Plasma insulin was measured using a commercially available kit (Rat Insulin ELISA kit; Shibayagi, Gunma, Japan) (Rodriguez et al. 2012 (link)). Plasma concentrations of total T4, total T3, and serum concentrations of free T4 were determined using commercial radioimmunoassay (RIA) kits (Coat-A-Count kit; Siemens Healthcare Diagnostics, Los Angeles, CA, USA). Plasma thyroid stimulating hormone (TSH) was determined with a commercially available rat ELISA kit (ALPCO Diagnostics, Salem, NH). Plasma glucose, triglycerides (TG), and nonesterified fatty acids (NEFA) were measured as previously described (Rodriguez et al. 2012 (link)). All samples were analyzed in duplicate with percent coefficients of variability of <10%.
Rat elisa kit
Manufactured by ALPCO
Sourced in United States
The Rat ELISA kit is a laboratory tool used to detect and quantify the presence of specific analytes in rat biological samples. It utilizes the enzyme-linked immunosorbent assay (ELISA) technique, which is a well-established method for measuring the concentration of target molecules in a sample. This kit provides a standardized and reliable way to analyze the levels of the analyte of interest in rat-derived materials.
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Lab products found in correlation
4 protocols using rat elisa kit
1
Plasma Hormone and Metabolite Measurement
2
Insulin and Glycemic Biomarker Evaluation
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Serum insulin was evaluated using a rat ELISA kit (ALPCO Diagnostics). I used the C-peptide enzyme commercial immune assay (Sigma-Aldrich) and HbA1c kits according to the manufacturers’ protocols.
3
Evaluating Metabolic Markers in Rats
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Fasting serum insulin levels were evaluated using a rat ELISA kit (ALPCO Diagnostics, USA). C-peptide (Sigma-Aldrich) and HbA1c (Cusabio Co., China) levels were measured using respective ELISA kits according to the manufacturers’ protocols.
4
Serum Biomarkers Measurement Protocol
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Serum leptin was measured by a rat ELISA kit according to the manufacture’s protocol (Alpco Diagnostics, Salem, NH). The inter-assay coefficient of variation is less than 10% and the lowest detectable level was 10 pg/mL. Serum IGF-1 was measured using an EIA kit as suggested by the vendor (IDS Inc, Fountain Hills, AZ). The inter-assay coefficient of variation was 6% and the lowest detectable level was 2.8 ng/mL. Serum estradiol was measured using an estradiol double antibody RIA according to the vendor’s instructions (Siemens, Plainfield, IN). The inter-assay coefficient of variation was less than 6% and the lowest level detectable is 1.4 pg/ml.
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