Vitek2 gp card

Manufactured by bioMérieux

Sourced in France

The VITEK2 GP-card is a compact and efficient laboratory equipment used for the identification and antibiotic susceptibility testing of Gram-positive bacteria. It provides accurate and reliable results to support clinical decision-making.

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Lab products found in correlation

Maldi tof ms, by bioMérieux (2 mentions) Microscan walkaway system, by Siemens (2 mentions) Vitek identification cards, by bioMérieux (2 mentions) Dfp 2000a, by Toshiba (2 mentions) Vitek 2 compact microorganism analysis system, by bioMérieux (2 mentions) Bactec 9050 automatic hemoculture machine, by BD (2 mentions) Co 2 gas incubator, by Sanyo (2 mentions) Vitek 2 gn card, by bioMérieux (2 mentions) Vitek 2 system, by bioMérieux (1 mentions)

8 protocols using vitek2 gp card

Nosocomial Staphylococcus aureus Infections

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The study was conducted in a 1,600-bed university teaching hospital in South India from 2019 to 2021. A total of 133 clinically significant, consecutive, nonrepetitive
Staphylococcus aureusisolated from patients hospitalized for more than 48 hours were included in the study. Repetitive isolates from the same patients and isolates from outpatients were excluded from the study. The study protocol was approved by Institutional Ethics Committee (REF: IEC-NI/19/FEB/68/12).
The source of the isolates was exudates (
n = 105), blood (
n = 18), urine (
n = 7), and respiratory specimens (
n = 3). The significance of the study isolates was ascertained by correlating with Gram stain, significant growth in cultures, and the patient clinical history. The isolates were identified up to species level by standard biochemical tests and automated systems: VITEK2 GP-card (bioMerieux, Marcy l'Etoile, France) and MALDI-TOF MS (bioMerieux, Marcy l'Etoile, France).

Khodabux R.M., Mariappan S, & Sekar U. (2023). Spectrum of Virulence Factors in Clinical Isolates of Staphylococcus aureus and Prevalence of SCCmec Types in Methicillin-Resistant Staphylococcus aureus in a Tertiary Care Center. Journal of Laboratory Physicians, 15(3), 450-461.

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Susceptibility Testing of S. aureus Isolates

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S. aureus isolates were submitted on Dorset transport media (Department of Media Production at NHLS). Organism identification was confirmed using the Vitek 2 GP card (Biomerieux, France). Susceptibility testing was performed on the MicroScan Walkaway system (Siemens Healthcare Diagnostics, USA) using Positive MIC Panel Type 33, with breakpoint ranges from resistant to susceptible. Categorical results and the susceptibility profiles of each antimicrobial agent tested were based on the Clinical Laboratory Standards Institute (CLSI) interpretative criteria [9 ]; Breakpoint tables for interpretation of MICs and zone diameters by EUCAST [10 ] and/or the MicroScan recommendations. The MIC₅₀ and MIC₉₀ (minimum inhibitory concentrations needed to inhibit the growth of 50% and 90% of organisms, respectively) were determined.

Perovic O., Iyaloo S., Kularatne R., Lowman W., Bosman N., Wadula J., Seetharam S., Duse A., Mbelle N., Bamford C., Dawood H., Mahabeer Y., Bhola P., Abrahams S, & Singh-Moodley A. (2015). Prevalence and Trends of Staphylococcus aureus Bacteraemia in Hospitalized Patients in South Africa, 2010 to 2012: Laboratory-Based Surveillance Mapping of Antimicrobial Resistance and Molecular Epidemiology. PLoS ONE, 10(12), e0145429.

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Epidemiology of Staphylococcus haemolyticus

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The study was conducted in a 1,600-bedded, university teaching hospital in South India. A total of 84 clinically significant, consecutive, nonrepetitive
Staphylococcus haemolyticusisolated during the period 2019 to 2021 were included in the study. The study was approved by Institutional Ethics Committee (REF: IEC-NI/19/FEB/68/12)
The source of the isolates was blood (
n = 36), exudative specimens (
n = 38), and urine (
n = 10). The isolates were identified up to species level by standard biochemical tests and automated systems: VITEK2 GP-card (bioMerieux, Marcy l'Etoile, France) and MALDI-TOF MS (bioMerieux, Marcy l'Etoile, France).

Khodabux R.M., Mariappan S, & Sekar U. (2022). Detection of a Novel G2603T Mutation in cfr Harboring Linezolid-Resistant Staphylococcus haemolyticus : First Report from India. Journal of Laboratory Physicians, 15(2), 207-211.

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Pneumonia Pathogen Identification Protocol

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Blood and sputum cultures were routinely performed for patients who were present at the emergency room or were admitted to the hospital due to suspected pneumonia. Simultaneously, patients who had preceding or concurrent flu-like or cold symptoms underwent nasopharyngeal swab sampling to isolate the respiratory viruses. Clinical isolates of S. pneumoniae were identified by conventional biochemical methods and the VITEK 2 GP card (bioMérieux, Marcy l'Etoile, France). Antimicrobial susceptibility was determined using the VITEK 2 system according to the revised Clinical and Laboratory Standards Institute's interpretive criteria for S. pneumoniae.27
Nasopharyngeal swab samples were tested for 12 respiratory virus pathogens, including influenza virus type A and type B, human metapneumovirus (HMPV), respiratory syncytial virus (RSV) type A and type B, rhinovirus, parainfluenza viruses (PIV; types 1, 2, and 3), coronaviruses OC43/229E and NL63, and adenovirus, by using the Seeplex RV assay (Seegene, Inc., Seoul, Korea) based on a multiplex RT-PCR method. Viral DNA and RNA were extracted from each respiratory specimen using the Gene-spin™ kit (iNtRON Biotechnology, Seoul, Korea) according to the manufacturer's instructions and the nucleic acid amplification was conducted using the Seeplex RV master mix as described previously.28 (link)

Yoon Y.K., Yang K.S., Sohn J.W., Lee C.K, & Kim M.J. (2014). Impact of preceding respiratory viral infections on the clinical severity of patients with pneumococcal pneumonia. Influenza and Other Respiratory Viruses, 8(5), 549-556.

