Cytidine 5 triphosphate

2CMC, RBV, guanosine triphosphate (GTP) and cytidine 5’-triphosphate (CTP) were purchased from Sigma-Aldrich and were dissolved in dimethyl sulfoxide (DMSO) (Sigma-Aldrich, St Louis, MO). The HEV-specific antibody was purchased from EMD Millipore (MAB8002).

Cytidine 5′-diphosphate (CDP), cytidine, adenosine 5′-diphosphate (ADP), and 6`-sialyllactose were purchased from GeneChem (Daejeon, Korea). Peptide-N-glycosidase F (PNGase F) and graphitized carbon column were obtained from New England Biolabs (Ipswich, MA, USA) and Alltech (Lexington, MA, USA) respectively. Asialo, galactosylated, biantennary oligosaccharide (G2 glycan), calf intestinal alkaline phosphatase, and biotinylated Sambucus nigra (SNA) lectin were purchased from Prozyme (Hayward, CA), Takara (Tokyo, Japan), and Ey Laboratories (San Mateo, CA, USA) respectively. Solvents for high-performance liquid chromatography (HPLC) including acetonitrile and water were purchased from Burdick and Jackson (Muskegon, MI, USA). 2-Aminobenzoic acid (AA), sodium cyanoborohydride, acetic acid, tetrahydrofuran, triethylamine, trifluoroacetic acid (TFA), CMP-NeuAc, adenosine 5′-triphosphate (ATP), cytidine 5′-triphosphate (CTP), cytidine 5′-monophosphate (CMP), GDP-galactose, bovine β(1,4)-galactosyltransferase, asialofetuin and other reagents (unless stated otherwise) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Lysogeny broth (LB), isopropyl β-d-thiogalactopyranoside (IPTG), NAD⁺, and NADH were
purchased from Research Products International. The protease inhibitor
cocktail, lysozyme, DNase I, UDP-GlcNAc, ManNAc, neuraminic acid,
uridine 5′-diphosphate (UDP), cytidine 5′-triphosphate
(CTP), cytidine 3′,5′-cyclic monophosphate, phosphoenolpyruvate
(PEP), pyruvate kinase, lactate dehydrogenase, sialic acid aldolase,
pyrophosphatase, kanamycin, dithiothreitol (DTT), imidazole, and HEPES
were obtained from Sigma-Aldrich. Ammonium bicarbonate, 2-mercaptoethanol,
KCl, MnCl₂, and MgCl₂ were acquired from Sigma-Aldrich.
Vivaspin 20 spin filters and HisTrap and HiTrap Q HP columns were
obtained from Cytiva. The 10 kDa Nanosep spin filters were purchased
from Pall Corp. (Port Washington, NY). Deuterium oxide was acquired
from Cambridge Isotope Laboratories Inc., and ¹⁸O-labeled
water (98%) was obtained from Medical Isotopes Inc.