All drugs were of the highest purity commercially available. PDBu, GF109203X, L-NAME and phenylephrine were obtained from Sigma Aldrich (St. Louis, MO, USA). Fura-2 was purchased from Molecular Probes (Eugene, OR, USA). Intralipid® 20% was acquired from Fresenius Kabi Korea (Seoul, Korea). Bupivacaine was obtained from Reyon Pharmaceutical Co., Ltd. (Seoul, Korea). Anti-PKC and anti-phospho-PKC (pan) antibodies were purchased from Cell Signaling Technology. Anti-CPI-17 and anti-phospho-CPI-17 (Thr 38) were acquired from Santa Cruz Biotechnology (Santa Cruz, CA, USA). All concentrations were expressed as the final molar concentration. PDBu, GF109203X and Fura-2/AM were dissolved in dimethyl sulfoxide. Unless stated otherwise, all drugs were dissolved in distilled water.
Anti phospho cpi 17 thr 38
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Anti-phospho-CPI-17 (Thr 38) is a lab equipment product that detects the phosphorylation of CPI-17 at threonine 38. It is a specific antibody used for the identification and quantification of the phosphorylated form of this protein.
Automatically generated - may contain errors
Lab products found in correlation
Gf109203x, by Merck Group (1 mentions)
L name, by Merck Group (1 mentions)
Fura 2, by Thermo Fisher Scientific (1 mentions)
Anti pkc, by Cell Signaling Technology (1 mentions)
Anti cpi 17, by Santa Cruz Biotechnology (1 mentions)
Phenylephrine, by Merck Group (1 mentions)
Texas red phalloidin, by Thermo Fisher Scientific (1 mentions)
Prolong mounting solution, by Thermo Fisher Scientific (1 mentions)
Bovine serum albumin (bsa), by Merck Group (1 mentions)
2 protocols using anti phospho cpi 17 thr 38
1
Investigating Vascular Smooth Muscle Contractility
2
Protein Kinase C Regulation Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human thrombin (cell culture grade), fetal bovine serum (FBS), phosphate buffer saline, and bovine serum albumin were purchased from Sigma-Aldrich (St. Louis, MO). Phorbol-12-myristate-13-acetate (PMA), and rottlerin were from EMD/Calbiochem (La Jolla, CA). Anti-PKC (isoform specific), anti-beta-actin and anti-phospho-CPI-17 (Thr38) antibodies were obtained from Santa Cruz Biotech (Santa Cruz, CA); and anti-diphospho-MLC (Thr18/Ser19), anti-total MLC antibodies and anti-phospho-PKD (Ser916) antibodies were from Cell Signaling Technology (Beverly, MA). Anti-mouse and anti-rabbit secondary antibodies conjugated to horse radish peroxidase, enhanced chemiluminescence (ECL), and ECL-Plus were purchased from Amersham Biosciences, Inc /GE Health Sciences (Piscataway, NJ). Texas Red-phalloidin, and Prolong mounting solution were from Molecular Probes (Eugene, OR).
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