AAV viruses used in this study were as follows: AAV1.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH (UPenn AV-1–20298P), AAV1.hysn.hChR2(H134R)-eYFP.WPRE.hGH (UPenn AV-26973P), AAV1.EF1a.DIO.eYFP.WPRE.hGH (Upenn AV-1–27056), AAVrg.CAG.GFP (Addgene 37825-AAVrg), AAVrg.CAG.tdTomato (Addgene 59462-AAVrg). Additional viral constructs were assembled for Cre-dependent expression of a reporter under the control of the Dlx5/6 enhancer: AAV-Dlx-Flex-GFP (Addgene #83900) and AAV-Dlx-Flex-ChR2-mCherry (Dimidschstein et al., 2016 (link)). These constructs take advantage of the double-floxed inverted system, in which two consecutive and incompatible lox-sites are placed both in 5' and 3' of the reversed coding sequences of the viral reporter, restricting expression to Cre-expressing interneurons.
Aav dlx flex gfp
Manufactured by Addgene
The AAV-Dlx-Flex-GFP is a plasmid vector designed for the expression of GFP (green fluorescent protein) in a Cre-dependent manner. It contains the Dlx promoter, which drives the expression of GFP in a subset of neurons. The vector is packaged in an adeno-associated virus (AAV) for efficient delivery and transduction into target cells.
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Lab products found in correlation
2 protocols using aav dlx flex gfp
1
Cre-Dependent AAV Viruses for Interneuron Targeting
2
Cre-dependent Expression of Viral Reporters
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Experiments used wild-type and transgenic mice of either sex in a C57BL/6J background (all breeders from Jackson Labs). Homozygote male breeders (PV-Cre = JAX 008069, SOM-Cre = JAX 013044, CCK-Cre = JAX 012706) were paired with female wild-type or Additional viral constructs were assembled for Cre-dependent expression of a reporter under the control of the Dlx5/6 enhancer: AAV-Dlx-Flex-GFP (Addgene #83900) and AAV-Dlx-Flex-ChR2-mCherry (Dimidschstein et al., 2016) . These constructs take advantage of the double-floxed inverted system, in which two consecutive and incompatible lox-sites are placed both in 5' and 3' of the reversed coding sequences of the viral reporter, restricting expression to interneurons.
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