Selectra proxl

Blood samples were collected into tubes (containing EDTA) after 12 h overnight fasting at the beginning and the end of the study. The platelet-free plasma was separated by centrifugation and stored at − 80 °C for later biochemical analysis. For participants in the ICR group, a final study visit was scheduled to take place the day after the normal-day diet. Plasma glucose, lipids, lipoproteins, and liver enzymes were analyzed with the colorimetry method via auto-analyzer Selectra ProXL (Vital Scientific, Spankeren, The Netherlands), using commercial kits (Pars Azmoon, Karaj, Iran). Commercially available enzyme-linked immunosorbent assay kits were used to measure plasma insulin (Monobind, Inc., Lake Forest, CA, USA) and adiponectin (Biolegend, San Diego, CA, USA) according to manufacturer’s protocol. Insulin resistance was calculated using the homeostasis model assessment of insulin resistance (HOMA-IR) [19 (link)].

AAT was determined by an immunoturbidimetric assay using a Roche COBAS INTEGRA 400 plus analyzer (Rotkreuz, Switzerland) with 20 mg/dl sensitivity. Red blood cells (RBC), platelets, neutrophil, and lymphocyte counts and their percentages were analyzed automatically by a blood cell counter (Beckman Coulter, Miami, FL, USA). CRP, random blood sugar (BS), SGOT, and SGPT were measured by using the diagnostic kits from Pars Azmoon Company (Pars Azmoon Co., Tehran, Iran) and an autoanalyzer Selectra ProXL (Vital Scientific, Spankeren, The Netherlands). The kits used in this study had a sensitivity of 5 mg/dl for BS, 2 IU/l for SGOT, and 4 IU/l for SGPT. Measurement of ESR was made using the Westergren method and ESR tube according to standard procedure. Troponin concentration was examined by an enzyme-linked immunoabsorbent assay (ELISA) kit (Monobind Inc., Lake Forest, CA, USA). The NLR value was calculated by dividing the neutrophil counts by the lymphocyte counts. O2sat was measured directly by an ABL90 FLEX PLUS blood gas analyzer (Copenhagen, Denmark). Blood samples were taken at admission and during hospitalization. Only the first and last test results of neutrophil and lymphocyte counts, CRP, ESR, and NLR were recorded.

On the last day of the experiment, the feed was cut off at 8:00 p.m. At 8:00 a.m. the next day morning, one pig was selected from each column for blood collection. A total of 10 mL of blood was collected from the veins at the ear margin of the pig, centrifuged at 3500 r/min for 10 min to separate the serum, and sub-packed in sterile centrifuge tubes. Serum samples were received and stored at −80 °C for the determination of serum biochemical parameters and free amino acid contents. During the index analysis, the serum was positioned in an ice water bath to preserve its activity. The contents of the total protein (TP), creatinine (CRE), aspartate aminotransferase (AST), alkaline phosphatase (ALP), alanine aminotransferase (ALT), albumin (ALB), urea (UREA), glucose (GLU), triglyceride (TG), cholesterol (CHO), high-density lipoprotein cholesterol (HDL-C), and low-density lipoprotein cholesterol (LDL-C) (BioSino Bio-Technology & Science Inc., Beijing, China) in serum were measured by an automatic biochemical analyzer (Selectra Pro XL, Vital Scientific, Spankeren, Gelderland, The Netherlands) according to the manufacturer’s instructions. All serum biochemical kits used were purchased from Zhongsheng Beikong Biotechnology Co., Beijing, China.