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Hla abc w6 32

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The HLA-ABC (W6/32) is a monoclonal antibody used for the detection of human leukocyte antigen (HLA) class I molecules, which include HLA-A, HLA-B, and HLA-C. It is a research-use-only product intended for use in various immunoassay techniques, such as flow cytometry, immunohistochemistry, and immunoprecipitation.

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Lab products found in correlation

Cd44 im7, by Thermo Fisher Scientific (2 mentions) Lsrfortessa, by BD (1 mentions) Cd45 2d1, by Thermo Fisher Scientific (1 mentions) Mu5ubee, by Thermo Fisher Scientific (1 mentions) Cd105 sn6, by Thermo Fisher Scientific (1 mentions) Cd73 ad2, by Thermo Fisher Scientific (1 mentions) Cd31 wm59, by Thermo Fisher Scientific (1 mentions) Hla dr l243, by Thermo Fisher Scientific (1 mentions) Ccr7 3d12, by Thermo Fisher Scientific (1 mentions) Cxcr4 12g5, by Thermo Fisher Scientific (1 mentions) Streptavidin macs beads, by Miltenyi Biotec (1 mentions) Ficoll paque gradient, by GE Healthcare (1 mentions) Cd1d 51, by BioLegend (1 mentions) Navios flow cytometer, by Beckman Coulter (1 mentions) Facscanto 2, by Tree Star (1 mentions) Trastuzumab, by Genentech (1 mentions)

Close spelling variants of this product

HLA-ABC (17 citations) W6/32 (14 citations) Anti-HLA-ABC-APC (clone W6/32; (2 citations) PE-anti-HLA-ABC (clone W6/32, (2 citations) Anti-human HLA-ABC (clone W6/32) (2 citations) HLA-ABC (clone W6/32, (2 citations)

4 protocols using hla abc w6 32

1

Flow Cytometry Characterization of MSCs

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Fluorescence-activated cell sorting flow cytometry analysis was performed using CD29 (TS2/16; eBioscience, San Diego, CA, United States), CD44 (IM7; eBioscience), CD105 (SN6; eBioscience), CD90 (5E10, eBioscience), CD73 (AD2, eBioscience), CD45 (2D1; eBioscience), CD31 (WM-59; eBioscience), CD34 (4H11; eBioscience), HLA-ABC (W6/32; eBioscience), HLA-DR (L243; eBioscience), C-C chemokine receptor type 1 (CCR1; 5F10B29; BioLegend, San Diego, CA, United States), CCR2 (K036C2; BioLegend), CCR7 (3D12; eBioscience), C-X-C chemokine receptor type 4 (CXCR4; 12G5; eBioscience) and CXCR5 (MU5UBEE; eBioscience) antibodies to confirm that the MSC phenotype was maintained after expansion in the culture. The samples were incubated with antibodies against each surface marker for 30 min, and this treatment was followed by fluorescence-activated cell sorting. Flow cytometry analysis was performed on a LSRFortessa (BD Pharmingen, San Diego, CA, United States).
Song Y., Lim J.Y., Lim T., Im K.I., Kim N., Nam Y.S., Jeon Y.W., Shin J.C., Ko H.S., Park I.Y, & Cho S.G. (2020). Human mesenchymal stem cells derived from umbilical cord and bone marrow exert immunomodulatory effects in different mechanisms. World Journal of Stem Cells, 12(9), 1032-1049.
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2

