For staining of SKL cells (Sca-1+ c-Kit+ Lin–), VSELs (Sca-1+ Lin– CD45–), MSCs (Lin– CD45– CD31– CD90+), and EPCs (Lin– CD45– CD31+) the following monoclonal antibodies were used: FITC–anti-CD117 (also known as c-Kit, clone 2B8; BioLegend, San Diego, CA, USA) and PE–Cy5–antimouse Ly-6 A/E (also known as Sca-1, clone D7; eBioscience, San Diego, CA, USA). All anti-mouse lineage marker antibodies, including anti-CD45R (also known as B220, clone RA3-6B2), anti-Ter-119 (clone TER-119), anti-CD11b (clone M1/70), anti-T cell receptor β (clone H57–597), anti-Gr-1 (clone RB6-8C5), anti-TCRγδ (clone GL3), and anti-CD45 (clone 30-F11), conjugated with PE; anti-CD31 (clone MEC 13.3), conjugated with APC; and anti-CD90.2 (clone 30-H12), conjugated with BV510, were purchased from BD Biosciences. Staining was performed in RPMI-1640 medium containing 2% FBS. All monoclonal antibodies were added at saturating concentrations, and the cells were incubated for 30 min on ice, washed twice, and analyzed with an LSR II flow cytometer (BD Biosciences) [15 (link), 17 (link)].
Anti tcrγδ clone gl3
Manufactured by BD
Sourced in United States
Anti-TCRγδ (clone GL3) is a monoclonal antibody that recognizes the T cell receptor gamma delta (TCRγδ). It can be used for the identification and enumeration of TCRγδ+ T cells by flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Fitc anti cd117, by BioLegend (5 mentions)
Anti cd45 clone 30 f11, by BD (5 mentions)
Lsrii flow cytometer, by BD (5 mentions)
Pe cy5 anti mouse ly 6 a e, by Thermo Fisher Scientific (4 mentions)
Anti gr 1 clone rb6 8c5, by BD (4 mentions)
Anti cd90.2 clone 30 h12, by BD (2 mentions)
Anti cd31 clone mec13, by BD (2 mentions)
Anti cd45r, by BD (2 mentions)
Pe cy5 anti mouse ly 6a e sca 1 clone d7, by Thermo Fisher Scientific (2 mentions)
Bv510, by BD (1 mentions)
C kit, by BioLegend (1 mentions)
Facsverse cytometer, by BD (1 mentions)
Anti ter119 clone ter 119, by BD (1 mentions)
Anti cd34 clone ram34, by BD (1 mentions)
Anti cd11b clone m1 70, by BD (1 mentions)
7 protocols using anti tcrγδ clone gl3
1
Multiparametric Flow Cytometry for Stem Cell Subsets
2
Multilineage Stem Cell Characterization
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For staining of Lin−/Sca-1+/c-Kit+ (SKL cells), Lin−/Sca-1+/CD45+ (HSCs), Sca-1+/Lin−/CD45− (VSELs), Lin−/CD45−/CD31+ (EPCs), and Lin−/CD45−/CD31−/CD90+ (MSCs), the following monoclonal antibodies were used: FITC–anti-CD117 (also known as c-Kit, clone 2B8; BioLegend, San Diego, CA, USA) and PE–Cy5–anti-mouse Ly-6 A/E (also known as Sca-1, clone D7; eBioscience, San Diego, CA, USA). All anti-mouse lineage marker antibodies, including anti-CD45R (also known as B220, clone RA3-6B2), anti-Ter-119 (clone TER-119), anti-CD11b (clone M1/70), anti-T cell receptor β (clone H57–597), anti-Gr-1 (clone RB6-8C5), anti-TCRγδ (clone GL3), and anti-CD45 (clone 30-F11), conjugated with PE; anti-CD31 (clone MEC 13.3), conjugated with APC; and anti-CD90.2 (clone 30-H12), conjugated with BV510, were purchased from BD Biosciences. Staining was performed in RPMI-1640 medium containing 2% FBS. All monoclonal antibodies were added at saturating concentrations, and the cells were incubated for 30 min on ice, washed twice, and analyzed with a FACSVerse cytometer (BD Biosciences) [26 (link)].
