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Naphthalene 2 3 dicarboxaldehyde nda

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Naphthalene-2,3-dicarboxaldehyde (NDA) is a chemical compound used as a fluorescent labeling agent in analytical chemistry. It is commonly used in the detection and quantification of various analytes, such as amino acids and amines, through derivatization reactions. NDA-labeled compounds can be analyzed using techniques like high-performance liquid chromatography (HPLC) and capillary electrophoresis.

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Lab products found in correlation

Acetonitrile, by Thermo Fisher Scientific (2 mentions) Sodium hydroxide (naoh), by Thermo Fisher Scientific (2 mentions) Sodium cyanide nacn, by Merck Group (2 mentions) Dihydroethidium dhe 2 7 dichlorofluorescin diacetate dcfh da, by Thermo Fisher Scientific (2 mentions) Fluorescein isothiocyanate fitc dextran, by Merck Group (1 mentions) Sybr green pcr master mix, by Roche (1 mentions) 2 7 dichlorofluorescin diacetate dcfh da, by Merck Group (1 mentions) Dihydroethidium dhe, by Merck Group (1 mentions) Milli q filtration system, by Merck Group (1 mentions) Taurine tau, by Merck Group (1 mentions) Hplc grade dimethylsulfoxide, by Thermo Fisher Scientific (1 mentions) Ph meter, by Thermo Fisher Scientific (1 mentions) Sodium tetraborate, by Merck Group (1 mentions) Boric acid, by Thermo Fisher Scientific (1 mentions) Ethanol, by Thermo Fisher Scientific (1 mentions) Lipopolysaccharides (lps), by Merck Group (1 mentions) Anhydrous dimethyl sulfoxide dmso, by Merck Group (1 mentions) 25 ml polystyrene culture flasks, by Thermo Fisher Scientific (1 mentions) Milli q synthesis a10, by Merck Group (1 mentions) L carnosine, by Merck Group (1 mentions)

Close spelling variants of this product

Naphthalene-2,3-dicarboxaldehyde Naphthalene

10 protocols using naphthalene 2 3 dicarboxaldehyde nda

1

Fluorescence-Based Cellular Assays

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Dichlorodihydrofluorescein diacetate (DCFH-DA), dihydroethidium (DHE), lipopolysaccharide (LPS), and fluorescein-isothiocyanate- (FITC-) dextran (average MW 3,000-5,000) were purchased from Sigma-Aldrich (California, USA). Naphthalene-2,3-dicarboxal-dehyde (NDA) was obtained from Life Technologies (Carlsbad, CA, USA). SYBR Green PCR Master Mix was purchased from Roche (Basel, Switzerland). All other reagents used were of analytical grade.
Su H., Mo J., Ni J., Ke H., Bao T., Xie J., Xu Y., Xie L, & Chen W. (2020). Andrographolide Exerts Antihyperglycemic Effect through Strengthening Intestinal Barrier Function and Increasing Microbial Composition of Akkermansia muciniphila. Oxidative Medicine and Cellular Longevity, 2020, 6538930.
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2

Antioxidant Activity of Chinese Pecans

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Chinese pecans were obtained from Lin'an Tongda Food Co., Ltd. (Hangzhou, China), which were harvested in late September 2021. And the CM was obtained from the local farm market of Hangzhou, after Chinese pecans being oil extraction. 2′,7′-dichlorofluorescin diacetate (DCFH-DA), and dihydroethidium (DHE) were obtained from Sigma-Aldrich (St. Louis, MO, U.S.A.). 5,5‘,6,6’-Tetrachloro-1,1‘,3,3’-tetraethylbenzimidazolylcarbocyanine iodide (JC-1) was obtained from Yeasen (Shanghai, China). Naphthalene-2,3-di-carboxal-dehyde (NDA) was obtained from Life Technologies. 3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2Htetrazolium bromide (MTT), methyl viologen dichloride were purchased from Aladdin (Shanghai, China). All other reagents were of analytical grade.
Yan F., Wang Q., Teng J., Wu F, & He Z. (2022). Preparation process optimization and evaluation of bioactive peptides from Carya cathayensis Sarg meal. Current Research in Food Science, 6, 100408.
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3

