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Acetic acid lc ms grade

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Acetic acid (LC-MS grade) is a high-purity solvent used in liquid chromatography-mass spectrometry (LC-MS) applications. It serves as a mobile phase component to facilitate the separation and analysis of analytes. This product meets the stringent quality requirements for use in LC-MS instrumentation.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (3 mentions) Methanol, by Thermo Fisher Scientific (2 mentions) Ammonium acetate, by Thermo Fisher Scientific (2 mentions) Lc ms grade methanol, by Thermo Fisher Scientific (2 mentions) Synergy ultrapure water system, by Merck Group (2 mentions) Sodium l lactate 13c3, by Cambridge Isotopes (2 mentions) Phosphate buffered saline (pbs), by Thermo Fisher Scientific (1 mentions) Amicon ultra 0.5 ml centrifugal filter, by Merck Group (1 mentions) Ammonium acetate, by Merck Group (1 mentions) Ammonium formate, by Merck Group (1 mentions) Ethyl acetate, by Thermo Fisher Scientific (1 mentions) Formic acid, by Thermo Fisher Scientific (1 mentions) 13c12 bpa, by Cambridge Isotopes (1 mentions) Sodium acetate trihydrate, by Carl Roth (1 mentions) Oxylipin, by Cayman Chemical (1 mentions) N hexane, by Carl Roth (1 mentions) Phenylmethylsulfonyl fluoride pmsf, by Merck Group (1 mentions) Sodium hydrogen phosphate, by Carl Roth (1 mentions) Isopropanol lc ms grade, by Thermo Fisher Scientific (1 mentions) Lc ms grade acetonitrile, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Acetic acid (575 citations) LC-MS grade (75 citations) LC-MS-grade acetic acid Optima (2 citations)

12 protocols using acetic acid lc ms grade

1

Quantitative Protein A Purification

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Ultrapure Optima LC–MS-grade
water, LC–MS-grade acetic acid, LC–MS-grade formic acid
and phosphate-buffered saline (PBS) were obtained from Fisher Scientific
(Dublin, Ireland). Ammonium acetate (99.999% trace metal grade), ammonium
formate (≥99.995% trace metal basis) and 4.0 mM l-glutamine
were purchased from Sigma-Aldrich (Wicklow, Ireland). Amicon Ultra-0.5
mL centrifugal filters with a 10 kDa molecular weight cutoff size
and 0.45 and 0.20 μm poly(vinylidene difluoride) (PVDF) membrane
filters were purchased from Merck (Tullagreen, Ireland). BalanCD CHO
Growth A was purchased from FUJIFILM Irvine Scientific (Wicklow Ireland).
Native S. aureus protein A was purchased
from Bio-Rad, (Accuscience, Ireland).
Jakes C., Füssl F., Zaborowska I, & Bones J. (2021). Rapid Analysis of Biotherapeutics Using Protein A Chromatography Coupled to Orbitrap Mass Spectrometry. Analytical Chemistry, 93(40), 13505-13512.
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2

Deuterated BPA Quantification

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Deuterated d6-BPA (dimethyl-d6; 98% isotopic purity and 99% chemical purity; product # 588806-1G) was purchased from Sigma Aldrich (St. Louis, MO) and the corresponding labeled internal standard 13C12-BPA (99%) was obtained from Cambridge Isotope Laboratories, Inc. (Andover, MA). LC/MS grade acetic acid (≥99.7%), acetonitrile (≥99.9%), ammonium acetate (≥99.0%), ethyl acetate (≥99.9%), formic acid (≥ 99.0%), methanol (≥ 99.9%), and water were purchased from Fisher Scientific (Hampton, NH). β-Glucuronidase/arylsulfatase enzyme from Helix pomatia was purchased from Sigma-Aldrich (BGALA-RO, product # 10127698001, Roche Life Science through Sigma Aldrich). All solvents, reagents, and experimental labware were tested for d6-BPA and 13C12-BPA, and none was detected.
Sasso A.F., Pirow R., Andra S.S., Church R., Nachman R.M., Linke S., Kapraun D.F., Schurman S.H., Arora M., Thayer K.A., Bucher J.R, & Birnbaum L.S. (2020). Pharmacokinetics of bisphenol A in humans following dermal administration. Environment international, 144, 106031.
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3

