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Anti total h3

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Anti-total H3 is a laboratory reagent that can be used to detect and quantify total histone H3 protein levels in cell and tissue samples. It is a specific antibody that binds to the histone H3 protein, allowing for its identification and measurement using various analytical techniques.

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Lab products found in correlation

Cell tak, by BD (2 mentions) Anti pcna, by Santa Cruz Biotechnology (2 mentions) Anti tubulin, by Merck Group (2 mentions) Anti h3k4me3, by Merck Group (2 mentions) Anti h3k4me1, by Merck Group (2 mentions) Anti rad51, by Santa Cruz Biotechnology (2 mentions)

Spelling variants of this product

Anti-H3 (36 citations) Total H3 (5 citations)

2 protocols using anti total h3

1

Immunofluorescence of Key Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies were used at the following dilutions: anti-tubulin (1:15,000, Sigma), anti-H3K4me3 (1:5000, Millipore), anti-H3K4me1 (1:5000, Millipore), anti-RAD51 (1:50, Santa Cruz), anti-PCNA (1:2000, Santa Cruz), anti-total H3 (1:1000 Millipore), and anti-Mre11 (1:10,000, gift from J. Petrini, MSKCC). MEFs were prepared for immunofluorescence by growth on 18 mm × 18 mm glass cover slips. Lymphocytes were dropped onto slides coated with CellTak (BD Biosciences). Cells were fixed with methanol and incubated with primary antibody as indicated.
Chaudhuri A.R., Callen E., Ding X., Gogola E., Duarte A.A., Lee J.E., Wong N., Lafarga V., Calvo J.A., Panzarino N.J., John S., Day A., Crespo A.V., Shen B., Starnes L.M., de Ruiter J.R., Daniel J.A., Konstantinopoulos P.A., Cortez D., Cantor S.B., Fernandez-Capetillo O., Ge K., Jonkers J., Rottenberg S., Sharan S.K, & Nussenzweig A. (2016). Replication Fork Stability Confers Chemoresistance in BRCA-deficient Cells. Nature, 535(7612), 382-387.
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2

Immunofluorescence of Key Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols
Primary antibodies were used at the following dilutions: anti-tubulin (1:15,000, Sigma), anti-H3K4me3 (1:5000, Millipore), anti-H3K4me1 (1:5000, Millipore), anti-RAD51 (1:50, Santa Cruz), anti-PCNA (1:2000, Santa Cruz), anti-total H3 (1:1000 Millipore), and anti-Mre11 (1:10,000, gift from J. Petrini, MSKCC). MEFs were prepared for immunofluorescence by growth on 18 mm × 18 mm glass cover slips. Lymphocytes were dropped onto slides coated with CellTak (BD Biosciences). Cells were fixed with methanol and incubated with primary antibody as indicated.
Chaudhuri A.R., Callen E., Ding X., Gogola E., Duarte A.A., Lee J.E., Wong N., Lafarga V., Calvo J.A., Panzarino N.J., John S., Day A., Crespo A.V., Shen B., Starnes L.M., de Ruiter J.R., Daniel J.A., Konstantinopoulos P.A., Cortez D., Cantor S.B., Fernandez-Capetillo O., Ge K., Jonkers J., Rottenberg S., Sharan S.K, & Nussenzweig A. (2016). Replication Fork Stability Confers Chemoresistance in BRCA-deficient Cells. Nature, 535(7612), 382-387.
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