Non-Aqueous Capillary Electrophoresis Reagents

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Chemicals were purchased from Sigma Aldrich (St. Louis, MO) unless otherwise indicated. Ultrapure deionized (DI) water was prepared using a Milli-Q filtration system (Millipore, Belford, MA). Phosphate-buffered saline (PBS) was made by dissolving 154 mM NaCl in 20 mM phosphate buffer (pH 7.4). Pyrophosphate buffer was made by mixing appropriate amounts of monobasic and dibasic pyrophosphate solutions to yield 44 mM pyrophosphate (pH 8.0). Acidified acetone was made by mixing acetone, water, and HCl (12 M) in a volume ratio of 40:6:1. Amino acids were stored as solutions dissolved in PBS except L-norleucine (L-Nle), which was in 100 mM borate (pH 9.5) buffer. Naphthalene-2,3-dicarboxaldehyde (NDA) and potassium cyanide (KCN) were purchased from Invitrogen (Grand Island, NY). For non-aqueous capillary electrophoresis (NACE) separations, formamide (FA), N-methyl formamide (NMF), hydroxypropyl γ-cyclodextrin (HP-γ-CD), (1S)-(+)-camphorsulfonic acid (CS), quinine (QN) and sodium acetate were used. For D-Ala peak identification, flavin adenine dinucleotide (FAD), DAAO from porcine kidney (8.2 U/mg solid), and catalase from bovine liver (10000–40000 U/mg protein, 34 mg protein/mL suspension) were dissolved in PBS. These solutions contained 5 mM FAD, 15 U/mL DAAO and 15.6–62.5 U/mL catalase, respectively. Dichloromethane was used for liquid-liquid extraction.
Ota N., Rubakhin S.S, & Sweedler J.V. (2014). D-Alanine in the Islets of Langerhans of Rat Pancreas. Biochemical and biophysical research communications, 447(2), 328-333.
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4

Amino Acid Quantification Protocol

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Arginine (Arg), aspartate (Asp), citrulline (Cit), glutamate (Glu), histamine (Hist), and taurine (Tau) were obtained from Sigma Aldrich (St Louis, MO). Standards of each amine were prepared at 2 mM concentrations in 18.2 MΩ /cm deionized water (Millipore, Billerica, MA). Subsequent dilutions of each stock solution were made prior to analysis. Naphthalene-2,3-dicarboxaldehyde (NDA) (Invitrogen, Carlsbad, CA) was prepared in acetonitrile (Fisher Scientific, Pittsburgh, PA) to a concentration of 5 mM. Sodium cyanide (NaCN) (Sigma Aldrich) was dissolved in water to a final concentration of 10 mM. Stock solutions of both NDA and NaCN were made weekly and stored at 4°C, protected from light exposure. Stock solutions of sulfobutylether-β-cyclodextrin (SBEC) (Life Technologies, Grand Island NY) were made on a weekly basis to a concentration of 10 mM in deionized water and stored at 4°C. Finally, the background electrolyte (BGE) consisted of 1.4 mM SBEC, 10% by volume HPLC-grade dimethylsulfoxide (Fisher Scientific), and sodium tetraborate (Sigma Aldrich) at a final concentration of 15 mM. Finally, the pH of the BGE was measured using a pH meter and adjusted to 9.2 with 1 M sodium hydroxide (Fisher Scientific).
OBORNY N.J., COSTA E.E., SUNTORNSUK L., ABREU F.C, & LUNTE S.M. (2016). Evaluation of a Portable Microchip Electrophoresis Fluorescence Detection System for the Analysis of Amino Acid Neurotransmitters in Brain Dialysis Samples. Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 32(1), 35-40.
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5

Sensitive CE-LIF Assay for D-Aspartate

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Chemicals were purchased from Sigma-Aldrich (St. Louis, MO) unless stated otherwise. Aqueous solutions were prepared with ultrapure deionized (DI) water from an ELGA Purelab Ultra water system (USFilter, Lowell, MA). Phosphate buffer saline (PBS) was purchased from Mediatech (Manassas, VA). Amino acids were stored as neat aqueous solutions at 14 °C. Naphthalene-2,3-dicarboxaldehyde (NDA) was purchased from Invitrogen (Carlsbad, CA). For the CE separations, 2-(N-morpholino)ethanesulfonic acid (MES), potassium bromide (KBr), and quaternary ammonium β-cyclodextrin (QAβCD) (CTD Holdings, Alachua, FL) were used. See the Supporting Information for experimental details relating to D-aspartate oxidase (D-AspO) cloning and purification. For D-Asp peak confirmation by enzymatic degradation via D-AspO, flavin adenine dinucleotide (FAD) and catalase (10,000–40,000 U/mg protein, 34 mg protein/mL suspension) from bovine liver were prepared in PBS. Homemade desalting tips were loaded with polymer slugs punched out from Empore polystyrene-divinylbenzene solid phase extraction (SPE) disks from 3M (St. Paul, MN).
Patel A.V., Kawai T., Wang L., Rubakhin S.S, & Sweedler J.V. (2017). Chiral Measurement of Aspartate and Glutamate in Single Neurons by Large-volume Sample Stacking Capillary Electrophoresis. Analytical chemistry, 89(22), 12375-12382.
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6