Isolation and Cloning of CLA+ T Cells

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Blood was obtained from healthy volunteers after informed consent and the study was approved by the Ethics Committee of the National University of Singapore (NUS-IRB 10-250). Peripheral blood mononuclear cells were separated by ficoll-paque gradient (GE Healthcare, Little Chalfont, UK) and enriched for those expressing cutaneous lymphocyte-associated antigen (CLA) using CLA-specific biotin-conjugated mAbs (HECA-542) and streptavidin-MACS beads (Miltenyi Biotec, Bergisch Gladbach, Germany). CLA-enriched cells were co-cultured with autologous moDCs in RPMI containing 5% autologous serum and 5 units/ml IL-2. After 12 days the cells were re-stimulated with autologous moDC in medium supplemented with 50 units/ml IL-2 and after another 12 days cloned by limiting dilution. Clones were screened with allogenic moDC in conjunction with mAbs specific for HLA-DR (L243), HLA-ABC (W6/32, both eBioscience, San Diego, CA), CD1a (OKT6), CD1b (BCD1b3.1), CD1c (F10/21A3.1 all purified in house) and CD1d (51.1, Biolegend, San Diego, CA).
Betts R.J., Perkovic A., Mahapatra S., Del Bufalo A., Camara K., Howell A.R., Martinozzi Teissier S., De Libero G, & Mori L. (2017). Contact sensitizers trigger human CD1-autoreactive T-cell responses. European journal of immunology, 47(7), 1171-1180.
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3

Multiparameter Flow Cytometry Analysis

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Monoclonal antihuman-EpCAM (clone: 9C4; manufacturer: Biolegend, fluorescent protein: APC), -CD133 (293C3, Milteny Biotech, PE), -CD44 (IM7, eBioscience, PE), -CD24 (eBioSN3, eBioscience, PE), -HLA-ABC (W6/32, eBioscience, PE), -CD95 (DX2, Thermofisher, APC), -FasL (NOK-1, Biolegend, PE), and -PD-L1 (MIH1, Thermofisher, PE) as well as mouse-IgG1 antibodies as isotype controls (P3.6.2.8.1, eBioscience, APC; MOPC-173, Becton Dickson, PE) were used for flow cytometry. Collected cells were first washed with PBS + 2% FCS (FACS buffer), stained with the fluorochrome-conjugated monoclonal antibodies, and incubated for 15 min at room temperature. Upon washing to remove unbound reagents, cells were resuspended in FACS buffer, acquired on a NaviosTM flow cytometer (Beckman Coulter Inc., Brea, CA, USA), and analyzed with Cytometry List Mode Data Acquisition and Analysis Software (Beckman Coulter Inc.).
Schönberger S., Kraft D., Nettersheim D., Schorle H., Casati A., Craveiro R.B., Mohseni M.M., Calaminus G, & Dilloo D. (2020). Targeting EpCAM by a Bispecific Trifunctional Antibody Exerts Profound Cytotoxic Efficacy in Germ Cell Tumor Cell Lines. Cancers, 12(5), 1279.
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4

Flow Cytometric Analysis of HER2/EGFR Proteins

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HER2/neu epitopes were detected by mAb TA-1 (Calbiochem), Trastuzumab (Genentech), 7.16.4 (Calbiochem), N12 or N29 (hybridoma cell lines were generous gifts of Dr. Yosef Yarden, Weissman Institute, Israel). mAb to human EGFR (528, Santa Cruz Biotech), HER3 (SGP1, eBioscience) and HLA-ABC (W6/32, eBioscience) were used as indicated. Phycoerythrin (PE)-conjugated goat anti-mouse or anti-human IgG was the secondary antibody (Jackson ImmunoResearch). Flow cytometric analysis was performed using FACS Canto II and data analyzed with FlowJo (Tree Star).
To measure antibody level in immune sera, mouse or feline sera were incubated with 3T3 cells engineered to express the designated antigen and detected by PE-conjugated anti-mouse or feline IgG secondary antibody (SantaCruz). Mouse antibody concentrations were extrapolated from a standard curve of HER2 mAb TA-1. Feline antibody titers were determined by serial dilution until binding was no longer detected above that of the isotype control.
Gibson H., Veenstra J., Jones R., Vaishampayan U., Sauerbrey M., Bepler G., Lum L., Reyes J., Weise A, & Wei W.Z. (2015). Induction of HER2 Immunity in Outbred Domestic Cats by DNA Electrovaccination. Cancer immunology research, 3(7), 777-786.
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