only for comparison. Again, these muscles were harvested for analysis 28 days after injury.
8
Tracking Stem Cells via Nanoparticles
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hMSCs (3 × 105 cells/well) were seeded in 6-well plates, after which they were rinsed twice, and pDNA-coupled SF-NPs were added. After 6 h, the cells were detached to obtain SF-NP-treated T1 cells, and the remaining T1 cells were subcultured three times to obtain T4 cells. To evaluate cellular tracking of hMSCs transfected with pDNA-coupled SF-NPs, T1, T2, T3, and T4 cells (3 × 106 cells) were xenotransplanted into 7-week-old male BALB/c nude mice (Orient-Bio, Seongnam, Korea). Specifically, transfected hMSCs were suspended in 50 µl of DPBS and subcutaneously injected into the flank using a 29-gauge Ultra-Fine™ insulin syringe (#320320, Becton-Dickinson, NE, USA). The animal study was approved by the Institutional Animal Care and Use Committee (IACUC) of CHA. For in vivo optical imaging, the transplanted mice were imaged with an IVIS Imaging System 200 (Perkin Elmer, Santa Clara, CA, USA).
9
Kanamycin Administration via Diverse Methods
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Kanamycin (KM) sulphate was diluted in normal saline (150 mg/ml) and administered in three different ways (Figure 1 ). For the KP group, 50 ul of the drug solution was delivered by injection with an insulin syringe (Ultrafine Insulin Syringe, Becton Dickinson, USA) to the bulla. To improve the absorption to the RWM, the animal was laid on its contralateral side, injected, and sustained for half an hour. For both KG and KI groups, the bulla was exposed through a retroauricular skin incision. After anesthetization, furs near the bulla were removed and the skin was also incised. A small hole was made on the bulla and the RWM was exposed. A small gelfoam was placed on the RWM and 4 microliters of kanamycin solution was injected with a Hamilton syringe (Hamilton Company, Nevada, USA) for the KG group. In the case of the KI group, a small hole was made on the bulla and the tip of a cannula connected with the Hamilton syringe was fitted into the RWM after the endolymph liquid was drained. Then, 4 microliters of the KM solution was gently and slowly injected. Animals in both groups were also laid on their contralateral side for surgery for stable absorption of the drug.
10
Anesthesia-Guided Animal Allergy and Cell Transplant Procedures
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The IT OVA administration for the allergen challenge, MSC injection, and blood
and bronchoalveolar lavage fluid (BALF) collection were performed under
anesthesia with 100 mg/kg 5% ketamine hydrochloride (König, Santana de Parnaíba,
SP, Brazil) and 10 mg/kg 2% xylazine hydrochloride (Vetbrands, Paulínia, SP,
Brazil). To avoid a reduction in body temperature during anesthesia, the animals
were placed in a supine position on a heated table (Master Digital SA-300;
Ch@mpion Eletronics, Porto Alegre, RS, Brazil). A small incision (approximately
1 cm) in the skin was performed over the medial ventral cervical region (over
the trachea) in the craniocaudal direction. The ventral cervical muscles were
divulsed, and after the identification of the trachea, the solutions (Allergenic
challenge: saline or OVA; Transplantation: saline or MSCs) were administered by
a 0.3-ml ultrafine insulin syringe (Becton Dickinson, Curitiba, PR, Brazil).
and bronchoalveolar lavage fluid (BALF) collection were performed under
anesthesia with 100 mg/kg 5% ketamine hydrochloride (König, Santana de Parnaíba,
SP, Brazil) and 10 mg/kg 2% xylazine hydrochloride (Vetbrands, Paulínia, SP,
Brazil). To avoid a reduction in body temperature during anesthesia, the animals
were placed in a supine position on a heated table (Master Digital SA-300;
Ch@mpion Eletronics, Porto Alegre, RS, Brazil). A small incision (approximately
1 cm) in the skin was performed over the medial ventral cervical region (over
the trachea) in the craniocaudal direction. The ventral cervical muscles were
divulsed, and after the identification of the trachea, the solutions (Allergenic
challenge: saline or OVA; Transplantation: saline or MSCs) were administered by
a 0.3-ml ultrafine insulin syringe (Becton Dickinson, Curitiba, PR, Brazil).
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