Frozen sections were hydrated sequentially from 70% ethanol for 30 min to 95% ethanol for 30 min, then they were placed in 1% Luxol fast blue solution (1 g; S3382; Sigma-Aldrich, St. Louis, MO, USA), 95% ethanol (100 mL), and 10% acetic acid (5 mL) at 60 ° C for 16 h. Slides were rinsed with 95% ethanol, then ddH2O, then differentiated in 0.05% lithium carbonate solution (0.5 g; L4283; Sigma-Aldrich; 1,000 mL ddH2O) for 5 s followed by 70% ethanol for 5s twice, then rinsed with ddH2O. Differentiation steps were repeated until gray matter had cleared. Slides were counterstained with 0.25% cresyl violet solution (0.1 g cresyl violet acetate [C1791, Sigma-Aldrich], 100 mL ddH2O, and 10 drops glacial acetic acid) for 40 s, then rinsed with ddH2O and differentiated in 95% ethanol for 5 min, and then rinsed in 100% ethanol for 5 min twice. Slides were dehydrated from 95% ethanol through 100% ethanol and cleared with xylene; coverslips were mounted with Permount (SP15-500, Fisher Scientific, Pittsburgh, PA, USA).
L4283
Manufactured by Merck Group
Sourced in United States
The L4283 is a laboratory equipment designed for performing various scientific experiments and analyses. It is a versatile and reliable instrument that can be used in a wide range of applications. The core function of the L4283 is to provide accurate and consistent measurements, enabling researchers and scientists to gather data and insights essential for their work. Further details about the intended use or specific applications of this product are not available.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using l4283
1
Luxol Fast Blue and Cresyl Violet Staining
2
Luxol Fast Blue Staining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Frozen sections were hydrated sequentially from 70% ethanol for 30min to 95% ethanol for 30min, then they were placed in 1% Luxol fast blue solution (Luxol fast blue 1g (S3382, Sigma-Aldrich, St. Louis, MO), 95% ethanol 100ml, 10% acetic acid 5ml) at 60° for 16h. Slides were rinsed with 95% ethanol, then ddH2O, then differentiated in 0.05% lithium carbonate solution (lithium carbonate 0.5g (L4283, Sigma-Aldrich), ddH2O 1000ml) for 5s followed by 70% ethanol for 5s twice, then rinsed with ddH2O. Differentiation steps were repeated until gray matter had cleared. Slides were counterstained in 0.25% cresyl echt violet solution (cresyl violet acetate (C1791, Sigma-Aldrich) 0.1gm, ddH2O 100ml, glacial acetic acid 10 drops) for 40s then rinsed in ddH2O and differentiated in 95% ethanol for 5min, then rinsed in 100% ethanol for 5min twice. Slides were dehydrated through from 95% ethanol through 100% ethanol, cleared with xylene and coverslips were mounted with Permount (SP15-500, Fisher Scientific, Pittsburgh, PA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!