Chromafil
Chromafil is a line of single-use syringe filters manufactured by Macherey-Nagel. These filters are designed to remove particulates and clarify liquid samples before analysis or further processing. The core function of Chromafil filters is to provide efficient filtration of small sample volumes.
Lab products found in correlation
27 protocols using chromafil
Comprehensive Characterization of Nanoparticles
Micropollutant Transformation in Wastewater Treatment
Quantification of Bioactive Compounds in Lamium album
Soil Pore Water Extraction Protocol
Nanoparticle Production Yield Calculation
Drug loading (DL) and entrapment efficiency (EE) were determined by HPLC analysis. Ten milligrams of each batch of nanoparticles were dissolved in 5 mL mobile phase and sonicated for 5 min into an ultrasonic bath (Sonorex Bandelin electronic, Berlin, Germany) until dissolution of the particles and extraction of DRB was achieved. The blend was then centrifuged at 5000 rpm for 15 min and filtered (0.22 µm, Chromafil®, Macherey-Nagel, Düren, Germany). The supernatant was recovered and the concentration of DRB was determined via HPLC (UltiMate 3000, Thermo Scientific, Waltham, MA, USA) under the following conditions: a 254 nm wavelength; a 5 µL injection volume; an Inertsil® ODS-3 HPLC column of 5 µm, 150 × 4.6 mm (GL Sciences, Tokyo, Japan); a 1 mL/min flow rate; a 25 °C oven temperature; a 20 °C sampler temperature; and mobile phase: 0.29% sodium dodecyl sulfate in 0.2% aqueous solution of phosphoric acid:acetonitril (1:1). The drug loading and entrapment efficiency were calculated using Equations (2) and (3), respectively.
In Vitro Drug Release from Casein Nanoparticles
Enzymatic Synthesis of 3-Ketodihydrosphingosine
Initially, samples containing 0.1 M HEPES pH 7.5, 2.5 mM MgCl2, 0.5 mM TCEP, 0.5 mM ATP, 20 μM CoA, 0.5 mM palmitic acid (50 mM stock solution in DMSO, final DMSO concentration 1% [vol/vol]), 5 mM serine, 20 μg/mL FadD, and 60 μg/mL SPT were incubated at 37°C for different time points. For extraction of 3-ketodihydrosphingosine, to confirm its formation, an equal volume of methanol was added after 2 h. The sample was filtered through a 0.2 μm PTFE filter (Chromafil, Macherey-Nagel) and analyzed by HPLC-HRMS. In all other cases, the reaction was stopped by adding ammonium molybdate (see PPi assay). Later, samples with a final DMSO concentration of 10% (vol/vol) were set up. For samples containing inhibitors (dissolved in DMSO), the final DMSO concentration was also kept at 10% (vol/vol). For blank samples, CoA was replaced with water and for samples measuring FadD activity alone SPT was replaced with buffer C. For measurements with different serine concentrations, appropriate stock solutions were used to set up the reactions. The PPi assay was performed as described before (23 (link)). All measurements were done including technical and biological triplicates.
Screening Fungal Cultures for Depsipeptide Analogues
Mycotoxin Extraction and Quantification
Characterization of Synthesized Nanoparticles
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