Plasma TNF-α was measured by the RayBio® Rat TNF- alpha ELISA kit (RayBiotech, Inc., Norcross, Georgia, USA). Standards and samples were pipetted into the wells and TNF-alpha present in a sample was bound to the wells by the immobilized antibody. The wells were washed and biotinylated anti-Rat TNF-alpha antibody was added. After washing away unbound biotinylated antibody, HRP conjugated streptavidin was pipetted to the wells. The wells were again washed, a TMB substrate solution was added to the wells and color developed in proportion to the amount of TNF-alpha bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color was measured at 450 nm.
Raybio rat tnf alpha elisa kit
Manufactured by RayBiotech
Sourced in United States
The RayBio® Rat TNF-alpha ELISA Kit is a quantitative sandwich enzyme immunoassay designed for the measurement of rat tumor necrosis factor-alpha (TNF-alpha) levels in cell culture supernatants, serum, and plasma samples.
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3 protocols using raybio rat tnf alpha elisa kit
1
Rat TNF-alpha ELISA Assay
2
Comprehensive Biomarker Analysis in Rats
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The plasma levels of leptin (Leptin Rat ELISA Kit; Thermo Fisher Scientific, Inc., Waltham, MA, USA), troponin-I (Rat Cardiac Troponin I ELISA Kit (ab246529); Cambridge, MA, USA), FGF-21 (RayBio® Mouse FGF-21 ELISA Kit; Peachtree Corners, GA, USA), IL-6 (RayBio® Rat IL-6 ELISA Kit; Peachtree Corners, GA, USA), and TNF-alpha (RayBio® Rat TNF-alpha ELISA Kit; Peachtree Corners, GA, USA) were measured using commercial ELISA kits, using specific ELISA kits, according to the manufacturer's instructions. Plasma glucose, total cholesterol, HDL, LDL, and triglyceride concentrations were determined using commercially available kits (Olvex Diagnosticum; Saint-Petersburg, Russia).
3
Cytokine Quantification Protocol
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TNF-α levels were measured using the RayBio® Rat TNF-alpha ELISA Kit (RayBiotech, Inc., Norcross, GA) to conform to the instructions on the product label. TGF-β1 was quantitatively checked using the sandwich ELISA (Liu et al., 2020 ), using the PicoKine™ TGF-β1 Mice ELISA Kit (Pleasanton, CA).
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