Tcs sp8 sted 3x confocal laser scanning microscope

Scanning electron microscopy (SEM) images were obtained with a GeminiSEM 500. Dried samples were placed on the conductive adhesive and sprayed with gold. Transmission electron microscopy (TEM) was performed on an H-7650 device to observe the size and morphology of the samples. The surface element composition of the samples was obtained using an Oxford energy-dispersive spectrometer (EDS). The magnetic property of the samples was measured using an MPMS-SQUID VSM-094 vibrating sample magnetometer (VSM). Fluorescence images of samples were obtained using a Leica TCS SP8 STED 3X confocal laser scanning microscope (CLSM). A 6100 X-ray diffractometer (XRD) was used to characterize the crystal structure of entrapped glucose oxidase. Thermal gravimetric analysis (TGA) of entrapped glucose oxidase was performed with an SDTQ600 thermal gravimetric analyzer (New Castle, DE, USA).

TEM images were obtained on JEOL JEM2010 operated at 200 kV. TGA was conducted on a METTLER TOLEDO’s SDTA851e simultaneous thermal analyzer, collecting the thermogravimetric change data from 25 °C to 800 °C in a continuous flow of nitrogen for ~5 mg samples. Powder XRD patterns were recorded using a Rigaku Corporation’s Ultima IV X-ray diffractometer. FTIR spectra were collected using the iS50 Fourier transform infrared spectrometer from Nicolet. Particle size distributions were obtained using NANO ZS360 NANO particle size. STEM and EDX mapping experiments were performed on a FEI Probe Cs-corrected Titan operating at 200 kV. Confocal microscopy images were recorded using a Leica TCS SP8 STED 3X confocal laser scanning microscope to determine the presence and spatial location of the fluorophore-tagged enzymes in the multi-shelled (hollow) ZIF-8 MOFs. Ultraviolet-visible absorption spectra were collected using Agilent Cary 60 spectrophotometer. The nitrogen gas adsorption–desorption was measured at 77 K on a Quantachrome Autosorb-iQ-MP volumetric gas adsorption analyzer. Gas chromatography (GC) was conducted on Shimadzu’s GC-2010 pro.

B16-F10 cells were cultured in RMPI 1640 medium containing 10% FBS and 1% antibiotics. ZnO nanocrystals are dispersed in DMSO. Subsequently, 1 mL of the suspension was added to the culture medium, and then the cells were placed in an incubator at 37 ^oC in 5% CO₂ for 12 h. The cells were washed for three times with warm phosphate buffer to remove excess the nanocrystals. Then, the cells were fixed on a glass slide to take pictures of one-photon and two-photon cell imaging using a Leica TCS SP8 STED 3X laser scanning confocal microscope.

The microstructures of all the CNDs were characterized by transmission electron microscopy (TEM, JSM-6700), and X-ray diffraction (XRD) patterns were collected with a Bruker D8 Discover (Germany) X-ray diffractometer. For the measurement of the fluorescence and phosphorescence of the samples, the spectra were characterized using a Hitachi F-7000 PC spectrophotometer. The lifetimes were measured using an FLS-1000 spectrometer equipped with a microsecond flash lamp. The absolute PL QYs were measured using an absolute photoluminescence quantum yield FLS-1000 equipped with an integrating sphere under ambient conditions. UV–vis absorption spectra were performed using a Hitachi UH4150 UV–vis spectrophotometer. The FTIR spectra of the samples were recorded using a Bruker VERTEX-70 FTIR spectrometer. The fluorescence imaging of bacteria was performed by using a Leica TCS SP8 STED 3X laser scanning confocal microscope.