Sensitized mice were intraperitoneally challenged with 0.9% sterile saline, OVA, or venom proteins with the quantities of OVA and venom previously described for a total injection volume of 500.0 μL per individual. Twenty-four hours after the challenge, peritoneal cavities were washed with PBS containing 0.1% heparin and the resulting suspensions were used for total and differential cell counting, using a Neubauer chamber and cytospun slides, respectively. The slides were stained with panoptic stain. The peritoneal exudates were harvested for chemokine quantification (Eotaxin - BDBiosciences) by ELISA according to the manufacturer’s protocol.
Eotaxin
Manufactured by BD
Sourced in United States
Eotaxin is a chemokine protein that plays a role in the recruitment and activation of eosinophils, a type of white blood cell. It functions in the regulation of eosinophilic inflammation. Eotaxin is used in laboratory research to study the mechanisms of eosinophil-mediated immune responses.
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Lab products found in correlation
Rantes, by RayBiotech (1 mentions)
Interleukin 4 (il 4), by BD (1 mentions)
Il 13, by R&D Systems (1 mentions)
Human ifn γ, by BD (1 mentions)
Human il 6, by BD (1 mentions)
Human il 10, by BD (1 mentions)
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Rantes, by BD (1 mentions)
Gm csf, by BD (1 mentions)
Hepes, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Rneasy mini kit, by Qiagen (1 mentions)
Dulbecco s modified eagle s medium dmem, by Lonza (1 mentions)
4 protocols using eotaxin
1
Allergic Response Analysis in Mice
2
Quantification of Allergy Biomarkers
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Concentrations of eotaxin, IL-4, IL-5 (BD Pharmingen, CA, USA), RANTES (RayBiotech Inc., GA), and IL-13 (R&D Systems, Minneapolis, MN) in BALF were quantified using sandwich EIA kits according to the manufacturer's instructions. Similarly, the serum level of total IgE was quantified with a commercially available EIA kit (BD Pharmingen, CA, USA).
3
Multiplex Cytokine Profiling in Plasma
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The Human IL-1ra, Human IL-6, Human IL-10, Human IL-13, Human Eotaxin, Human IFN-γ, Human SCF and Human TRAIL (BD Biosciences, San Jose, CA, United States) ELISA kits were used to measure plasma concentration of IL-1ra, IL-6, IL-10, IL-13, Eotaxin, IFN-γ, SCF and TRAIL, respectively. We used assay protocol and sample dilution in accordance with the manufacturers’ instructions.
4
Cytokine and Chemokine Profiling in Inflammatory Cells
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Dulbecco’s modified Eagle’s medium (DMEM) was obtained from Lonza (Verviers, Belgium), fetal bovine serum (FBS) from Biological Industries (Beit Haemek, Israel). Charcoal-stripped (steroid-free) FBS (csFBS), trypsin-EDTA, penicillin, streptomycin, HEPES, RT-PCR and immunofluorescence and Western Blot reagents, small interfering RNAs (siRNA), and all other transfection reagents were purchased from Life Technologies (Paisley, UK). Dexamethasone (Fortecortin) was obtained from Merck (Darmstadt, Germany) and amphotericin B and LPS (from Escherichia coli 0111:B4) from Sigma-Aldrich (St Louis, Missouri, USA). The RNeasy Mini kit was purchased from Qiagen Inc. (Valencia, California, USA). All antibodies were from Santa Cruz Biotechnology (Santa Cruz, California, USA), unless otherwise specified. RANTES, eotaxin, GM-CSF, and CXCL8 BD Cytometric Bead Array Flex Sets were from BD Biosciences (San Diego, California, USA), and IL-6 and CXCL8 DuoSet commercial enzyme-linked immunosorbent assay (ELISA) kits were from R&D Systems (Abingdon, UK).
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