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The RB-373 is a lab equipment product manufactured by Thermo Fisher Scientific. It is designed for use in laboratory settings, but a detailed description of its core function cannot be provided while maintaining an unbiased and factual approach. Additional information about the intended use or features of this product is not available.

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Lab products found in correlation

K4003, by Agilent Technologies (1 mentions) P0450, by Agilent Technologies (1 mentions) Ab5690, by Abcam (1 mentions) Oct compound, by Sakura Finetek (1 mentions) Protein block serum free, by Agilent Technologies (1 mentions) Mayer s hematoxylin solution, by Merck Group (1 mentions) Apoptag peroxidase in situ apoptosis detection kit, by Merck Group (1 mentions)

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RB-373-A (2 citations)

2 protocols using rb 373

1

Immunohistochemical Staining of Lung Tissue

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The extirpated left lung was inflated to 20 cm H2O with OCT compound (Sakura Finetek Japan, Tokyo, Japan) and frozen in isopentane cooled in liquid nitrogen. Sections of 10 μm were fixed with cold acetone and blocked with Protein Block Serum-Free® (Dako, Glostrup, Denmark). They were then incubated with the first antibodies: rabbit antimyeloperoxidase antibodies (1:200, RB-373; Thermo Fisher Scientific, Waltham, Massachusetts) for neutrophil staining, rat antimouse Mac-3 antibodies (1:500; BioLegend, San Diego, California) for macrophage staining, rabbit anti-CD3 polyclonal antibodies (1:100; ab5690, Abcam, Cambridgeshire, UK) and rat anti-CD19 monoclonal antibodies (1:500; ab25232, Abcam, Cambridgeshire, UK) for lymphocyte staining. The EnVision® + system–HRP labeled polymer antirabbit (K4003; DAKO, Glostrup, Denmark) and polyclonal rabbit antirat immunoglobulins (P0450; DAKO, Glostrup, Denmark) were used as the second antibodies. Diaminobenzidine (DAKO, Glostrup, Denmark) was used to visualize the sections, and haematoxylin was used for counterstaining.
Iwata T., Ito I., Niimi A., Ikegami K., Marumo S., Tanabe N., Nakaji H., Kanemitsu Y., Matsumoto H., Kamei J., Setou M, & Mishima M. (2015). Mechanical Stimulation by Postnasal Drip Evokes Cough. PLoS ONE, 10(11), e0141823.
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2

Histological Assessment of Liver Injury

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Livers were sliced in 5-μm sections and were stained for hematoxylin and eosin (H&E). H&E staining was used to score the grade of liver inflammation while liver fibrosis was assessed with picrosirius red (PSR) staining. For PSR staining, liver sections were incubated for 2 hours at room temperature with an aqueous solution of saturated picric acid containing 0.1% Direct Red (MilliporeSigma). To study liver cell death, terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay was performed using manufacturer’s instructions (ApopTag Peroxidase In Situ Apoptosis Detection Kit, MilliporeSigma). Immunohistochemistry (IHC) staining for myeloperoxidase (MPO) (1:200, Thermo Fisher Scientific, catalog RB-373) was performed on formalin-fixed, paraffin-embedded livers according to manufacturer’s instruction and counterstained with Mayer’s Hematoxylin solution (Sigma-Aldrich).
Calcagno D., Chu A., Gaul S., Taghdiri N., Toomu A., Leszczynska A., Kaufmann B., Papouchado B., Wree A., Geisler L., Hoffman H.M., Feldstein A.E, & King K.R. (2022). Nlrp3 activation causes spontaneous inflammation and fibrosis that mimics human NASH. Hepatology (Baltimore, Md.), 76(3), 727-741.
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