Pva powder

The PVA six-filament yarn (Mintval^®, Besana In Brianza, Italy) utilized in this investigation was acquired by the melt spinning process of Exceval^® polymer from Kuraray^® (Osaka, Japan). Poly(vinyl alcohol) multifibre/multifilament yarns, exhibiting 41 dtex (36.9 denier; average fiber diameter: 26.10 μm), dissolved in water at 80–90 °C, were utilized in the fabrication of various fibrous structures (Figure 1a), such as biaxial braided ones. PVA powder 99+% (M_w = 89,000–98,000), PVA powder 87–89% (M_w = 85,000–146,000), and PVA powder 99+% (M_w = 146,000–186,000), all hydrolyzed, were acquired through Sigma Aldrich (USA). The molecular weight of hydroxyapatite (HAp, Sigma, USA) is 502.31 g/mol. The remaining reagents were of analytical quality and were employed without any additional refinement. They were acquired from Sigma-Aldrich in St. Louis, MO, USA.

PGS pre-polymers (pPGS), sebacic acid powder (Sigma Aldrich, Seoul, Korea), and glycerol (>99.5%, Sigma Aldrich, Seoul, Korea) were prepared according to the previously published method [58 (link)]. A 1:1.5 molar mixture of sebacic acid and glycerol was placed in a three-neck round bottom flask. The monomers were heated at 120 °C in a nitrogen environment for 24 h. The synthesized solution was filtered using cold acetone (Sigma Aldrich, Seoul, Korea) to remove the loosely cross-linked monomers and the filtrate was used for experiments. The filtrate was reheated and a few drops of N,N-dimethyl formamide (DMF) solvent (Sigma Aldrich, Seoul, Korea) were added to make a homogeneous solution. The PVA solution was prepared by dissolving 1.5 g of PVA powder (Mw~27,000, Sigma Aldrich) in 10 mL of distilled water at 90 °C under continuous stirring for 4 h. Both solutions were blended at various loading ratios of PGS and PVA (2:1.5, 2:1, 2:2 (v/v)) at 50 °C for 30 min. The solution was processed for wet spinning, in which the coagulation bath contained acetone (Sigma Aldrich, Seoul, Korea) and water with an acetone concentration of 70% (v/v). The AuNPs (20 nm in diameter, Sigma Aldrich, Seoul, Korea) were incorporated in a composite conductive microfiber solution.

Buffer A was prepared following a method previously reported in a study by I.ssewanyana et al. Briefly, 1L of phosphate buffer solution was measured and put in a conical flask after which 500μl of tween (Sigma-Aldrich; USA) was dispensed into the PBS to form PBS-tween solution. 5g of PVP powder (Sigma-Aldrich; USA) was weighed using a weighing balance in aluminum foil and added to the above solution after which equal amount of PVA powder was weighed and added to the mixture; proper agitation and mixing was done using a vortexer. 5mls of BSA (Thermofisher Scientific;USA) was pipetted and added to the solution; finally, 0.2g of Sodium azide (Sigma-Aldrich; USA) was weighed under a biosafety hood in an aluminum foil, added to the solution, properly vortexed and mixed. The final solution was labelled with day of preparation and names of those who prepared.