Total superoxide dismutase assay kit

PQ was purchased from Sigma Chemical Co. (Sigma-Aldrich, Milan, Italy). ReNcell NSC Maintenance Medium and accutase were obtained commercially from Millipore (Temecula, CA). Epidermal growth factor (EGF) and basic fibroblast growth factor (FGF-2) were purchased from PeproTech. Laminin was purchased from Invitrogen (Carlsbad, CA, USA). Catalase Assay Kit, Malondialdehyde Assay Kit, Lactate Dehydrogenase Assay Kit, BCA Protein Assay Kit, Cell Lysis Buffer for Western and IP, goat anti-rabbit IgG-HRP, and goat anti-mouse IgG-HRP were obtained from Beyotime (Jiangsu, China). Total Superoxide Dismutase Assay Kit was purchased from Dojindo Molecular Technologies (Kumamoto, Japan). Tripure was obtained from Roche (Basel, Switzerland). The AMV first strand cDNA Synthesis Kit was purchased from MBI (Fermentas, Canada). Real-time PCR Kit was obtained from Tiangen Biotech (Beijing, China). Rabbit anti-Nrf2 polyclonal antibody, rabbit anti-Keap1 polyclonal antibody, rabbit anti-PKC polyclonal antibody, and rabbit anti-CKII polyclonal antibody were purchased from GeneTex (San Antonio, USA). Mouse anti-β-tubulin polyclonal antibody was purchased from Boster (Wuhan, China).

The SOD-like activities of the C-dots were tested by using a Total Superoxide Dismutase Assay Kit (S311-10, Dojindo Molecular Technologies) according to the instructions of manufacturer. The SOD-like activity of C-dots with a series of concentrations was represent as the inhibition rate of the competitive WST-1 reaction.