Mini 1240 uv spectrophotometer

Manufactured by Shimadzu

Sourced in Japan

The Shimadzu Mini 1240 UV spectrophotometer is a compact, single-beam instrument designed for UV-visible spectroscopy. It offers a wavelength range of 190 to 1100 nm and can be used to measure the absorbance, transmittance, or concentration of various samples.

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Lab products found in correlation

Evolution 220, by Thermo Fisher Scientific (1 mentions) Dv215cd, by Ohaus (1 mentions) Autoanalyzer, by Beckman Coulter (1 mentions) Sandwich elisa kit, by BioAssay Systems (1 mentions) 5 bromo 4 chloro 3 indolyl β d galactopyranoside x gal, by Merck Group (1 mentions) Malvidin 3 o glucoside, by Merck Group (1 mentions) Ph meter, by Crison (1 mentions)

Close spelling variants of this product

UV-mini-1240 UV spectrophotometer UV-Vis Spectrophotometer UV/mini 1240 Mini 1240 UV-Vis spectrophotometer Mini UV 1240 Mini 1240 spectrophotometer UV 1240 spectrophotometer UV-Mini spectrophotometer Mini 1240 UV-1240 UV spectrophotometer

12 protocols using mini 1240 uv spectrophotometer

Comprehensive Beer Characterization Protocol

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The apparent extract of the wort and beer was determined using a bench densitometer (Rudolph Research Analytical, Tecnal), and the results are expressed in °Plato. The pH was determined using a digital pH meter (PH-1700; Instrutherm). The bitterness was analyzed using a spectrophotometer (Evolution 220, Thermo Scientific) at a wavelength of 275 nm. The color of the beer was also analyzed using a spectrophotometer (Spectrophotometer UV mini 1240, Shimadzu) at a wavelength of 430 nm, with water as the blank [23 ]. The analyses were performed in triplicate, and all of the results are shown as means ± standard deviation.
During fermentation, samples were removed at regular intervals, placed into Eppendorf tubes and analyzed as described below. Prior to the analysis, the beer samples were degassed by vigorous agitation of the placing Eppendorf tubes for one minute followed by centrifuged at 4000 rpm for 10 minutes. The supernatant was used to follow and quantify fermentation compounds: alcohols (ethanol, glycerol and methanol), carbohydrates (glucose, fructose, maltose and maltotriose), organic acids (citric acid, ascetic acid, lactic acid, succinic acid and formic acid) and esters (ethyl acetate, isoamyl acetate, isobutyl acetate, phenyl ethyl acetate, ethyl caproate and ethyl caprylate).

Nunes C.D., de Carvalho G.B., da Silva M.L., da Silva G.P., Machado B.A, & Uetanabaro A.P. (2017). Cocoa pulp in beer production: Applicability and fermentative process performance. PLoS ONE, 12(4), e0175677.

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Genomic DNA Extraction with PVP

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Total genomic DNA was extracted as described by Khadari et al. (2004) Courtaboeuf, France) optimized by adding 1% polyvinylpyrrolidone (PVP 40.000) to the buffer AP1. DNA was quantified using spectrometric method (Sambrook et al., 1989) : the absorbance was recorded at 260 nm wavelength (Spectrophotometer UV mini-1240, Shimadzu Japan). The relative purity of extracted DNA was estimated after electrophoresis with 0.7% agarose gel stained by 0.2 µg.l -1 Ethidium Bromide and immersed in 90 mM Tris-Borate, pH 8 and 10 mM Ethyldiamine Tetra-acetic Acid (EDTA), the DNA samples were visualized under ultraviolet light.

AMEDJKOUH H., & Bouguedoura N. (2021). ASSESSMENT OF THE GENETIC DIVERSITY BETWEEN TWO VARIETIES OF WONDERBOOM (FICUS SALICIFOLIA VAHL.) BY USING SIMPLE SEQUENCE REPEAT (SSR) MARKERS. Applied Ecology and Environmental Research, 19(5), 3823-3835.

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Spectrophotometric Biomass Quantification

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The equipment used was a Spectrophotometer UV mini-1240 (Shimadzu™), quartz cuvettes with 1 cm optical path and capacity of 700 µL, analytical balance model DV215CD (Ohaus™), water purification system (Millipore™), and ultrasound equipment Ultrasonic Cleaner (Unique™).