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Bacterial Identification Using VITEK 2

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The purity of the isolated suspicious colonies was controlled with Gram staining. Also, oxidase, catalase, beta hemolysis, mannitol, rhamnose, xylose, salicin, dulcite, methyl red, Voges-Proskauer, nitrate reduction and CAMP tests were applied. All colonies added at 3 mL sterile saline solution with sterile swabs in polystyrene test tube to made homogenous 0.5 McFarland turbidity standart suspension using with Vitek 2 DensiCHEK instrument. Then the suspension tube and VITEK 2 GP card (Biomerieux, Marcy l'Etoile, France) placed in the cassette. After that the cassette loaded on Vitek 2 Systems (Biomerieux, Marcy l'Etoile, France) following the manufacturer's guidelines.
The strains, ATCC 15313 for Listeria monocytogenes and ATCC 33090 for Listeria innocua were used as positive controls.

YIGIN A., ALTUN S.K., DEMIRCI M., ESER N., & YOLDAŞ A. (2020). UTILIZATION OF REAL-TIME PCR METHOD FOR IDENTIFICATION OF LISTERIA SPP. FROM HOMEMADE WHITE CHEESE ORIGINATING FROM SOUTHEAST OF TURKEY. Applied Ecology and Environmental Research, 18(4), 5673-5682.

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Microbiological Analysis of Bile and Blood Cultures in PTCD

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A digital fluoroscopy system with a digital subtraction function (DFP-2000A, Toshiba, Japan) was used for conducting PTCD. Further, a VITEK-2 compact microorganism-analysis system (bioMerieux, Marcy l'Etoile, France), a BACTEC-9050 automatic hemoculture machine (Becton Dickinson, Franklin Lakes, NJ), and a CO 2 gas incubator (Sanyo, Japan) were used for bile culture, blood culture, and susceptibility testing. VITEK identification cards (bioMerieux, Marcy l'Etoile, France), including a VITEK-2 GN card for the identification of Gram-negative bacteria and a VITEK-2 GP card for Gram-positive bacteria, were used. The susceptibility cards included AST-GN08 (22007) and AST-GN09 (22008) for Gram-negative bacteria and AST-P534 (22066) and ASTP535 (22067) for Gram-positive bacteria. In addition, a Vitek2YST card was used for the identification of fungi and the AST-YS01 (22108) susceptibility card for fungi.

Wang C., Yu H., He J., Li M., Zhang L., Xu Y., Gao W., Yang X., Guo X, & Guo Z. (2021). Comparative analysis of bile culture and blood culture in patients with malignant biliary obstruction complicated with biliary infection. Journal of cancer research and therapeutics, 17(3).

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Percutaneous Transhepatic Bile Duct Procedure

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PTBD was performed with a Logic-5 ultrasound machine (General Electric, Wauke-sha, WI) and a digital fluoroscopy system with a digital subtraction function (DFP-2000A, Toshiba, Japan). Furthermore, bile culture and susceptibility testing were performed with a CO 2 gas incubator (Sanyo, Japan), VITEK-2 compact microorganism-analysis system (bioMe´rieux, Marcy l'Etoile, France) and a BACTEC-9050 automatic hemoculture machine (Becton-Dickinson, Franklin Lakes, NJ). In addition, VITEK identification cards (bioMe ´rieux, Marcy l'Etoile, France) were applied, including a VITEK-2 GN card for the identification of Gramnegative bacteria and a VITEK-2 GP card for Gram-positive bacteria. The applied susceptibility cards included AST-GN08 (22007) and AST-GN09 (22008) for Gram-negative bacteria and AST-P534 (22066) and AST-P535 (22067) for Gram-positive bacteria, respectively.

Yu H., Yuanyuan S., Guo Z., Xing W., Si T., Guo X, & Liu F. (2018). Multifactorial analysis of biliary infection after percutaneous transhepatic biliary drainage treatment of malignant biliary obstruction. Journal of cancer research and therapeutics, 14(7).

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Phenotypic Characterization of S. aureus

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Phenotypic methods S. aureus isolates were submitted by diagnostic laboratories on Dorset transport medium (Diagnostic Media Products, NHLS, Sandringham). Organism identification was confirmed using the Vitek 2 GP card (bioMerieux, France). Susceptibility testing was performed on the MicroScan Walkaway system (Siemens Healthcare Diagnostics, USA) using the Positive MIC Panel Type 33. Categorical results and susceptibility profiles of most tested antimicrobial agents were based on the Clinical and Laboratory Standards Institute (CLSI) interpretative criteria (7) ; for fosfomycin interpretation of MICs was performed by using EUCAST interpretative criteria 2016 recommendations (8) and mupirocin MICs interpretation was based on manufacturer recommendations. The MIC 50 and MIC 90 (minimum inhibitory concentrations needed to inhibit the growth of 50% and 90% of organisms, respectively) were determined for all tested agents.

Perovic O., Singh-Moodley A., Govender N.P., Kularatne R., Whitelaw A., Chibabhai V., Naicker P., Mbelle N., Lekalakala R., Quan V., Samuel C, & Van Schalkwyk E. (2017). A small proportion of community-associated methicillin-resistant Staphylococcus aureus bacteraemia, compared to healthcare-associated cases, in two South African provinces. European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 36(12).

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