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Hematoxylin eosin stain kit

Manufactured by Vector Laboratories
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The Hematoxylin & Eosin Stain Kit is a laboratory product that provides the necessary components for performing a widely used staining technique. Hematoxylin stains cell nuclei blue, while Eosin stains the cytoplasm and other structures pink or red. This staining method is commonly used in histology and pathology to enhance the contrast and visualization of cellular and tissue structures for microscopic examination.

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Lab products found in correlation

Vectamount mounting medium, by Vector Laboratories (2 mentions) Vs120 microscope, by Olympus (2 mentions) Inkjet plus microscope slides, by Thermo Fisher Scientific (2 mentions) Immpact dab kit, by Vector Laboratories (1 mentions) Biotinylated anti rat igg, by Vector Laboratories (1 mentions)

Spelling variants of this product

Hematoxylin (236 citations) Hematoxylin stain (4 citations) Hematoxylin–eosin (2 citations)

6 protocols using hematoxylin eosin stain kit

1

Histological Analysis of Murine Lung Injury

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The mice (n = 3) were anesthetized with isoflurane; the right lower lung tissue was fixed in 4% paraformaldehyde for 48 hours. After dehydration with graded ethanol, the tissues were embedded in paraffin, cut into 5 μm sections by a microtome, deparaffinized and rehydrated. Hematoxylin & Eosin Stain Kit (Vector Laboratories，California, USA) was used for subsequent staining according to the manufacturer’s protocol. After staining, the samples were observed under a light microscope and photographed at 200× magnification. The degree of lung injury was quantified by the lung injury score [12 (link)].
Zhang J., Zhang Z., Nie X., Liu Y., Qi Y, & Wang J. (2022). Deregulated RNAs involved in sympathetic regulation of sepsis-induced acute lung injury based on whole transcriptome sequencing. BMC Genomics, 23, 836.
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2

Histological Analysis of Murine Hearts

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Unstained, formalin-fixed, paraffin-embedded heart sections (5 μm) of WT and Sorbs2 KO mice were stained with hematoxylin and eosin (H&E) by using Hematoxylin & Eosin stain kit (Vector Laboratories, Inc., Burlingame, CA, USA). H&E stained sections were imaged using an EVOS microscope at the Microscopy and Cell Analysis Core Facility of Mayo Clinic in Rochester campus.
Qian L.L., Sun X., Yang J., Wang X.L., Ackerman M.J., Wang R.X., Xu X., Lee H.C, & Lu T. (2021). Changes in ion channel expression and function associated with cardiac arrhythmogenic remodeling by Sorbs2. Biochimica et biophysica acta. Molecular basis of disease, 1867(12), 166247.
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3

Hematoxylin and Eosin Staining of Brain Sections

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All the products described in this protocol were available in the Hematoxylin & Eosin Stain Kit (Vector Laboratories). Stainings were performed as indicated by the manufacturer. Floating 40–50 μm brain sections were mounted on Inkjet Plus Microscope Slides (Fisher Scientific) and dried until the tissue was completely attached to the surface (1 h approximately). Slides were then rinsed in water for 5 s. Hematoxylin was applied to completely cover the tissue sections and incubated for 5 min. Slides were rinsed in two changes of distilled water (15 s each) to remove excess stain. Bluing reagent was applied to completely cover the tissue and incubated for 15 s. Slides were rinsed in 2 changes of distilled water (15 s each). Slides were dipped in 100% ethanol (10 s). Eosin Y solution was applied completely covering the tissue, incubated for 3 min, and washed in 100% ethanol for 10 s. Finally, the slide was dehydrated in 3 changes of 100% ethanol and mounted in VectaMount Mounting Medium (Vector laboratories). Images were acquired at an Olympus vs-120 microscope using a 20x objective and processed in ImageJ.
Campbell S.C., Muñoz-Ballester C., Chaunsali L., Mills WA I.I.I., Yang J.H., Sontheimer H, & Robel S. (2019). Potassium and glutamate transport is impaired in scar-forming tumor-associated astrocytes. Neurochemistry international, 133, 104628.
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4

Histological Analysis of Ovary and Thyroid

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After 10 % neutral buffered formalin fixed 72 h, ovary and thyroid were separately embedded in paraffin by standard histological method., the tissues were sectioned coronally in 5 μm thickness, and then slices were conducted after dewaxing and hydration procedures. The sections were thereafter routinely stained with Hematoxylin-Eosin Stain kit (Vector laboratories, USA) according to the manufacturer's instructions. The sections of the ovary and thyroid were evaluated under a light microscope and photographed with image acquisition parameters settings at 100 × and 400 × throughout the process.
Eddie-Amadi B.F., Ezejiofor A.N., Orish C.N, & Orisakwe O.E. (2022). Zn and Se abrogate heavy metal mixture induced ovarian and thyroid oxido-inflammatory effects mediated by activation of NRF2-HMOX-1 in female albino rats. Current Research in Toxicology, 4, 100098.
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5

Immunohistological Analysis of Lung Tissue

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Excised lungs were paraffinized and cut into 5 μm sections. Sections were stained with a Hematoxylin & Eosin Stain kit (Vector Laboratories, Burlingame, CA, USA) according to the manufacturer’s protocol. Immunohistology was used to identify the spatial distribution of CD4+ and CD8+ T cells or CD68+ macrophage cells. After deparaffinization and rehydration, antigen retrieval was performed for 20 min at sub-boiling temperature with 10 mM of citrate buffer (pH = 6.0). Immunostaining was performed using VectaStain Elite ABC and Avidin/Biotin Blocking Kits, biotinylated anti-rat IgG, and ImmPACT DAB kit (all from Vector Laboratories), according to the manufacturers’ instructions. The dilutions of the primary antibodies used were as follows: CD68 (DAKO) 1:200, CD4 (Roche, Basel, Switzerland) 1:200, and CD8 (Invitrogen) 1:100. Sections were counterstained with hematoxylin.
Dacheux M.A., Lee S.C., Shin Y., Norman D.D., Lin K.H., E S., Yue J., Benyó Z, & Tigyi G.J. (2022). Prometastatic Effect of ATX Derived from Alveolar Type II Pneumocytes and B16-F10 Melanoma Cells. Cancers, 14(6), 1586.
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6

Hematoxylin and Eosin Staining of Brain Sections

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All the products described in this protocol were available in the Hematoxylin & Eosin Stain Kit (Vector Laboratories). Stainings were performed as indicated by the manufacturer. Floating 40–50 μm brain sections were mounted on Inkjet Plus Microscope Slides (Fisher Scientific) and dried until the tissue was completely attached to the surface (1 h approximately). Slides were then rinsed in water for 5 s. Hematoxylin was applied to completely cover the tissue sections and incubated for 5 min. Slides were rinsed in two changes of distilled water (15 s each) to remove excess stain. Bluing reagent was applied to completely cover the tissue and incubated for 15 s. Slides were rinsed in 2 changes of distilled water (15 s each). Slides were dipped in 100% ethanol (10 s). Eosin Y solution was applied completely covering the tissue, incubated for 3 min, and washed in 100% ethanol for 10 s. Finally, the slide was dehydrated in 3 changes of 100% ethanol and mounted in VectaMount Mounting Medium (Vector laboratories). Images were acquired at an Olympus vs-120 microscope using a 20x objective and processed in ImageJ.
Campbell S.C., Muñoz-Ballester C., Chaunsali L., Mills WA I.I.I., Yang J.H., Sontheimer H, & Robel S. (2019). Potassium and glutamate transport is impaired in scar-forming tumor-associated astrocytes. Neurochemistry international, 133, 104628.
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