Na2hpo4

Methylglyoxal (40% in water), glyoxal (40% in water), 2,2-diphenyl-1-picrylhydrazyl,2,2-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid), methanol (HPLC grade), acetonitrile (HPLC gradient grade and LC-MS grade), water (LC-MS grade), diosmin, diosmetin, hesperidin, hesperetin, Trolox, metformin hydrochloride, bovine serum albumin, DMSO, 98–100% formic acid, 2-methylquinoxaline, quinoxaline, o-phenylenediamine, 2, 4, 6-tripyridyl-s-triazine, iron(III) chloride hexahydrate, and iron(II) sulfate heptahydrate were purchased from Merck-Sigma-Aldrich (Sigma-Aldrich Sp. z o.o., Poznań, Poland); NaCl, KCl, Na₂HPO₄, and KH₂PO₄ (reagent grade) were obtained from Chempur (Piekary Śląskie, Poland); quercetin and rutin were from Extrasynthese (Genay Cedex, France); calcium dobesilate was purchased from PPF Hasco-Lek S. A. (Wroclaw, Poland). Water used in the study was glass-distilled and deionized. The stock solutions of standards (3 mM) were prepared by dissolving the appropriate amount of a reference compound in 5 mL of methanol or DMSO. Working standard solutions for the derivatization experiment in the range of 5–210 μM were made by mixing with 50% aq. (aqueous) methanol (v/v), filtered through hydrophilic Millex Syringe Filters (Durapore 0.22 μm; Millipore, Burlington, MA, USA) and stored at −20 °C.

l-Cysteine (Cys), gold nanoparticles (diameter 20 nm, stabilized suspension in citrate buffer), protein crosslinker 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), amine-reactive crosslinker N-hydroxysuccinimide ester (NHS), anti-norovirus (MNV-1) mouse monoclonal antibody, clone 5C4.10, Cat. No. MABF2097, 0.10 mg mL⁻¹ (Ab), bovine serum albumin (BSA), norovirus, ferrocyanide, and glutaraldehyde (GA) were purchased from Sigma-Aldrich Co (Merck company). Sulfuric acid (H₂SO₄), citric acid (CA), NaOH, NaH₂PO₄, Na₂HPO_4, KH₂PO₄, HCl, and CH₃COOH were purchased from Chempur. NaCl, KCl, and CH₃COONa were purchased from POCH (Part of Avantor, Performance Materials, Poland). Tris was purchased from Roche. All chemicals were of analytical grade and were used without any further purification. All buffers were prepared according to generally known, obligatory standards. Fruits, vegetables, and oysters for analysis in the food samples were obtained from the Selgros store. Bacteria: E. coli, B. subtilis, Salmonella spp, E. faecalis, were obtained courtesy of Prof. Jaroslaw Król from the Department of Pathology, Wrocław University of Environmental and Life Sciences, Poland.

Glycidol and 1,1,1-tris(hydroxymethyl)propane
were purchased from Sigma-Aldrich. 2,2-Dimethoxypropane, benzoyl chloride,
and phenyl isocyanate were purchased from Alfa Aesar. Anhydrous pyridine
was purchased from Acros Organics. PTSA (Sigma-Aldrich) was dried
with benzene. Glycidol was dried with 4 Å molecular sieves and
distilled under reduced pressure. Comonomer 2-acrylamidephenylboronic
acid pinacol ester, 2-AAPBAE, was synthesized according to the procedure
reported in ref (33 (link)). The α,α′-azobis(isobutyronitrile), AIBN (Fluka),
was recrystallized from methanol. Clotrimazole (Sigma-Aldrich) was
used as received. Dialysis tubes (SnakeSkin TM 3.5 K MWCO) were purchased
from Thermo Fisher Scientific. Surfactant-free cellulose acetate (SFCA,
0.8 μm) filters were purchased from Sartorius. Deionized water
was prepared in SolPure XIO P (Elkar, Poland), where conductivity
was equal to 0.055 μS. Na₂HPO₄ and KH₂PO₄ were purchased from Chempur. Tween80 was purchased
from Karl Roth. Acetonitrile for HPLC-super gradient was purchased
from POCH.