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Acetyl tubulin 6 11b 1

Manufactured by Santa Cruz Biotechnology
Sourced in United States, France

Acetyl-tubulin 6-11B-1 is a monoclonal antibody that specifically recognizes acetylated tubulin. Acetylation of tubulin is a post-translational modification that occurs on the lysine 40 residue of α-tubulin. This antibody can be used to detect and quantify acetylated tubulin in various cellular and biochemical applications.

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2 protocols using acetyl tubulin 6 11b 1

1

Antibody Profiling for Microtubule Proteins

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Rabbit antibodies against tubulin C102 were a gift of Dr. M. Andreu and HEC1 antibodies were a gift of Dr. PT. Stuckenberg (University of Virginia, Charlottesville, VA, USA). Polyclonal antibodies against TPX2 and PolyE-Tubulin were home-made [32 (link),33 (link)]. All other antibodies are commercially available: Acetyl-tubulin 6-11B-1 (Santa Cruz, Santa Cruz, CA, USA), α-tubulin DM1A (Sigma, Burlington, MA, USA), Vinculin (Sigma), γ-tubulin GTU88 (Sigma), Kinesin 5 (Abcam, Cambridge, UK), pericentrin (Abcam), NEDD1 (Abcam), PRC1 (Santa Cruz), HURP (Proteintech, Chicago, IL, USA), CREST (Europa Bioproducts, Cambridge UK), PLK1 clone 35-206 (Millipore, Burlington, MA, USA), KIF2A (Novus, Littleton, CO, USA), NuMA (Abcam), BUBR1 (Cell Signaling, Danvers, MA, USA), MCAK (Millipore), MYPT1 (Proteintech).
Most chemicals, including Nocodazole, STLC, and MG132, were obtained from Sigma. BI2356 was purchased from MedChemExpress.
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2

Tubulin Antibody Characterization Protocol

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Rabbit antibodies against tubulin C102 was a gift of Dr. M. Andreu (Arevalo et al., 1990) and the antibodies against pE-Tubulin were home-made in rabbit (van Dijk et al., 2007). All other antibodies are commercially available: PAK1T212 (Abcam, Paris, France), α-tubulin YOL1/34 (Santa Cruz Biotechnology, Heidelberg, Germany), α-tubulin DM1A (Sigma, St Quentin Fallavier, France), Acetyl-tubulin 6-11B-1 (Santa Cruz Biotechnology, Heidelberg, Germany), Vinculin (Sigma, St Quentin Fallavier, France), Myc clone 9E10 (Developmental Studies Hybridoma Bank, Iowa City, USA), MEC-17 C6ORF134 (Sigma, St Quentin Fallavier, France), HDAC6 (Santa Cruz Biotechnology, Heidelberg, Germany), PhosphoT423-PAK1 PPAK1 (Cell Signaling, Leiden, The Netherlands). FRAX1036 was a kind gift of Dr. J. Chernoff (Fox Chase Cancer Center, Philadelphia, PA, USA). The other reagents were obtained from the indicated companies: hFibrinogen (Enzyme Research, Swansea, UK), recombinant mouse thrombopoietin (Invitrogen, Thermo Electron SAS, Courtaboeuf, France), IPA-3 (Sigma, St Quentin Fallavier, France), Tubastatin A hydrochloride TBSA (MedChemExpress, Groningen, The Netherlands), Taxol (Sigma, St Quentin Fallavier, France), Trichostatin A TSA (Sigma, St Quentin Fallavier, France).
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