Xenograft experiments were performed in agreement with national guidelines and approved by the ethical committee of Animal Welfare Office of Italian Work Ministry and conform to the legal mandates and Italian guidelines for the care and maintenance of laboratory animals. 6–8 weeks old male SCID-bg/bg mice (Charles River Laboratories International, Inc., Wilmington, MA, USA) were injected subcutaneously (s.c.), both in the right and left lateral flanks, with cells mixed in a 1:1 volume ratio with Matrigel, in a final volume of 200 μl. Animals were monitored, tumour size was measured by a caliper and tumour volumes determined by the length (L) and the width (W): V = (LW2)/2.
Scid bg bg mice
Manufactured by Charles River Laboratories
Sourced in United States
SCID)-bg/bg mice are a strain of immunodeficient mice developed for use in biomedical research. These mice lack functional T and B cells, resulting in a severely compromised immune system. The black coat color is due to the bg (beige) mutation. The SCID)-bg/bg genotype makes these mice suitable as a model for evaluating the effects of various treatments or interventions in the absence of adaptive immune responses.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using scid bg bg mice
1
Xenograft Tumor Induction in SCID Mice
2
Melanoma Metastasis Model in SCID Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Experiments were conducted in accordance with national guidelines and were approved by the ethics committee of the Animal Welfare Office of the Italian Work Ministry and conformed to the legal mandates and Italian guidelines for the care and maintenance of laboratory animals. 2 × 106 Hs294T melanoma cells either treated with Etoposide or Etoposide plus Bevacizumab and suspended in 0.2 ml of PBS were injected into the lateral tail vein of six- to eight-week old female SCID-bg/bg mice (Charles River Laboratories International, Wilmington, MA, USA) (five animals per group). Animals were monitored every 3 days and sacrificed at different time points. Lungs were inspected for metastatic nodules with the help of a dissecting microscope, and then fixed overnight in 5% formalin in PBS for histological analysis.
3
Adoptive T-cell Therapy for Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The animals were randomized before cancer cell injection. Twelve six- to eight-week old male severe combined immunodeficient (SCID)-bg/bg mice (Charles River Laboratories International) were subcutaneously injected with 1×106 PC3 cells. The mice were monitored daily until measurable tumors were formed. Six mice were intratumorally injected with 1×106 T2Kb cells (previously activated with PC3 cell-conditioned media for 16h) preloaded with 10 μg/ml pOVA for an additional 4h. Six control mice were injected with vehicle (PBS). The injection volume was 100 μl. After 4h from the injection, 5x106 OT-I lymphocytes were injected into the tumor mass of all mice. The treatment was repeated every four days for a total of four times. Tumors were measured with calipers and the volumes were determined using the following formula: V = (Length × Width2)/2.
4
In vivo Xenograft Model of Prostate Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In vivo experiments were performed in accordance with national guidelines and approved by the ethical committee of Animal Welfare Office of Italian Work Ministry and conform to the legal mandates and Italian guidelines for the care and maintenance of laboratory animals. 6- to 8-week-old male severe combined immunodeficient (SCID)-bg/bg mice (Charles River Laboratories International) were injected s.c. with 1×106 PC3 cells plus 0.5×106 CAFs, both in the right and left lateral flanks. Mice received repeated courses of intra-peritoneal saline solution (control mice) or 100 μg/kg of ZA, once a week for 6 weeks. Animals (5 per group) were monitored daily. Tumour size was measured every 2 to 3 days by a caliper; tumor volumes were determined by the length (L) and the width (W): V = (LW2)/2. Mice were sacrificed after 6 weeks. Lungs were inspected for metastatic nodules by histological analyses.
5
SCID-bg/bg Mouse Xenograft Model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In vivo experiments were conducted in accordance with national guidelines and approved by the ethical committee of Animal Welfare Office of Italian Work Ministry and conform to the legal mandates and Italian guidelines for the care and maintenance of laboratory animals. Six-to 8-week-old male severe combined immunodeficient (SCID)-bg/bg mice (Charles River Laboratories International) were s.c. injected with 1 × 10 6 PC3 cells or mixed with 0.5 × 10 6 of fibroblasts for co-injection. For cell tracking and mitochondria transfer experiments, PC3 TdTomato and MitoGFP fibroblasts were used.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!