Female C57BL/6J (B6) mice were purchased from Jackson Laboratory (Bar Harbor, ME) at 7 to 8 weeks of age and housed in a biosafety level 2+ (BSL2+) facility at the University of Florida (Gainesville, FL) in accordance with all federal guidelines and as approved by the University of Florida Institutional Animal Care and Use Committee. For i.n. inoculations, B6 mice (Jackson Laboratory) were anesthetized with isoflurane and then inoculated with 104 PFU of virus in 30 μl serum-free DMEM. BALB.IFN-γ−/− mice (Jackson Laboratories) were inoculated with 4 × 105 PFU i.n. For i.p. inoculations, B6 mice were injected with 103 PFU virus in 500 μl serum-free DMEM. Unless otherwise noted, groups of three mice were used per time point, per experiment, for all studies. For blood harvests, blood was collected by heart puncture and placed into BD Microtainers with lithium heparin (catalog number 365965; BD). Buffy coats were separated using lympholyte-M cell separation medium (cI5030; Cedarlane).
Balb ifn γ mice
Manufactured by Jackson ImmunoResearch
BALB.IFN-γ−/− mice are genetically engineered mice with a disruption in the interferon-gamma (IFN-γ) gene. These mice lack the ability to produce IFN-γ, a key cytokine involved in immune responses.
Automatically generated - may contain errors
2 protocols using balb ifn γ mice
1
Intranasal Inoculation of Mice with Virus
2
In vivo Infection of C57BL/6J and BALB.IFN-γ-/- Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female C57BL/6J (B6) mice were purchased from Jackson Laboratory (Bar Harbor, ME) at 7 to 8 weeks of age and housed in a biosafety level 2+ (BSL2+) facility at the University of Florida (Gainesville, FL) in accordance with all federal guidelines and as approved by the University of Florida Institutional Animal Care and Use Committee. For i.n. inoculations, B6 mice (Jackson Laboratory) were anesthetized with isoflurane and then inoculated with 104 PFU of virus in 30 µl serum-free DMEM. BALB.IFN-γ−/− mice (Jackson Laboratories) were inoculated with 4 × 105 PFU i.n. For i.p. inoculations, B6 mice were injected with 103 PFU virus in 500 µl serum-free DMEM. Unless otherwise noted, groups of three mice were used per time point, per experiment, for all studies. For blood harvests, blood was collected by heart puncture and placed into BD Microtainers with lithium heparin (catalog number 365965; BD). Buffy coats were separated using lympholyte-M cell separation medium (cI5030; Cedarlane).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!