3
Immunophenotyping of Hematopoietic Stem Cells
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The following monoclonal antibodies were used to perform staining of Lin–/Sca-1+/c-Kit+ (SKL) cells and Lin–/Sca-1+/CD45+ (HSCs): FITC–anti-CD117 (also known as c-Kit, clone 2B8; BioLegend) and PE-Cy5-anti-mouse Ly-6 A/E (also known as Sca-1, clone D7; eBioscience, San Diego, CA, USA). All anti-mouse lineage markers (Lin) were purchased from BD Biosciences: anti-CD45R/B220 (clone RA3-6B2), anti-Ter-119 (clone TER-119), anti-CD11b (clone M1/70), anti-T-cell receptor β (clone H57-597), anti-Gr-1 (clone RB6-8C5), anti-TCRγδ (clone GL3) and anti-CD45 (clone 30-F11) and conjugated with PE as described.24 (link), 29 (link), 31 (link), 32 (link) All monoclonal antibodies were added at saturating concentrations, and the cells were then incubated for 30 min on ice, washed twice, resuspended in RPMI-1640 plus 2% fetal bovine serum, and analyzed with an LSR II flow cytometer (BD Biosciences, San Diego, CA, USA).
4
Identification of Hematopoietic Stem and Progenitor Cells
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The following monoclonal antibodies were employed to perform analytical staining of Lin−/Sca-1+/c-kit+ (SKL cells) and Lin−/Sca-1+/CD45+ (HSCs): fluorescein isothiocyanate (FITC) anti-CD117 (c-kit) (clone2B8; BioLegend, San Diego, CA) and phycoerythrin (PE)-Cy5 anti-mouse Ly-6A/E (Sca-1) (clone D7; eBioscience™, San Diego, CA). All anti-mouse lineage markers (Lin) were conjugated with PE and purchased from BD Biosciences: anti-CD45R/B220 (clone RA3-6B2); anti-Ter-119 (clone TER-119); anti-CD11b (clone M1/70); anti-T-cell receptor β (TCRβ; clone H57-597); anti-Gr-1 (clone RB6-8C5); anti-TCRγδ (clone GL3); and anti-CD45 (clone 30-F11). All monoclonal antibodies were added at saturating concentrations, and the cells were then incubated for 30 min on ice, washed twice, resuspended in RPMI-1640 plus 2% fetal bovine serum, and analyzed with an LSR II flow cytometer (BD, San Diego, CA, USA) [11 (link)].
5
Immunophenotyping of Mouse Hematopoietic Stem Cells
6
Multiparametric Immunophenotyping of Murine Stem Cells
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BMNC staining was performed in medium containing 2 % fetal bovine serum (FBS). All monoclonal antibodies (mAbs) were added at saturating concentrations and the cells were incubated for 30 min on ice, washed twice, resuspended in staining solution at a concentration of 5 × 106 cells/ml, and subjected to analysis using an LSR II (Becton Dickinson, Mountainview, CA). The following anti-mouse Abs were used to detect fluorescein isothiocyanate (FITC)-anti-CD117 (c-Kit) (clone 2B8; BioLegend, San Diego, CA) and Phycoerythrin (PE)-Cy5 anti-mouse Ly-6A/E (Sca-1) (clone D7; eBioscience™, San Diego, CA). All anti-mouse lineage markers (Lin) were conjugated by PE and purchased from BD Biosciences: anti-CD45R/B220 (clone RA3-6B2); anti-Gr-1 (clone RB6-8C5); anti-T-cell receptor β (TCRβ; clone H57-597); anti-TCRγδ (clone GL3); anti-CD11b (clone M1/70); anti-Ter-119 (clone TER-119); and anti-CD34 (clone RAM34) as described [8 –10 (link)].
7
Identification of Hematopoietic Stem/Progenitor Cells
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For the staining of Lin–/Sca-1+/c-Kit+ (SKL) cells, the following monoclonal antibodies were used: FITC–anti-CD117 (also known as c-Kit, clone 2B8; BioLegend, San Diego, CA, USA), PE–Cy5–anti-mouse Ly-6 A/E (also known as Sca-1, clone D7; eBioscience, San Diego, CA, USA), and anti-mouse lineage-marker antibodies, including anti-CD45R (also known as B220, clone RA3-6B2), anti-Ter-119 (clone TER-119), anti-CD11b (clone M1/70), anti-T cell receptor β (clone H57-597), anti-Gr-1 (clone RB6-8C5), and anti-TCRγδ (clone GL3) conjugated with PE (BD Biosciences, San Jose, CA, USA). Staining was performed in RPMI-1640 medium containing 2% FBS. All monoclonal antibodies were added at saturating concentrations, and the cells were incubated for 30 min on ice, washed twice, and analyzed using an LSRII flow cytometer (BD Biosciences, San Jose, CA, USA) [4 (link), 7 (link), 26 (link)].
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