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Dibutylphthalate polystyrene xylene (dpx)

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DPX is a mounting medium used in microscopy applications. It is designed to provide a clear, refractive index-matching mounting solution for prepared samples. DPX facilitates the preservation and protection of specimens while enabling high-quality imaging and analysis.

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Lab products found in correlation

Caffeine, by Merck Group (1 mentions) Eosin y, by Merck Group (1 mentions) Melatonin, by Merck Group (1 mentions) Dimethyl sulfoxide (dmso), by HiMedia (1 mentions) Soybean oil, by Merck Group (1 mentions) Toluidine blue, by Merck Group (1 mentions) Toluidine blue, by HiMedia (1 mentions)

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Xylene (5 citations)

4 protocols using dibutylphthalate polystyrene xylene (dpx)

1

Melatonin, Caffeine, and Soybean Oil Extraction

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Melatonin, caffeine, and soybean oil were procured from Sigma Chemical Co. (St. Louis, MO, USA). DMSO and DPX were procured from Himedia Laboratories Pvt. Ltd. (India). Eosin Y was procured from Merck Ltd., India.
Kushwaha R., Nishad D.K., Bhatnagar A, & Khar R.K. (2021). Melatonin–Caffeine Combination Modulates Gamma Radiation-induced Sperm Malformations in C57BL/6 Male Mice at Sublethal Dose of Gamma Radiation. Journal of Pharmacy & Bioallied Sciences, 13(2), 268-275.
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2

Toluidine Blue Staining of Frozen Sections

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The protocol was adapted from (14 (link)). Briefly, the frozen sections were thawed for 5 minutes at room temperature and then fixed with 4% paraformaldehyde. The slides were then incubated with toluidine blue (Sigma) working solution for 30 seconds at room temperature. Excess dye was drained by quick washes with distilled water. Slides were mounted with DPX (Himedia).
toluidine blue stock= 1g toluidine blue powder dissolved in 100ml of 70% of ethanol (pH 2.3).
Bhattacharjee O., Ayyangar U., Kurbet A.S., Lakshmanan V., Palakodeti D., Ginhoux F, & Raghavan S. (2021). Epithelial-Macrophage Crosstalk Initiates Sterile Inflammation in Embryonic Skin. Frontiers in Immunology, 12, 718005.
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3

Sperm Morphology Analysis via CASMA

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From the sperm solution used for sperm concentration analysis, 10 µL was smeared on a glass slide and allowed to air dry. The dried slides were stained with Sperm Blue® fixative and stain (Microptic SL, Barcelona, Spain) for 3 min, rinsed for 3 s to remove excessive stain, air dried and then mounted (DPX, HiMedia Laboratories Pvt. Ltd., Mumbai, India). Sperm morphology was analysed through computer-aided sperm morphometry analysis (CASMA) using the SCA® (Maree et al., 2010 (link)).
Omolaoye T.S., Skosana B.T, & du Plessis S.S. (2023). The effect of Aspalathin linearis, Cyclopia intermedia and Sutherlandia frutescene on sperm functional parameters of healthy male wistar rats. Frontiers in Physiology, 14, 1211227.
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4

Histological Examination of E. eugeniae

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The histological experiments were carried out to observe the cellular arrangements of E. eugeniae tissues in all the experiments. The respective tissue samples of E. eugeniae were sliced and fixed using 10% formaldehyde (Cat. MB059; Himedia, Mumbai, India) for 24 hours. To remove formaldehyde, the tissues were gently washed with distilled water and dehydration was carried out in 60%-100% of isopropanol (Cat. MB063; Himedia, Mumbai, India) for 1 hour each. After clearing with xylene, the tissues were incubated in paraffin wax (Cat. GRM1137; Himedia, Mumbai, India) for wax impregnation and the 6 μm sections were made using microtome (Cat. MT 1090A; weswax, India). Then, the wax was removed and the tissues were stained with Mayer’s hematoxylin (Cat.MHS1; Sigma-Aldrich, India) and eosin (Himedia, Mumbai, India). The samples were mounted with D.P.X (Himedia, Mumbai, India). The slides were observed under OLYMPUS BX53 upright 3 viewer microscope.
Subramanian E.R., Gopi Daisy N., Sudalaimani D.K., Ramamoorthy K., Balakrishnan S., Selvan Christyraj J.D., Arumugaswami V, & Sivasubramaniam S. (2017). Function of translationally controlled tumor protein (TCTP) in Eudrilus eugeniae regeneration. PLoS ONE, 12(4), e0175319.
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