All mouse experiments were approved under the Vanderbilt University Institutional Animal Care and Use Committee. Mice were housed in 12hr light/dark cycles in temperature and humidity-controlled facilities with ad-libitum access to chow diet and water. Transgenic CETP were purchased from the Jackson Laboratories (C57BL/6-Tg(CETP)UCTP20Pnu/J, Stock No: 001929). Non-transgenic littermates were used as WT controls. Male mice used in this study were aged 3–4 months old to ensure complete sexual development. All animals were sacrificed between 8 and 11 am to minimize circadian variation in gene expression. Adeno-associated virus serotype 8 (AAV8) containing short-hairpin RNA (shRNA) specific to mouse androgen receptor was purchased from Vector Biolabs (shADV-252885) with the shRNA under control of a U6 promoter and a GFP reporter under control of a CMV promoter. AAV8 has been shown to have tropism for liver (Tenney et al., 2014 (link)). 1.8E12 GC/mouse was injected intravenously under brief isoflurane anesthesia (5%). Euthanasia was achieved using carbon dioxide and confirmed with cervical dislocation.
Shadv 252885
Manufactured by Vector Biolabs
ShADV-252885 is a laboratory equipment product offered by Vector Biolabs. It serves as a device for specialized applications in the field of biological research and analysis. The core function of this equipment is to perform specific tasks within the scope of its design and capabilities.
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Lab products found in correlation
2 protocols using shadv 252885
1
Modulating Androgen Receptor in Mice
2
Androgen Receptor Knockdown in Transgenic CETP Mice
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All mouse experiments were approved under the Vanderbilt University Institutional Animal Care and Use Committee. Mice were housed in 12 h light/dark cycles in temperature and humidity‐controlled facilities with ad libitum access to chow diet and water. Transgenic CETP were purchased from the Jackson Laboratories (C57BL/6‐Tg(CETP)UCTP20Pnu/J, Stock No: 001929). Non‐transgenic littermates were used as WT controls. Male mice used in this study were aged 3–4 months old to ensure complete sexual development. All animals were sacrificed between 8 and 11 am to minimize circadian variation in gene expression. Adeno‐associated virus serotype 8 (AAV8) containing short‐hairpin RNA (shRNA) specific to mouse androgen receptor (AR) was purchased from Vector Biolabs (shADV‐252,885, Malvern, PA) with the shRNA under control of a U6 promoter and a green fluorescence protein (GFP) reporter under control of a cytomegalovirus (CMV) promoter. AAV8 has been shown to have tropism for liver (Tenney et al., 2014 ). 1.8E12 GC/mouse was injected intravenously under brief isoflurane anesthesia (5%). Euthanasia was achieved using carbon dioxide and confirmed with cervical dislocation.
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