Murine RAW 264.7 Cell Culture Protocol

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Murine RAW 264.7 cells (ATCC® TIB71™), Dulbecco’s Modified Eagle’s Medium (DMEM), phenol red-free DMEM, fetal bovine serum (FBS), and penicillin/streptomycin antibiotic solution were purchased from American Type Culture Collection (ATCC, Manassas, VA, USA). L-carnosine, sodium cyanide (NaCN), anhydrous dimethyl sulfoxide (DMSO), phosphate-buffered saline (PBS), Trypan blue solution, lipopolysaccharides (LPS), Triton X-100, and bovine serum albumin (BSA) were all supplied by Sigma-Aldrich (St. Louis, MO, USA). Sodium hydroxide (NaOH), hydrochloric acid (HCl), 25 mL polystyrene culture flasks, boric acid, and ethanol (95%) were obtained from Fisher Scientific (Pittsburgh, PA, USA). Interferon-γ (IFN-γ) was supplied by Calbiochem (Gibbstown, NJ, USA). Naphthalene-2,3-dicarboxaldehyde (NDA) was obtained from Invitrogen (Carlsbad, CA, USA). Polyethersulfone (PES) membrane (3K) was purchased from VWR International (West Chester, PA, USA). C-Chip disposable hemocytometer was purchased from Bulldog Bio, Inc. (Portsmouth, NH, USA). All water used was Ultrapure (18.3 MΩ cm) (Milli-Q Synthesis A10, Millipore, Burlington, MA, USA).
Fresta C.G., Hogard M.L., Caruso G., Melo Costa E.E., Lazzarino G, & Lunte S.M. (2016). Monitoring carnosine uptake by RAW 264.7 macrophage cells using microchip electrophoresis with fluorescence detection. Analytical methods : advancing methods and applications, 9(3), 402-408.
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7

HPLC Analysis of Neurotransmitters

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Monobasic sodium phosphate, disodium ethylenediamine tetraacetate (Na2EDTA), 85% o-phosphoric acid, acetonitrile, methanol, hydrochloric acid (HCl), sodium hydroxide (NaOH), and 0.3 μm alumina powder were obtained from Fisher Scientific (Pittsburgh, PA). Ammonium acetate, 1-octanesulfonic acid [sodium salt] (SOS), sodium tetraborate decahydrate, boric acid, lithium tetraborate, lithium dodecyl sulfate (LDS), tetradecyltrimethylammonium bromide (TTAB), β-alanine (β-Ala), sodium cyanide, 4-hydroxybenzoic acid (4-HBA), L-glutamic acid (Glu), L-aspartic acid (Asp), L-arginine (Arg), γ-amino-n-butyric acid (GABA), DL-2-aminoadipic acid (AAP), sodium cyanide, 3,4-dihydroxybenzylamine (DHBA), 3,4-dihydroxyphenethylamine hydrochloride (DA), L-arterenol (NE), homovanillic acid (HVA), 3,4-dihydroxyphenylacetic acid (DOPAC), 5-hydroxytryptamine (5-HT), 5-hydroxyindoleacetic acid (5-HIAA) were obtained from Sigma-Aldrich (St. Louis, MO). Naphthalene-2,3-dicarboxaldehyde (NDA) was obtained from Invitrogen (Carlsbad, CA). All solutions were prepared in 18.2 MΩ distilled, deionized water (Labconco, Kansas City, MO) and filtered through 0.22 μm pore size membrane filters prior to use unless otherwise noted.
Crick E.W., Osorio I., Frei M., Mayer A.P, & Lunte C.E. (2014). Correlation of 3-Mercaptopropionic Acid Induced Seizures and Changes in Striatal Neurotransmitters Monitored by Microdialysis. European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 57, 25-33.
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8