Oxylipin Extraction and Analysis

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Oxylipin and deuterated Oxylipin standards were purchased from Cayman Chemicals (local distributor: Biomol, Hamburg, Germany). LC-MS-grade methanol (MeOH), LC-MS-grade acetonitrile (ACN), LC-MS-grade isopropanol, LC-MS-grade acetic acid were obtained from Fisher Scientific (Schwerte, Germany). Sodium acetate trihydrate, sodium hydrogen phosphate and n-hexane (HPLC grade) were obtained from Carl Roth (Karlsruhe, Germany) and potassium hydroxide (KOH, 85%) from Gruessing GmbH (Filsum, Germany). 2-(1-Thienyl)ethyl 3,4-dihydroxybenzylidenecyanoacetate (2-TEDC) was obtained from Santa Cruz. Ethyl acetate, phenylmethylsulfonyl fluoride (PMSF) and all other chemicals were purchased from Sigma Aldrich (Schnelldorf, Germany).
Rund KM ., Nolte F ., Doricic J ., Greite R ., Schott S ., Lichtinghagen R ., Gueler F , & Schebb NH . (2020). Clinical blood sampling for oxylipin analysis - effect of storage and pneumatic tube transport of blood on free and total oxylipin profile in human plasma and serum. The Analyst, 145(6).
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4

Optimized Sample Preparation for LC-MS

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Methanol, ethanol, water, and formic acid (LC-MS grade) used during the sample preparation procedure were obtained from Sigma-Aldrich (St. Louis, USA). Mobile phase solvents were prepared with LC-MS grade water and Methanol obtained from Merck (Darmstadt, Germany) and LC-MS grade acetic acid from Fisher Scientific (Geel, Belgium).
van Outersterp R.E., Moons S.J., Engelke U.F., Bentlage H., Peters T.M., van Rooij A., Huigen M.C., de Boer S., van der Heeft E., Kluijtmans L.A., van Karnebeek C.D., Wevers R.A., Berden G., Oomens J., Boltje T.J., Coene K.L, & Martens J. (2021). Amadori rearrangement products as potential biomarkers for inborn errors of amino-acid metabolism. Communications Biology, 4, 367.
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5

Solvent Preparation for Analytical Chemistry

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Double-deionized water (Nanopure®, Werner GmbH, Leverkusen, Germany) was used. Ethanol (HPLC grade), methanol (HPLC grade), n-hexane (HPLC grade) and acetic acid (LC-MS grade) were purchased from Fisher Scientific (Loughborough, UK). Acetonitrile (HPLC and LC-MS grade) were obtained from Honeywell Speciality Chemicals (Seelze, Germany). Acetic acid (HPLC grade) was purchased from Applichem (Darmstadt, Germany). Ethyl acetate (analytical grade) was purchased from Carl Roth (Karlsruhe, Germany). For TLC, spray reagent anisaldehyde (>98%) from Merck (Darmstadt, Germany) and chloroform (HPLC grade) from VWR Int. S.A.S (Darmstadt, Germany) were used. Dimethyl sulfoxide-d6 (99.9% D) from Deutero GmbH (Kastellaun, Germany) and tetramethylsilane (TMS) from Sigma-Aldrich (Deisenhofen, Germany) were used for NMR spectroscopic measurements.
Kiene M., Blum S., Jerz G, & Winterhalter P. (2023). A Comparison between High-Performance Countercurrent Chromatography and Fast-Centrifugal Partition Chromatography for a One-Step Isolation of Flavonoids from Peanut Hulls Supported by a Conductor like Screening Model for Real Solvents. Molecules, 28(13), 5111.
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6

Reliable Steroid Profiling via Mass Spectrometry

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E2 and Te were from Sigma-Aldrich (Saint-Louis, MO, USA). Estriol, estrone, and deuterated internal standards, 17-β estradiol (E2-D5) and testosterone (Te-D3) were from Cerilliant (Round Rock, TX, USA). All compounds had a purity greater than 99%. 17-OH-progesterone, aldosterone, androstenedione, corticosterone, cortisol, cortisone, 11-deoxycortisol, dehydroepiandrosterone, dehydroepiandrosterone sulfate, dihydrotestosterone, and progesterone were from Chromsystems Steroid Panel I & II kits (Grafelfing, Germany). Water, methanol, acetonitrile, 2-propanol, acetone, and acetic acid (LCMS grade) were from Fisher Scientific (Fair Lawn, NJ, USA). Calibrators and quality controls were from Chromsystems Steroid Panel II kit previously mentioned. Ultra-low steroid human serum (DC Mass Spect Gold) was from Golden West Biologicals, Inc. (Temecula, CA, USA) and is certified to contain < 0.03 pg/mL and < 0.025 ng/dL E2 and Te, respectively. Serum separator and clot activator blood collection tubes were obtained from Beckton Dickenson (Franklin Lakes, NJ, USA).
Schofield R.C., Mendu D.R., Ramanathan L.V., Pessin M.S, & Carlow D.C. (2017). Sensitive Simultaneous Quantitation of Testosterone and Estradiol in Serum by LC-MS/MS without Derivatization and Comparison with the CDC HoSt Program. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 1048, 70-76.
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7

