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Anti il1r1 pe fab269p

Manufactured by R&D Systems

Anti-IL1R1-PE (FAB269P) is a monoclonal antibody that binds to the Interleukin-1 Receptor Type I (IL-1R1) and is conjugated with the fluorescent dye Phycoerythrin (PE). This product is intended for research use only.

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2 protocols using anti il1r1 pe fab269p

1

Multiparametric Immune Cell Profiling

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Cells were extracellularly stained with the following FITC-conjugated lineage antibodies for sorting as previously described (69 (link)): anti-CD3 (UCHT1), anti-CD1a (HI149), anti-CD14 (HCD14), anti-CD19 (HIB19), anti-TCRαβ (IP26), anti-TCRγδ (B1), anti-CD123 (6H6), anti-CD303/BDCA-2 (201A), anti-FceR1a [AER-37(CRA-1)], anti-CD235a (HI264), and anti-CD66b (G10F5), all from BioLegend. These antibodies were further used within the study: anti-CD3-APC/Cy7 (UCHT1), anti-CD14-APC/Cy7 (HCD14), anti-CD19-APC/Cy7 (HIB19), anti-CD56-PE/Dazzle™ 594 or BV650™ (HCD56), anti-CD34-BV510™/-PE or -FITC (581), anti-NKp44-APC (P44-8), anti-CD73-PE/Dazzle™ 594 (AD2), anti-CD39-APC (A1), anti-Thy1-Alexa Flour® 700 (5E10), and anti-CD117-BV421 and -PE/Cy7 (104D2), all from BioLegend. CD127-PE/Cy5 (R34.34) and NKG2A-PE/Cy7 (Z199) were from Beckman Coulter (California, USA). Anti-IL1R1-PE (FAB269P) was purchased from R&D (Minneapolis, Minnesota). Intranuclear staining of anti-Eomes-PE-eFlour® 610 (WD1928, Invitrogen) and anti- RorγT-PE (clone: AFKJS-9, eBioscience) was performed with the Foxp3 staining kit (Thermo Fischer Scientific) and protocol. The initial gating strategy is shown in Supplementary Figure 1.
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2

Multiparametric Immune Cell Profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were extracellularly stained with the following FITC-conjugated lineage antibodies for sorting as previously described (69 (link)): anti-CD3 (UCHT1), anti-CD1a (HI149), anti-CD14 (HCD14), anti-CD19 (HIB19), anti-TCRαβ (IP26), anti-TCRγδ (B1), anti-CD123 (6H6), anti-CD303/BDCA-2 (201A), anti-FceR1a [AER-37(CRA-1)], anti-CD235a (HI264), and anti-CD66b (G10F5), all from BioLegend. These antibodies were further used within the study: anti-CD3-APC/Cy7 (UCHT1), anti-CD14-APC/Cy7 (HCD14), anti-CD19-APC/Cy7 (HIB19), anti-CD56-PE/Dazzle™ 594 or BV650™ (HCD56), anti-CD34-BV510™/-PE or -FITC (581), anti-NKp44-APC (P44-8), anti-CD73-PE/Dazzle™ 594 (AD2), anti-CD39-APC (A1), anti-Thy1-Alexa Flour® 700 (5E10), and anti-CD117-BV421 and -PE/Cy7 (104D2), all from BioLegend. CD127-PE/Cy5 (R34.34) and NKG2A-PE/Cy7 (Z199) were from Beckman Coulter (California, USA). Anti-IL1R1-PE (FAB269P) was purchased from R&D (Minneapolis, Minnesota). Intranuclear staining of anti-Eomes-PE-eFlour® 610 (WD1928, Invitrogen) and anti- RorγT-PE (clone: AFKJS-9, eBioscience) was performed with the Foxp3 staining kit (Thermo Fischer Scientific) and protocol. The initial gating strategy is shown in Supplementary Figure 1.
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