Evaluating Antibiotic Effects on C. elegans

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For the C. elegans assay, the detailed method is provided in the methods section of the supplemental material. In all, four to six synchronized L2 larvae were transferred to agar plates containing 10× the MIC of each antibacterial agent. Under this antimicrobial load, the food for the worms (E. coli bacterium cells) will have died (but do not lyse) during the first day of the experiment and, by the end of the experiment, the worms would have run out of food, thus the offspring would not mature. This did not affect our observations over the timescale of our experiment. Four results were recorded for 5 days: (i) number of live worms, (ii) motility (head swing and body-bending frequency per minute), (iii) growth (body length and body width), and (iv) regeneration (generation of offspring, size, number, and motility of them were considered) rates.
Multiple fluorescent sensor probes were employed, including dihydroethidium (DHE), 2′,7′dichlorofluorescin diacetate (DCFH-DA), and naphthalene-2,3-dicarboxal-dehyde (NDA), all obtained from the Invitrogen to detect the O2.−, ROS, and glutathione, respectively.
Pormohammad A., Firrincieli A., Salazar-Alemán D.A., Mohammadi M., Hansen D., Cappelletti M., Zannoni D., Zarei M, & Turner R.J. (2023). Insights into the Synergistic Antibacterial Activity of Silver Nitrate with Potassium Tellurite against Pseudomonas aeruginosa. Microbiology Spectrum, 11(4), e00628-23.
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9

Quantitative Analysis of Amino Acids by CE-LIF

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Aqueous solutions prepared in either LC-MS grade water or ultrapure water (Milli-Q Direct Water Purification System, MilliporeSigma, Burlington, MA, USA) were used for CE-LIF measurement. For CE-LIF detection, d/l-Ser and d/l-Asp were derivatized by reaction with naphthalene-2,3-dicarboxaldehyde (NDA) (Invitrogen, Carlsbad, CA, USA). A 4 μL mixture of an aliquot of the islet extract, 20 mM potassium cyanide (KCN) in 100 mM borate buffer, and 20 mM NDA in acetonitrile (ACN) was prepared in a 1:2:1 volume ratio. The mixture was allowed to react for 2 min in the dark at room temperature and then diluted to 10 μL of total volume by water. The samples were further desalted using a procedure similar to one previously used by our group [28 (link)] (see the Supplementary Materials). For quantitation using linear calibration curves, d/l-Ser or d/l-Asp standards of different concentrations ranging from 0.025–100 μM for d and 0.125–500 μM for l were prepared, NDA-derivatized as previously described, and diluted to 100 μL by water for CE-LIF analysis.
Lee C.J., Schnieders J.H., Rubakhin S.S., Patel A.V., Liu C., Naji A, & Sweedler J.V. (2022). d-Amino Acids and Classical Neurotransmitters in Healthy and Type 2 Diabetes-Affected Human Pancreatic Islets of Langerhans. Metabolites, 12(9), 799.
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10

Oxidative Stress and Antioxidant Levels in Nematodes

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ROS levels lead to oxidative stress, inflammation, and cellular disruption, while reduced glutathione (GSH) is a cellular antioxidant against oxidative stress, and it is essential to cellular protection [104 (link)]. Multiple fluorescent sensor probes were employed including dihydroethidium (DHE), 2′,7′-dichlorofluorescin diacetate (DCFH-DA), and naphthalene-2,3-dicarboxal-dehyde (NDA), all obtained from the Invitrogen (Carlsbad, CA, USA) to detect the O2−, ROS, and glutathione, respectively.
Different time frames and exposure times were examined to find the optimal conditions to see the differences between the test groups. After the treatment of L2 nematodes with 10× MIC concentration of each PBC and antibiotics in NGM plates at 20 °C, the O2− and ROS levels were measured after 4 days, while glutathione levels were measured after 24 h. The plates were washed, and the liquid was centrifuged (6000× g) 2 times with PBS. The nematodes were exposed to DHE (20 μM), DCF (10 μM), and NDA (20 μM) probes to measure the O2−, ROS, and glutathione, respectively. The nematodes were incubated with probes at 20 °C for 4 h. The liquid was gently washed 2 times with PBS buffer and the animals were examined on a fluorescence microscope (Zeiss axio imager Z1) with identical exposure time (1.5 s). Densitometry and intensity analysis was performed by Fiji software (ImageJ).
Pormohammad A., Hansen D, & Turner R.J. (2022). Antibacterial, Antibiofilm, and Antioxidant Activity of 15 Different Plant-Based Natural Compounds in Comparison with Ciprofloxacin and Gentamicin. Antibiotics, 11(8), 1099.
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