Li 6400xt open gas exchange system

Manufactured by LI COR

Sourced in United States

The Li-6400xt is an open gas exchange system designed for measuring photosynthesis, respiration, and other gas exchange processes in plants. The system measures the exchange of carbon dioxide and water vapor between a leaf and the surrounding air. It is equipped with a temperature-controlled leaf chamber that provides precise control over environmental conditions. The Li-6400xt collects data on gas exchange, allowing users to analyze plant physiology and function.

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Lab products found in correlation

6 cm2 chamber head, by LI COR (1 mentions) Spad meter, by Konica Minolta (1 mentions)

3 protocols using li 6400xt open gas exchange system

Physiological Responses to Water Treatments

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Physiological responses to water treatments were monitored on each species periodically for 5 months. Leaf-level gas exchange (CO₂ assimilation at ambient C_a, A_max; stomatal conductance of water vapor, g_s; and transpiration rate, E) was measured approximately every 4–5 weeks using an Li-6400xt open gas exchange system (Li-Cor, Inc., Lincoln, NE, USA) on 7–10 randomly selected individuals from each species in each water treatment. Gas exchange measurements were made on the youngest, fully developed leaf (C. mopane and C. apiculatum) or leaflet (A. nigrescens and C. abbreviata) of each plant and cuvette conditions were maintained at [CO₂] = 400 μmol CO₂ mol^-1, relative humidity = 40–60%, and photosynthetically active radiation = 1500 μmol m^-2s^-1 photon flux density. Plants were allowed to stabilize for approximately 2–5 min in the cuvette and a single measurement was recorded. All physiological measurements were made between 10:00 and 14:00 h. Gas exchange calculations were adjusted for leaf area during data processing, if necessary.
Additionally, species growth responses to water treatments were monitored throughout the course of the experiment. Stem height and diameter were measured every 2 weeks on all individuals from each species in both water treatments.

O’Keefe K., Nippert J.B, & Swemmer A.M. (2016). Savanna Tree Seedlings are Physiologically Tolerant to Nighttime Freeze Events. Frontiers in Plant Science, 7, 46.

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Soybean Leaf Gas Exchange Dynamics

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Stomatal conductance (gs), net photosynthetic rate (Pn), transpiration (T), and internal CO2 concentration (Ci) were measured on the second youngest fully-expanded leaf at vegetative, podding and pod-filling stages using an LI-6400XT open gas-exchange system coupled with a 6 cm 2 chamber head (LI-COR Biosciences Inc., Lincoln, NB, USA). The measurements were conducted at photosynthetically active radiation of 1500 µmol photons m -2 s -1 (light-saturated) and CO2 concentrations of 400 µmol mol -1 (ambient) and 800 µmol mol -1 (elevated; podding and pod-filling stages) [9] . The gas exchange measurements were taken on the same day between 10:00 and 15:00 h. The leaf chamber temperature was 28C with 60-70% relative humidity. Chlorophyll concentrations in leaves (lamina) were measured on the same day using a SPAD meter (Minolta, Osaka, Japan) on the same leaf that was used for the gas exchange measurements.

Ly L.T.T. (2020). EVALUATION OF LEAF ‘TISSUE TOLERANCE’ OF Na+ AND Cl– IN SOYBEAN (GLYCINE MAX (L.) MERR.), AND COMMON BEAN (PHASEOLUS VULGARIS L.). International Journal of Agriculture and Environmental Research, 06(06), 853-881.

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Photosynthesis and Chlorophyll Fluorescence

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Photosynthetic responses and Chl fluorescence parameters were measured on fully expanded leaves of the first pair using Li-6400 XT open gas-exchange system (LICOR Biosciences, Lincoln, NE, USA). The system was checked for leaks and calibrated each time before the measurements were taken. Gas exchange and fluorescence measurements at the light-adapted state were done after the 6-h illumination at a 400 ppm CO2 concentration and PPFD of 500 µmol m -2 s -1 (10% blue) according to Pompelli et al. (2010) . The dark-adapted state fluorescence parameters were measured 6 h after the onset of darkness using the same system. Nonphotochemical quenching, proportion of open PSII centres and the maximum quantum efficiency of the PSII photochemistry for both the dark-adapted and lightadapted states were calculated according to Murchie and Lawson (2013) .

Acidri R., Sawai Y., Sugimoto Y., Sasagawa D., Masunaga T., Yamamoto S., & Nishihara E. (2020). Foliar nitrogen supply enhances the recovery of photosynthetic performance of cold-stressed coffee (Coffea arabica L.) seedlings. Photosynthetica, 58(4), 951-960.

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