Nascimento P.A.d., Kogawa A.C., & Salgado H.R.N. (2021). A new and ecological miniaturized method by spectrophotometry for quantification of vancomycin in dosage form. Drug Analytical Research, 5(1), 39-45.

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Biochemical Markers in Heart Samples

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The blood samples were collected immediately from the heart and centrifuged at 3000× g for 15 min. The serum was stored at –40 °C for analysis of biochemical parameters. Serum MDA levels were determined by the method described by Buege and Aust [19]. Briefly, MDA was reacted with thiobarbituric acid by incubating for 15 min at 100 °C. MDA levels were measured spectrophotometrically at 535 nm using the 1240 UV mini spectrophotometer (SHIMADZU). Serum MPO levels were determined with sandwich ELISA kits on the basis of the manufacturer’s instructions (Bioassay Technology Laboratory, Shanghai, China) using Alisei Elisa Reader (Italy/Rome). Serum testosterone levels were measured in an autoanalyzer (Beckman Coulter, CA USA), an automated chemiluminescence immunoassay system.

KAVRAM SARIHAN K., YARDIMOĞLU YILMAZ M., ERALDEMİR F.C., YAZIR Y, & ACAR E. (2020). Protective effects of apocynin on damaged testes of rats exposed to methotrexate. Turkish Journal of Medical Sciences, 50(5), 1409-1420.

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Tissue Homogenization and Oxidative Biomarker Analysis

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The tissues were weighed and homogenized with a tissue homogenizer by adding 1/10 (weight/volume) phosphate-buffered saline (PBS) (0.1 M/pH 7.4). Homogenates were centrifuged at 3000×
gfor 15 min and the supernatants were separated and stored in Eppendorf tubes at –40 °C until analysis. MDA and GSH levels were measured spectrophotometrically using the 1240 UV mini spectrophotometer (SHIMADZU) instrument with the manual method [19,21]. MPO levels were analyzed with Bioassay (Shanghai, China) ELISA kit using Alisei Elisa Reader (Italy, Rome). Tissue protein assay was performed with the Lowry method with modification [22]. MDA, GSH, MPO results were given as a ratio to tissue protein.

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Salmonella Typhimurium Strain Cultivation

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Strains used in this study were derivatives of Salmonella enterica serovar Typhimurium strain LT2 [40] . Strain SV4280 was a gift of J. Casadesús. Except for the latter strain and for strain MA7224, all other strains were derived from MA3409, an LT2 derivative cured for the Gifsy-1 prophage [41] (link). The genotypes of the relevant strains used are listed in Table S1. Bacteria were cultured at 37°C in liquid media or in media solidified by the addition of 1.5% Difco agar. LB broth [42] (link) was used as complex medium. Carbon-free medium (NCE) [43] (link), supplemented with 0.2% glycerol or 0.2% lactose was used as minimal medium. Antibiotics (Sigma-Aldrich) were included at the following final concentrations: chloramphenicol, 10 µg ml⁻¹; kanamycin monosulphate, 50 µg ml⁻¹; sodium ampicillin 100 µg ml⁻¹; spectinomycin dihydrochloride, 80 µg ml⁻¹; tetracycline hydrochloride, 25 µg ml⁻¹. MacConkey agar plates containing 1% lactose [44] (link) were used to monitor lacZ expression in bacterial colonies. Liquid cultures were grown in New Brunswick gyratory shakers and growth was monitored by measuring the optical density at 600 nm with a Shimazu UV-mini 1240 spectrophotometer.

Yang Q., Figueroa-Bossi N, & Bossi L. (2014). Translation Enhancing ACA Motifs and Their Silencing by a Bacterial Small Regulatory RNA. PLoS Genetics, 10(1), e1004026.

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Characterizing Salmonella Typhimurium Strains

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Strains used in this study were all derived from Salmonella enterica serovar Typhimurium strain MA3409, a derivative of strain LT2 cured for the Gifsy-1 prophage (Figueroa-Bossi et al. 1997 (link)). The genotypes of strains used are listed in Supplemental Table S1. Bacteria were cultured at 37°C in liquid LB broth (Bertani 2004 (link)) or LB broth solidified by the addition of 1.5% Difco agar. Antibiotics (Sigma-Aldrich) were included at the following final concentrations: 10 µg/mL chloramphenicol, 50 µg/mL kanamycin monosulphate, 100 µg/mL sodium ampicillin, 80 µg/mL spectinomycin dihydrochloride, and 25 µg/mL tetracycline hydrochloride. LB plates containing 40 µg/mL 5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside (X-gal; from Sigma) or MacConkey agar plates containing 1% lactose (Macconkey 1905 (link)) were used to monitor lacZ expression in bacterial colonies. Liquid cultures were grown in New Brunswick gyratory shakers, and growth was monitored by measuring the optical density at 600 nm with a Shimazu UV mini 1240 spectrophotometer.

