Spike expressors of human coronaviruses SARS-CoV-2, SARS-CoV-1, MERS-CoV, OC43, NL63 and 229E were reported elsewhere (Hoffmann et al., 2020 (link); Hoffmann et al., 2013 (link); Hofmann et al., 2005 (link); Park et al., 2016 (link); Prevost et al., 2020 (link)). Expressors of HKU1 Spike and SARS-CoV-2 S2 N-His were purchased from Sino Biological. Using the standard calcium phosphate method, 10 μg of Spike expressor and 2 μg of a green fluorescent protein (GFP) expressor (pIRES2-eGFP) was transfected into 2 × 106 293T cells. At 48 hours post transfection, 293T cells were stained with CV3-1 and CV3-25 antibodies (5μg/mL), using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls. Alexa Fluor-647-conjugated goat anti-human IgG (H+L) Abs (Invitrogen) and goat anti-mouse IgG (H+L) Abs (Invitrogen) were used as secondary antibodies. The percentage of transfected cells (GFP+ cells) was determined by gating the living cell population based on the basis of viability dye staining (Aqua Vivid, Invitrogen). Samples were acquired on a LSRII cytometer (BD Biosciences) and data analysis was performed using FlowJo v10 (Tree Star).
Goat anti mouse igg h l abs
Manufactured by Thermo Fisher Scientific
Goat anti-mouse IgG (H+L) Abs is a lab equipment product that is used as a secondary antibody. It recognizes and binds to the heavy and light chains of mouse immunoglobulin G (IgG) antibodies. This product can be used in various immunoassay techniques, such as ELISA, Western blotting, and immunohistochemistry, to detect and quantify mouse IgG in samples.
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Lab products found in correlation
2 protocols using goat anti mouse igg h l abs
1
Quantifying SARS-CoV Spike Expression
2
Detecting Coronavirus Spike Protein Expression
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Spike expressors of human coronaviruses SARS-CoV-2, SARS-CoV-1, MERS-CoV, OC43, NL63 and 229E were reported elsewhere (Hoffmann et al., 2020 (link); Hoffmann et al., 2013 (link); Hofmann et al., 2005 (link); Park et al., 2016 (link); Prévost et al., 2020 (link)). Expressors of HKU1 Spike and SARS-CoV-2 S2 N-His were purchased from Sino Biological. Using the standard calcium phosphate method, 10 μg of Spike expressor and 2 μg of a green fluorescent protein (GFP) expressor (pIRES2-eGFP) was transfected into 2 × 106 293T cells. At 48 h post transfection, 293T cells were stained with CV3-1 and CV3-25 antibodies (5 μg/mL), using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls. Alexa Fluor-647-conjugated goat anti-human IgG (H+L) Abs (Invitrogen) and goat anti-mouse IgG (H+L) Abs (Invitrogen) were used as secondary antibodies. The percentage of transfected cells (GFP+ cells) was determined by gating the living cell population based on the basis of viability dye staining (Aqua Vivid, Invitrogen). Samples were acquired on a LSRII cytometer (BD Biosciences) and data analysis was performed using FlowJo v10 (Tree Star).
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