Odyssey software 1

Depending on the molecular weight of each protein of interest, a 10-18 % SDS-polyacrylamine gel was used to ensure proper separation of proteins during electrophoresis. Following incubation with the specific primary antibody (see Additional file 4: Table S3) overnight, membranes were incubated for 2 h with either goat anti-mouse IR-800 (Vector Laboratories) or goat anti-rabbit Alexa 680 (Invitrogen) secondary antibodies (1:2500 dilution). Imaging of the labeled membrane was performed on the LI-COR Odyssey system and Odyssey software 1.1 was used to perform densitometry (LI-COR Biotechnology, Lincoln, NE, USA).
Data for H3K18ac were normalized to total H3 and presented as fold change over untreated control. Data for ΔNp63 and STAT6 were normalized to β-tubulin whereas EGFR was normalized to HSP-90 to maintain resolution of the gel at a high molecular weight. All data are presented as fold change over untreated control.

Protein was collected from AECs and airway fibroblasts in culture and electrophoresed on a 12.5% SDS-polyacrylamide gel. Membranes were first incubated overnight with primary antibody (Table 2), then with goat anti-mouse IR-800 (1:2500, Vector Laboratories) or goat anti-rabbit Alexa 680 (1:2500, Invitrogen) secondary antibody, and finally imaged on the LI-COR Odyssey system. Odyssey software 1.1 was used to perform densitometry (LI-COR Biotechnology, Lincoln, NE, USA). Data for AURKA and SMYD3 were normalized to β-tubulin and hsp-90 respectively. A two-tailed unpaired t-test was performed, a p-value of less than 0.05 was considered significant.Table 2

Antibodies used in experiments

Epitope	Host	Company	Catalogue number	Primary antibody dilution
SMYD3	Rabbit	Abcam	ab155018	1/1000
AURKA	Mouse	Cell Signaling	12100	1/500
Hsp90	Mouse	BD Biosciences	610418	1/1000
β-tubulin	Mouse	Millipore	05–661	1/2000
CREBBP	Rabbit	Santa Cruz Biotechnology	sc-369	1/50