Figueroa-Bossi N., Schwartz A., Guillemardet B., D’Heygère F., Bossi L, & Boudvillain M. (2014). RNA remodeling by bacterial global regulator CsrA promotes Rho-dependent transcription termination. Genes & Development, 28(11), 1239-1251.

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Kinetics of Thrombin-Induced Fibrin Formation

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Thrombin-catalyzed fibrin polymerization was followed by measuring changes in turbidity at 350 nm at ambient temperature using an UVmini-1240 spectrophotometer (Shimazu, Tokyo, Japan), as described elsewhere [19] . Three parameters: the lag time, maximum slope, and absorbance change (ΔAbs) for 30 min, were obtained from the turbidity curves, as previously described [19] . The reactions were performed in triplicate for each sample.
The clottability (percentage of fibrinogen incorporated into a fibrin gel) of purified fibrinogens was determined as described before [20] .

Mukai S., Ikeda M., Takezawa Y., Sugano M., Honda T, & Okumura N. (2015). Differences in the function and secretion of congenital aberrant fibrinogenemia between heterozygous γD320G (Okayama II) and γΔN319-ΔD320 (Otsu I). Thrombosis research, 136(6).

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Antioxidant Capacity Evaluation of ABTS

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The quenching capacity of ABTS^•+ cation radicals was determined spectrophotometrically according to the methodology given by Re et al. [55 (link)]. In total, 1.8 mL of ABTS^•+ radical and 0.04 mL of buffer extract were measured into a tube. After 5 min, color measurement was carried out using a Mini 1240 UV spectrophotometer (Shimadzu, Kyoto, Japan) at 734 nm. The quenching ability of ABTS^•+ cation radicals was expressed as EC₅₀.

Cacak-Pietrzak G., Dziki D., Gawlik-Dziki U., Sułek A., Kalisz S, & Sujka K. (2021). Effect of the Addition of Dried Dandelion Roots (Taraxacum officinale F. H. Wigg.) on Wheat Dough and Bread Properties. Molecules, 26(24), 7564.

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Grape Berry Bioactive Compounds Analysis

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Total soluble solids (TSS), pH, and total skin anthocyanins were analyzed 0, 7, 14, 21, 28, 35, and 42 days after the beginning of treatments in five berries sampled from five different plants. Malic acid was measured on day 0, 7, 21, and 35. For that, grapes were manually separated into skin, pulp and seeds. TSS, pH, and malic acid were measured in the must extracted from the pulp: TSS using a desktop refractometer (Abbe Digital 315RS, Zuzi); malic acid with an enzymatic method (Enzytec™ L‐Malic Acid, Boehringer Mannheim/R‐Biopharm); and pH with a pH meter (Crison Instruments). In order to calculate the sugar content per berry, total sugars per berry mass were estimated from TSS according to the International Organization of Vine (OIV, 2016 ). The skins were lyophilized and, subsequently, ground with a batch ball mill (Retsch MM400). Total skin anthocyanins was measured according to the method described in Arrizabalaga et al. (2018 (link)). Briefly, 50 mg of sample were taken and 2 ml of methanol–HCL (0.1%) was added. The samples were stirred for 60 min and centrifuged at 4100g for 10 min at 4°C. The supernatant was taken and diluted in methanol–HCL (0.1%) to subsequently measure the absorbance in a mini‐1240 UV spectrophotometer (Shimadzu) at 536 nm. The calibration curve used was prepared with malvidin‐3‐O‐glucoside (Sigma‐Aldrich).

Pascual I., Antolín M.C., Goicoechea N., Irigoyen J.J, & Morales F. (2022). Grape berry transpiration influences ripening and must composition in cv. Tempranillo (Vitis vinifera L.). Physiologia Plantarum, 174(4), e13741.

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