Ab10789

Human specific α-synuclein, clone Syn 211 (αSyn) (1:500, Thermo Fisher #AHB0261); anti-phosphorylated α-synuclein at serine 129 [pSyn (S129)] (1:1000, Abcam #ab51253); phosphorylated α-synuclein at tyrosine 125 (pSyn Y125) (1:250, Abcam #ab10789); anti-glial fibrillary acidic protein (GFAP) (1:2000, Abcam #ab4674); anti-glutamate aspartate transporter (GLAST) (1:250, Miltenyi Biotec #130-095-822); anti-ubiquitin (1:500, Thermo Fisher #13-1600); anti-p62/sequestosome 1 (p62) (1:1000, Abcam #ab56416); anti-lysosomal-associated membrane protein 1 (LAMP1) (1:1000, Abcam #ab25245); anti-microtubule associated protein 2 (Map 2) (1:5000, Abcam #ab5392); III β-tubulin (1:1000, Neuromics #MO15013); anti-ionized calcium binding adaptor molecule 1 (Iba1) (1:500, Wako, #019-19,741); β-actin loading control (1:5000, Thermo Fisher #PA1-183); the (E,E)-1-Fluoro-2,5-bis(3-hydroxycarbonyl-4-hydroxy) styrylbenzene (FSB dye) (Congo red derivative) (at 2.5 μM, Santa Cruz #760988-03-2).

Anti-αSyn polyclonal antibody (D119) and monoclonal antibody (Syn204) were obtained from Bioworld Technology Inc. and Cell Signaling Technology, respectively, and were used for western blotting. Anti-pS129-αSyn polyclonal antibody (D1R1R) and anti-β-actin polyclonal antibody (ab8227) were obtained from Cell Signaling Technology and Abcam, respectively, and were used for western blotting. Monoclonal antibody specific for pS129-αSyn (ab51253) and polyclonal antibodies against pY125-αSyn (ab10789), pY133-αSyn (ab194910), and pY136-αSyn (ab194775) used for western blotting and immunohistochemical staining were purchased from Abcam. Casein kinase 2 (CK2, P6010L) was obtained from New England Biolabs Inc. ATP (A2383) was acquired from Sigma-Aldrich.