DST of NTM was performed using the SLOMYCO Sensititre system (TREK DIAGNOSTIC Systems Ltd., UK). MICs were established by microdilutions in Mueller-Hinton broth in polystyrene 96-well plates containing lyophilized drugs in doubly increasing concentrations (μg/ml): amikacin (AMI) 1,0–64,0; doxycycline (DOX) 2,0–16,0; isoniazid (INH) 0,25–8,0; clarithromycin (CLA) 0,06–64,0; linezolid (LZD) 1,0–64,0; moxifloxacin (MXF) 0,12–8,0; rifampin (RIF) 0,12–8,0; rifabutin (RFB) 0,25–8,0; streptomycin (S) 0,5–64,0; trimethoprim/sulfamethoxazole (SXT) 0,12/2,38–8,0/152,0; ciprofloxacin (CIP) 0,12–32,0; ethambutol (EMB) 0,5–16,0; ethionamide (ETH) 0,3–20,0. Detailed information can be found in the study protocol dx.doi.org/10.17504/protocols.io.nu5dey6 .
MIC values were defined as the lowest concentration of the drug that inhibited the visible growth of the isolates tested. MIC50 and MIC90 values were defined as the drug concentration at which 50% and 90% of the isolates tested showed no visible growth, respectively. The MIC breakpoints of antibiotics displaying resistance were interpreted by CLSI.
MIC values were defined as the lowest concentration of the drug that inhibited the visible growth of the isolates tested. MIC50 and MIC90 values were defined as the drug concentration at which 50% and 90% of the isolates tested showed no visible growth, respectively. The MIC breakpoints of antibiotics displaying resistance were interpreted by CLSI.