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Essential 8

Manufactured by Selleck Chemicals
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The Essential 8 is a laboratory equipment product designed for general use in scientific research and analysis. It serves as a multi-purpose tool, enabling various experimental procedures and data collection activities within a controlled laboratory environment.

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Lab products found in correlation

Rock inhibitor y 27632, by Selleck Chemicals (2 mentions) Matrigel, by Corning (2 mentions) Essential 8, by Thermo Fisher Scientific (2 mentions) Sb431542, by Selleck Chemicals (2 mentions) Ldn193189, by Selleck Chemicals (2 mentions) Essential 6, by Thermo Fisher Scientific (1 mentions) Essential 8 medium, by Thermo Fisher Scientific (1 mentions) Y 27632, by Selleck Chemicals (1 mentions)

Close spelling variants of this product

Essential 8 medium (2 citations)

3 protocols using essential 8

1

Directed neural induction of human iPSCs

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Neural induction of human iPSCs was performed using the dual SMAD inhibition paradigm6 (link),21 (link). Engineered CRISPRi-iPSCs62 (link) were grown in Essential 8 (Thermo Fisher Scientific) media on Matrigel (Corning) to 80% confluency. Cells were rinsed with DPBS and dissociated with Accutase (StemPro). After centrifugation at 300 × g for 3 minutes and resuspension in Essential 8 media with 10 μM Y-27632 ROCK inhibitor (Selleckchem), cells were replated at a density of 250,000 cells/cm2 overnight at 37° C. The next day (D0), cells were rinsed with DPBS and changed to neural induction media, which consisted of Essential 6 media (Thermo Fisher Scientific) with freshly-added SMAD inhibitors 500 nM LDN193189 (Selleckchem) and 10 μM SB431542 (Selleckchem). Media was replaced every 2 days until the endpoint of interest, as described21 (link).
Wu D., Poddar A., Ninou E., Hwang E., Cole M.A., Liu S.J., Horlbeck M.A., Chen J., Replogle J.M., Carosso G.A., Eng N.W., Chang J., Shen Y., Weissman J.S, & Lim D.A. (2022). Dual genome-wide coding and lncRNA screens in neural induction of induced pluripotent stem cells. Cell Genomics, 2(11), 100177.
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2

Neural Induction of iPSCs using CRISPR

Cited 3 times
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Neural induction of human iPSCs was performed using the dual SMAD inhibition paradigm.6 (link),21 (link) Engineered CRISPRi-iPSCs62 (link) were grown in Essential 8 (Thermo Fisher Scientific) media on Matrigel (Corning) to 80% confluency. Cells were rinsed with DPBS and dissociated with Accutase (StemPro). After centrifugation at 300 x g for 3 min and resuspension in Essential 8 media with 10 μM Y-27632 ROCK inhibitor (Selleckchem), cells were replated at a density of 250,000 cells/cm2 overnight at 37°C. The next day (D0), cells were rinsed with DPBS and changed to neural induction media, which consisted of Essential 6 media (Thermo Fisher Scientific) with freshly-added SMAD inhibitors 500 nM LDN193189 (Selleckchem) and 10 μM SB431542 (Selleckchem). Media was replaced every 2 days until the endpoint of interest, as described.21 (link)
Wu D., Poddar A., Ninou E., Hwang E., Cole M.A., Liu S.J., Horlbeck M.A., Chen J., Replogle J.M., Carosso G.A., Eng N.W., Chang J., Shen Y., Weissman J.S, & Lim D.A. (2022). Dual genome-wide coding and lncRNA screens in neural induction of induced pluripotent stem cells. Cell Genomics, 2(11), 100177.
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3

Culturing iPSCs with Essential 8 Medium

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iPSCs were cultured in sterile conditions using a supplemented Essential 8™ medium (Gibco #A1517001) and changed every day. At 90% confluency, iPSCs were passaged in Essential 8™ medium with 10 μM Y-27632, a potent inhibitor of ROCK1 (Selleck Chemicals #S1049). Y27632 was withdrawn after 24 h. iPSCs were maintained at 37°C, and 5% CO2.
Zhang H., Jahng J.W., Liu Y., Chase A.J., Perez M.V, & Wu J.C. (2021). Generation of three induced pluripotent stem cell lines (SCVIi014-A, SCVIi015-A, and SCVIi016-A) from patients with LQT1 caused by heterozygous mutations in the KCNQ1 gene. Stem cell research, 55, 102492.
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