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4 fluoro 7 nitro 2 1 3 benzoxadiazole nbd f

Manufactured by Dojindo Laboratories
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Sourced in Japan

4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) is a fluorescent labeling reagent. It is commonly used for the fluorescent labeling of amines, amino acids, and peptides.

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Lab products found in correlation

Milli q system, by Merck Group (2 mentions) Mcf 7 cell line, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Hela s3, by American Type Culture Collection (1 mentions) Asf104 serum free medium, by Ajinomoto (1 mentions) Methanol, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Human plasma, by Merck Group (1 mentions) Formic acid, by Fujifilm (1 mentions) Ammonium formate, by Fujifilm (1 mentions) Trifluoroacetic acid, by Fujifilm (1 mentions) Sodium hydroxide (naoh), by Fujifilm (1 mentions) Diethylamine, by Fujifilm (1 mentions) Citric acid, by Fujifilm (1 mentions) Sodium tetraborate, by Fujifilm (1 mentions) Sodium perchlorate monohydrate, by Fujifilm (1 mentions) L alanine, by Fujifilm (1 mentions) L valine, by Fujifilm (1 mentions) L proline, by Fujifilm (1 mentions) Acetonitrile, by Kanto Chemical (1 mentions)

Close spelling variants of this product

NBD-F (6 citations)

3 protocols using 4 fluoro 7 nitro 2 1 3 benzoxadiazole nbd f

1

Fluorescent Labeling of Carcinoma Cells

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Chemicals and cell media were purchased from Wako Pure Chemicals (Osaka, Japan) unless otherwise specified. L-isomer of BPA was from Sigma-Aldrich (St Louis, MO, USA). 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), a fluorescence-derivatization agent, was from Dojindo (Kumamoto, Japan). Pancreatic cancer MIA PaCa−2, cervical cancer HeLa S3, and breast cancer MCF-7 cell lines were from the American Type Culture Collection (Manassas, VA, USA). Pancreatic cancer cell line T3M4 was from Riken Cell Bank (Tsukuba, Japan). Hepatocarcinoma FLC-4 was a gift from Dr. Seishi Nagamori.16 (link) ASF104 serum-free medium was from Ajinomoto (Tokyo, Japan). Fetal bovine serum was from Gibco (Gibco Life Technologies, Grand Island, NY, USA).
Wongthai P., Hagiwara K., Miyoshi Y., Wiriyasermkul P., Wei L., Ohgaki R., Kato I., Hamase K., Nagamori S, & Kanai Y. (2015). Boronophenylalanine, a boron delivery agent for boron neutron capture therapy, is transported by ATB0,+, LAT1 and LAT2. Cancer Science, 106(3), 279-286.
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2

Arginine Metabolism Analysis Protocol

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Arginine (Arg), ornithine (Orn), citrulline (Cit), type-H amino acids standard solution, trifluoroacetic acid (TFA), ammonium formate, and formic acid (FA) were purchased from FUJIFILM Wako Pure Chemical (Osaka, Japan). The fluorescent derivatization agent, 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F), was sourced from Dojindo Laboratories (Kumamoto, Japan). Argininosuccinate (ASA), asparagine, and human plasma were purchased from Sigma-Aldrich (St. Louis, MO, USA). Glutamine was obtained from Kyowa Hakko Kogyo (Tokyo, Japan). Acetonitrile and methanol (HPLC grade) were procured from Merck (Darmstadt, Germany). A Milli-Q system (Merck) was used to purify water. All the other chemicals were of reagent grade.
Sumida Y, & Tsunoda M. (2024). Development of a Two-Dimensional Liquid Chromatographic Method for Analysis of Urea Cycle Amino Acids. Molecules, 29(3), 700.
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3

Derivatization and HPLC Analysis of Amino Acids

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Amino acids (L-serine, L-alanine, L-valine, and L-proline), sodium hydroxide, sodium tetraborate, citric acid, sodium perchlorate monohydrate and diethylamine were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). The 4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) was obtained from Dojindo Laboratories (Kumamoto, Japan). Chloro(dimethyl)octadecylsilane, 95% (ODS-Cl), was obtained from Sigma-Aldrich (St. Louis, MO). Toluene and acetonitrile were of HPLC grade and were obtained from the Kanto Chemical Co., Inc. (Tokyo, Japan). Water was purified using a Milli-Q system (Millipore, Bedford, MA).
Smirnova A., Shimizu H., Mawatari K, & Kitamori T. (2015). Reversed-phase Chromatography in an Extended Nanospace: Separating Amino Acids in Short and Long Nanochannels. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 31(11).
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