Metabolite Standards for Metabolomics

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Acetonitrile (LC–MS grade) and methanol (LC–MS grade) were purchased from Sigma-Aldrich (St. Louis, MO). Ammonium acetate (LC–MS grade) and acetic acid (LC–MS grade) were obtained from Fisher Scientific (Pittsburgh, PA). DI water was provided in-house by a Synergy Ultrapure Water System from EMD Millipore (Billerica, MA). The metabolite standards in the Northwest Metabolomics Research Center (NW-MRC) internal database (included in Table S1) were purchased from Sigma-Aldrich or Fisher Scientific. Stable isotope labeled amino acids were purchased from Cambridge Isotope Laboratories (Tewksbury, MA).
Gu H., Zhang P., Zhu J, & Raftery D. (2015). Globally Optimized Targeted Mass Spectrometry: Reliable Metabolomics Analysis with Broad Coverage. Analytical chemistry, 87(24), 12355-12362.
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8

NMR Metabolite Profiling: Reagents and Standards

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Acetonitrile (ACN), ammonium acetate, and acetic acid (LC-MS grade) were purchased from Fisher Scientific (Pittsburgh, PA). Sodium phosphate, monobasic (NaH2PO4), Sodium phosphate, dibasic (Na2HPO4), and 3-(trimethylsilyl)propionic acid-2,2,3,3-d4 sodium salt (TSP) were obtained from Sigma-Aldrich (St. Louis, MO). The standard compounds corresponding to the measured metabolites were purchased from Sigma-Aldrich and Fisher Scientific. D2O, stable isotope-labeled tyrosine and lactate internal standards (L-tyrosine-13C2 and sodium-L-lactate-13C3) were purchased from Cambridge Isotope Laboratories, Inc. (Tewksbury, MA). The purities of non-labeled standards were >95–99%, whereas the purities of the two 13C-labeled compounds were > 99%.
Lohavanichbutr P., Zhang Y., Wang P., Gu H., Nagana Gowda G.A., Djukovic D., Buas M.F., Raftery D, & Chen C. (2018). Salivary metabolite profiling distinguishes patients with oral cavity squamous cell carcinoma from normal controls. PLoS ONE, 13(9), e0204249.
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9

Preparation and Purification of Stilbenoid Standards

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Double-deionized water (Nanopure®, Werner GmbH, Leverkusen, Germany) was used. Ethanol (HPLC grade) and acetic acid (LC-MS grade) were purchased from Fisher Scientific (Loughborough, UK). Acetonitrile (HPLC and LC-MS grade) was obtained from Honeywell Specialty Chemicals (Seelze, Germany). Formic acid (HPLC grade) and lactic acid (90%) were purchased from VWR Int. S.A.S (Darmstadt, Germany). For NADES preparations, betaine (98%) and urea (99.5%) were purchased from Fisher Scientific (Loughborough, UK). D-Fructose (99%) and D-glucose (99%) were obtained from Carl Roth (Karlsruhe, Germany). Amberlite®XAD-16N, choline chloride (>98%), and 1,2-propanediol (99%) were purchased from Sigma-Aldrich (Deisenhofen, Germany). Trans-resveratrol standard (>98%) was obtained from Carl Roth (Karlsruhe, Germany), and trans-ε-viniferin (>94%, λ 280 nm, in-house method, [35 (link)]) was isolated from the commercial product Vineatrol®30 (Breko GmbH, Bremen, Germany). Stilbenoid tetramers r-2-viniferin (95%, λ 280 nm) and r-viniferin (91%, λ 280 nm) were isolated from de-colored Vitisin® Powder (Actichem, Montauban, France) using an in-house method [46 ]. Each sample for UHPLC and LC-MS analysis was filtered through 0.2 µm PTFE syringe filters from Agilent Technologies (Waldbronn, Germany).
Kiene M., Zaremba M., Januschewski E., Juadjur A., Jerz G, & Winterhalter P. (2024). Sustainable In Silico-Supported Ultrasonic-Assisted Extraction of Oligomeric Stilbenoids from Grapevine Roots Using Natural Deep Eutectic Solvents (NADES) and Stability Study of Potential Ready-to-Use Extracts. Foods, 13(2), 324.
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10

RNA Purification and Enzymatic Modification

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RNase T1 and bacterial alkaline phosphatase (BAP) were purchased from Worthington Biochemical (Lakewood, NJ). S. cerevisiae tRNAPhe and ammonium acetate (LC-MS grade) were purchased from Sigma Aldrich. Acetic acid (LC-MS grade) and methanol (LC-MS grade) were purchased from Fisher Scientific. High purity (18 MOhm) water was generated in-house with a Barnstead Nanopure System (Thermo Scientific).
Lobue P.A., Yu N., Jora M., Abernathy S, & Limbach P.A. (2018). Improved Application of RNAModMapper – an RNA Modification Mapping Software Tool – for Analysis of Liquid Chromatography Tandem Mass Spectrometry (LC-MS/MS) Data. Methods (San Diego, Calif.), 156, 128-138.
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